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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 120 (1994), S. 323-337 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Benthic bacterial production and biomass were measured at 16 stations in the North Sea covering a wide range of sediment types from the Southern Bight and the English coast to the Skagerrak. Stations were sampled in August 1991 and February 1992. The best predictor for summer/winter and spatial variations in benthic bacterial production in North Sea sediments was temperature. In winter the ranges in temperature were too small to account for the spatial variations in benthic bacterial production. The direct effect of temperature alone on bacterial production could not explain the variations. The apparent Q10-values derived from the relations between bacterial growth and temperature exceeded the range in Q10-values generally accepted for bacterial growth (between 2 and 3). Temperature was assumed to covary closely with substrate availability for bacteria. Due to its significant seasonality phytopigment content of the sediment (chlorophyll a and pheopigment) was found to be a better indicator of substrate availability than sediment organic matter, which did not demonstrate seasonality. Temperature and phytopigment accounted for up to 88% of the seasonal and spatial variations in bacterial production. The significant relations between bacterial production and biomass in summer coinciding with significant relations between bacterial biomass and phytopigments suggest that variations in phytopigments in the sediment may be indicators of the variability of labile components regulating bacterial production in sediments.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2013-04-26
    Description: Proximal promoter DNA methylation has been shown to be important for regulating gene expression. However, its relative contribution to the cell-specific expression of endothelial cell (EC)-enriched genes has not been defined. We used methyl-DNA immunoprecipitation and bisulfite conversion to analyze the DNA methylation profile of EC-enriched genes in ECs vs nonexpressing cell types, both in vitro and in vivo. We show that prototypic EC-enriched genes exhibit functional differential patterns of DNA methylation in proximal promoter regions of most (eg, CD31, von Willebrand factor [vWF], VE-cadherin, and intercellular adhesion molecule-2), but not all (eg, VEGFR-1 and VEGFR-2), EC-enriched genes. Comparable findings were evident in cultured ECs, human blood origin ECs, and murine aortic ECs. Promoter-reporter episomal transfection assays for endothelial nitric oxide synthase, VE-cadherin, and vWF indicated functional promoter activity in cell types where the native gene was not active. Inhibition of DNA methyltransferase activity indicated important functional relevance. Importantly, profiling DNA replication timing patterns indicated that EC-enriched gene promoters with differentially methylated regions replicate early in S-phase in both expressing and nonexpressing cell types. Collectively, these studies highlight the functional importance of promoter DNA methylation in controlling vascular EC gene expression.
    Keywords: Vascular Biology
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 3
    Publication Date: 2013-03-21
    Description: Short-term psychological impact of the BRCA1/2 test result in women with breast cancer according to their perceived probability of genetic predisposition to cancer British Journal of Cancer 108, 1012 (19 March 2013). doi:10.1038/bjc.2012.599 Authors: A Brédart, J L Kop, A DePauw, O Caron, S Sultan, D Leblond, A Fajac, B Buecher, M Gauthier-Villars, C Noguès, C Flahault, D Stoppa-Lyonnet & S Dolbeault
    Keywords: BRCA1/2 testinganxietydepressionstress reactionsperceived probability of genetic predisposition to cancer
    Print ISSN: 0007-0920
    Electronic ISSN: 1532-1827
    Topics: Medicine
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  • 4
    Publication Date: 2017-07-14
    Description: Introduction Retrievable inferior vena cava (IVC) filters have been increasingly used in patients with major trauma who have contraindications to anticoagulant prophylaxis as a primary prophylactic measure against venous thromboembolism (VTE). The benefits, risks and cost-effectiveness of such strategy are uncertain. Methods and analysis Patients with major trauma, defined by an estimated Injury Severity Score 〉15, who have contraindications to anticoagulant VTE prophylaxis within 72 hours of hospitalisation to the study centre will be eligible for this randomised multicentre controlled trial. After obtaining consent from patients, or the persons responsible for the patients, study patients are randomly allocated to either control or IVC filter, within 72 hours of trauma admission, in a 1:1 ratio by permuted blocks stratified by study centre. The primary outcomes are (1) the composite endpoint of (A) pulmonary embolism (PE) as demonstrated by CT pulmonary angiography, high probability ventilation/perfusion scan, transoesophageal echocardiography (by showing clots within pulmonary arterial trunk), pulmonary angiography or postmortem examination during the same hospitalisation or 90-day after trauma whichever is earlier and (B) hospital mortality; and (2) the total cost of treatment including the costs of an IVC filter, total number of CT and ultrasound scans required, length of intensive care unit and hospital stay, procedures and drugs required to treat PE or complications related to the IVC filters. The study started in June 2015 and the final enrolment target is 240 patients. No interim analysis is planned; incidence of fatal PE is used as safety stopping rule for the trial. Ethics and dissemination Ethics approval was obtained in all four participating centres in Australia. Results of the main trial and each of the secondary endpoints will be submitted for publication in a peer-reviewed journal. Trial registration number ACTRN12614000963628; Pre-results.
    Keywords: Open access, Intensive care
    Electronic ISSN: 2044-6055
    Topics: Medicine
    Published by BMJ Publishing
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  • 5
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 6
    Publication Date: 2015-03-06
    Description: Sulawesi comprises of about 182,870 km² of land and fewer botanical specimens (about 23 specimens per 100 km², Whitten et al., 1987) have been collected here than in any other major island in Indonesia. This island is up to date botanically poorly explored and according to Van Steenis (1950) about 32,500 specimens of plants were recorded, the number is probably only a rough estimation and certainly in the precomputer era not based on real specimens. Within the framework of STORMA (Stability of Rainforest Margins) we analyzed the vegetation in several plots of one hectare of different land use systems at the Lore Lindu National Park, Central Sulawesi, Indonesia. Rapidly we were faced with the problem that the identification of the mostly sterile trees would be almost impossible without a sound basic checklist. Because previous hardcopies (Hildebrand, 1950; Soewanda & Tantra, 1973; Whitmore et al., 1989) were either outdated or incomplete (especially for the small diameter trees) we decided to create a new one using data of actual plant specimens housed at the National Herbarium of the Netherlands, Universiteit Leiden branch (with duplicate specimens in several other herbaria like BO, E, K and others), and enter them in the BRAHMS (Botanical Research and Herbarium Management Systems) database developed by Denis Filer, University of Oxford. More than 120 woody families have been screened and the label information of all specimens (c. 13,000) checked and partly analysed. The density index calculated from our figures is very low (7) but for the first time underpinned by hard, retrievable data. As we did not include species — and specimens rich herbaceous families in our calculations (Orchidaceae, Poaceae, Zingiberaceae, ferns, etc.) the much lower collection index can be at least partially explained.
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
    Format: application/pdf
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  • 7
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 8
    Publication Date: 2024-01-12
    Description: Sulawesi comprises of about 182,870 km\xc2\xb2 of land and fewer botanical specimens (about 23 specimens per 100 km\xc2\xb2, Whitten et al., 1987) have been collected here than in any other major island in Indonesia. This island is up to date botanically poorly explored and according to Van Steenis (1950) about 32,500 specimens of plants were recorded, the number is probably only a rough estimation and certainly in the precomputer era not based on real specimens. Within the framework of STORMA (Stability of Rainforest Margins) we analyzed the vegetation in several plots of one hectare of different land use systems at the Lore Lindu National Park, Central Sulawesi, Indonesia. Rapidly we were faced with the problem that the identification of the mostly sterile trees would be almost impossible without a sound basic checklist. Because previous hardcopies (Hildebrand, 1950; Soewanda & Tantra, 1973; Whitmore et al., 1989) were either outdated or incomplete (especially for the small diameter trees) we decided to create a new one using data of actual plant specimens housed at the National Herbarium of the Netherlands, Universiteit Leiden branch (with duplicate specimens in several other herbaria like BO, E, K and others), and enter them in the BRAHMS (Botanical Research and Herbarium Management Systems) database developed by Denis Filer, University of Oxford.\nMore than 120 woody families have been screened and the label information of all specimens (c. 13,000) checked and partly analysed. The density index calculated from our figures is very low (7) but for the first time underpinned by hard, retrievable data. As we did not include species \xe2\x80\x94 and specimens rich herbaceous families in our calculations (Orchidaceae, Poaceae, Zingiberaceae, ferns, etc.) the much lower collection index can be at least partially explained.
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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