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Cloning and Functional Characterisation of Two Novel Nicotinic Acetylcholine Receptor α Subunits from the Insect Pest Myzus persicae (1998)

Sgard, F. ; Fraser, S. P. ; Katkowska, M. J. ; [et al.]

Oxford, UK : Blackwell Science Ltd

Journal of neurochemistry 71 (1998), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: Nicotinic acetylcholine receptors play a major role in excitatory neurotransmission in insect CNSs and constitute an important target for insecticides. Here, we report the isolation and functional characterisation of two cDNAs encoding nicotinic acetylcholine receptor α subunits from a major insect pest, the peach-potato aphid Myzus persicae. These two subunits, termed Mpα1 and Mpα2, are respective structural homologues of the Drosophila Dα2/Schistocerca gregariaαL1 α-subunit pair and the Drosophila ALS α subunit. Xenopus oocyte expression confirmed that each Myzus subunit can form functional acetylcholine- or nicotine-gated channels. However, some electrophysiological and pharmacological properties of the Myzus subunits were distinct from those encoded by the corresponding Drosophila subunits. Coexpression of the Myzus subunits with the chick β2 subunit revealed other differences from the Drosophila system, as only very limited potentiation of agonist-induced currents was observed with Mpα2 and none with Mpα1. Available data therefore indicate that structurally homologous insect nicotinic acetylcholine receptor α subunits from different species can exhibit distinctive physiological and pharmacological characteristics.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1471-4159.1998.71030903.x

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The effect of external cesium ions on the muscle fibre resting potential in mealworm larva (Tenebrio molitor L.) (1995)

Katkowska, M. J.

Springer

Journal of comparative physiology 177 (1995), S. 519-526

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ISSN: 1432-1351

Keywords: Cesium ; Membrane potential ; Blocking of potassium inward current ; Insect muscle

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The membrane potential of ventral longitudinal muscles of Tenebrio molitor larvae was studied as a function of time and of cesium substituted for all or part of external potassium. The conventional microelectrode technique was applied. The mean value of resting potential was — 47.4 mV in standard physiological saline which did not change significantly with time (90 min). Cesium caused, almost immediately, a significant hyperpolarization of membrane potential the magnitude of which depended on cesium concentration. The presence of external potassium enhanced the effectiveness of cesium action, resulting in more pronounced hyperpolarization. The effect of Cs ions was fully reversible upon washing. These data support the idea that inward potassium current can be activated at resting potential level, at least in some cells, including the muscles studied. It is presumed that this potassium current might have some contribution to the resting membrane potential generation in mealworm larva muscles.