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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 33 (1988), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 33 (1988), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A netting and hydrographic study of Preston dock was undertaken between May 1987 and April 1988. Fish populations were assessed using Lundgren monofilament multimesh gillnets. The dock was a low-salinity environment regularly replenished with estuarine water on spring tides. Influxes of higher-salinity water caused intermittent saline wedges in the deeper regions. The dock water was poorly mixed in the summer, resulting in bottom water anoxia. The water was eutrophic, and blue-green algal blooms were common for much of the year.Fourteen freshwater, marine-estuarine, anadromous and catadromous species were present in the dock. No seasonal patterns in diversity were apparent, due to the dominance of the herring population. Changes in species composition and abundance seem to relate to seasonal migratory patterns and, in the case of the freshwater fish, estuarine salinity, rather than to water quality.The suitablity of docks for sampling estuarine fish assemblages is discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 74 (1988), S. 403-408 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In cultures of tsetse proboscis stages during the development of Trypanosoma congolense, attached epimastigote forms multiply and give rise to free nondividing metacyclic trypanosomes. Prevention of attachment by shaking the cultures or by providing a polypropylene substratum does not inhibit epimastigote division but does prevent the differentiation of metacyclics. We conclude that epimastigote attachment forms a necessary part of the program of metacyclic development.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 281 (1995), S. 93-99 
    ISSN: 1432-0878
    Keywords: Key words: Protein synthesis ; Keratin ; Actin ; Hoof ; Tissue culture ; Lameness ; Cow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The mechanical strength of the bovine hoof depends on keratinization of cells in the germinal layers of the epidermis. Histological examination of hoof tissues in calves and young heifers has identified disturbances in this keratinization process which would result in ineffective hoof development and could precipitate lameness. Short-term culture of bovine hoof tissue was used to investigate epidermal keratinization. Cell function remains viable in these cultures. The rate of protein synthesis, measured by [35S]-methionine incorporation, continued for at least 3 h in culture. Radiolabelled proteins in tissue homogenates were separated by SDS-polyacrylamide gel electrophoresis and characterised by fluorography and were representative of the proteins found in hoof tissue. Three prominent radiolabelled bands were identified as keratins and actin by Western blotting. Immunohistochemistry showed that keratin was localised principally in the epidermal layers, and microautoradiography indicated that this was the major site of protein synthesis. Hoof tissues cultured under these conditions provide a useful system for studying the acute regulation of epidermal keratinization.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 281 (1995), S. 93-99 
    ISSN: 1432-0878
    Keywords: Protein synthesis ; Keratin ; Actin ; Hoof ; Tissue culture ; Lameness ; Cow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mechanical strength of the bovine hoof depends on keratinization of cells in the germinal layers of the epidermis. Histological examination of hoof tissues in calves and young heifers has identified disturbances in this keratinization process which would result in ineffective hoof development and could precipitate lameness. Short-term culture of bovine hoof tissue was used to investigate epidermal keratinization. Cell function remains viable in these cultures. The rate of protein synthesis, measured by [35S]-methionine incorporation, continued for at least 3 h in culture. Radiolabelled proteins in tissue homogenates were separated by SDS-polyacrylamide gel electrophoresis and characterised by fluorography and were representative of the proteins found in hoof tissue. Three prominent radiolabelled bands were identified as keratins and actin by Western blotting. Immunohistochemistry showed that keratin was localised principally in the epidermal layers, and microautoradiography indicated that this was the major site of protein synthesis. Hoof tissues cultured under these conditions provide a useful system for studying the acute regulation of epidermal keratinization.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2024-02-07
    Description: Biological productivity in the ocean directly influences the partitioning of carbon between the atmosphere and ocean interior. Through this carbon cycle feedback, changing ocean productivity has long been hypothesized as a key pathway for modulating past atmospheric carbon dioxide levels and hence global climate. Because phytoplankton preferentially assimilate the light isotopes of carbon and the major nutrients nitrate and silicic acid, stable isotopes of carbon (C), nitrogen (N), and silicon (Si) in seawater and marine sediments can inform on ocean carbon and nutrient cycling, and by extension the relationship with biological productivity and global climate. Here, we compile water column C, N, and Si stable isotopes from GEOTRACES-era data in four key ocean regions to review geochemical proxies of oceanic carbon and nutrient cycling based on the C, N, and Si isotopic composition of marine sediments. External sources and sinks as well as internal cycling (including assimilation, particulate matter export, and regeneration) are discussed as likely drivers of observed C, N, and Si isotope distributions in the ocean. The potential for C, N, and Si isotope measurements in sedimentary archives to record aspects of past ocean C and nutrient cycling is evaluated, along with key uncertainties and limitations associated with each proxy. Constraints on ocean C and nutrient cycling during late Quaternary glacial-interglacial cycles and over the Cenozoic are examined. This review highlights opportunities for future research using multielement stable isotope proxy applications and emphasizes the importance of such applications to reconstructing past changes in the oceans and climate system.
    Type: Article , PeerReviewed
    Format: text
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  • 7
    Publication Date: 2016-06-09
    Description: ABSTRACT Characterizing the hydraulic conductivity (K) of aquitards is difficult due to technical and logistical difficulties associated with field-based methods as well as the cost and challenge of collecting representative and competent core samples for laboratory analysis. The objective of this study was to produce a multi-scale comparison of vertical and horizontal hydraulic conductivity (K v and K h , respectively) of a regionally extensive Cretaceous clay-rich aquitard in southern Saskatchewan. Ten vibrating wire pressure transducers were lowered into place at depths between 25 and 325 m, then the annular was space was filled with a cement-bentonite grout. The in situ K h was estimated at the location of each transducer by simulating the early-time pore pressure measurements following setting of the grout using a 2D axisymmetric, finite element, numerical model. Core samples were collected during drilling for conventional laboratory testing for K v to compare with the transducer-determined in situ K h . Results highlight the importance of scale and consideration of the presence of possible secondary features (e.g. fractures) in the aquitard. The proximity of the transducers to an active potash mine (∼1 km) where depressurization of an underlying aquifer resulted in drawdown through the aquitard provided a unique opportunity to model the current hydraulic head profile using both the K h and K v estimates. Results indicate that the transducer-determined K h estimates would allow for the development of the current hydraulic head distribution, and that simulating the pore pressure recovery can be used to estimate moderately low in situ K h (〈10 −11 ms −1 ). This article is protected by copyright. All rights reserved.
    Print ISSN: 0043-1397
    Electronic ISSN: 1944-7973
    Topics: Architecture, Civil Engineering, Surveying , Geography
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  • 8
    Publication Date: 2019-07-16
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 9
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    ELSEVIER SCIENCE BV
    In:  EPIC3Earth and Planetary Science Letters, ELSEVIER SCIENCE BV, 438, pp. 122-129, ISSN: 0012-821X
    Publication Date: 2016-02-15
    Description: Major shifts in ocean circulation are thought to be responsible for abrupt changes in temperature and atmospheric CO2 during the last deglaciation, linked to variability in meridional heat transport and deep ocean carbon storage. There is also widespread evidence for shifts in biological production during these times of deglacial CO2 rise, including enhanced diatom production in regions such as the tropical Atlantic. However, it remains unclear as to whether this diatom production was driven by enhanced wind-driven upwelling or density-driven vertical mixing, or by elevated thermocline concentrations of silicic acid supplied to the surface at a constant rate. Here, we demonstrate that silicic acid supply at depth in the NE Atlantic was enhanced during the abrupt climate events of the deglaciation. We use marine sediment archives to show that an increase in diatom production during abrupt climate shifts could only occur in regions of the NE Atlantic where the deep supply of silicic acid could reach the surface. The associated changes are indicative of enhanced regional wind-driven upwelling and/or weakened stratification due to circulation changes during phases of weakened Atlantic meridional overturning. Globally near-synchronous pulses of diatom production and enhanced thermocline concentrations of silicic acid suggest that widespread deglacial surface-driven breakdown of stratification, linked to changes in atmospheric circulation, had major consequences for biological productivity and carbon cycling.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 10
    Publication Date: 2019-04-26
    Description: The west Antarctic Peninsula (WAP) region has undergone significant changes in temperature and seasonal ice dynamics since the mid-twentieth century, with strong impacts on the regional ecosystem, ocean chemistry and hydrographic properties. Changes to these long-term trends of warming and sea ice decline have been observed in the 21st century, but their consequences for ocean physics, chemistry and the ecology of the high-productivity shelf ecosystem are yet to be fully established. The WAP shelf is important for regional krill stocks and higher trophic levels, whilst the degree of variability and change in the physical environment and documented biological and biogeochemical responses make this a model system for how climate and sea ice changes might restructure high-latitude ecosystems. Although this region is arguably the best-measured and best-understood shelf region around Antarctica, significant gaps remain in spatial and temporal data capable of resolving the atmosphere-ice-ocean-ecosystem feedbacks that control the dynamics and evolution of this complex polar system. Here we summarise the current state of knowledge regarding the key mechanisms and interactions regulating the physical, biogeochemical and biological processes at work, the ways in which the shelf environment is changing, and the ecosystem response to the changes underway. We outline the overarching cross-disciplinary priorities for future research, as well as the most important discipline-specific objectives. Underpinning these priorities and objectives is the need to better-define the causes, magnitude and timescales of variability and change at all levels of the system. A combination of traditional and innovative approaches will be critical to addressing these priorities and developing a co-ordinated observing system for the WAP shelf, which is required to detect and elucidate change into the future.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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