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  • 1
    Electronic Resource
    Electronic Resource
    An Agrobacterium virulence factor encoded by a Ti plasmid gene or a chromosomal gene is required for T-DNA transfer into plants (1995)
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Scientific Publications
    Molecular microbiology 17 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Mutagenesis of the vir region on the Ti plasmid of Agrobacterium tumefaciens revealed a new locus, virJ, that is induced by the plant-wound signal molecule, acetosyringone (AS). virJ lies between virA and virB, and is transcribed in the same direction. The amino acid sequence of virJ is similar to a region of a previously characterized chromosomal gene, acvB, required for virulence. virJ can complement the avirulent phenotype of an acvB mutant, indicating that virJ and acvB encode the same factor required for tumorigenesis. Southern analysis revealed that virJ is present on the Ti plasmid of an octopine but not a nopaline strain whereas acvB is present on the chromosomes of both octopine and nopaline strains. While virJ is regulated by AS under the control of the virA/virG two-component regulatory system, acvB is not induced by AS. VirJ possesses a putative signal peptide and was found predominantly in the periplasmic fraction. The strain lacking both acvB and virJ had an impaired ability to transfer T-DNA into plant cells, suggesting that the factor encoded by virJ or acvB is required for T-DNA transfer from A. tumefaciens to plant cells. acvB is the first chromosomal gene implicated in T-DNA transfer, but whether it functions specifically for this process is not clear. We hypothesize that virJ evolved from acvB, presumably for a more specialized role in tumorigenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_17020259.x
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  • 2
    Electronic Resource
    Electronic Resource
    Root organogenesis from single cells released from the root cap of Medicago sp. (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 303-308 
    Details
    ISSN: 1573-5044
    Keywords: Alfalfa ; alfalfa tissue culture ; root border cells ; root cap ; sloughed root cap cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00157595
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of transferred genes during hairy root development in pea (1995)
    Springer
    Plant cell, tissue and organ culture 42 (1995), S. 57-66 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium rhizogenes ; border cells ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root border cell development and expression of reporter genes were evaluated in transgenic pea hairy roots. Successful induction of hairy roots in pea is conditioned by bacterial strain and plant genotype, as well as by developmental and environmental factors. Morphological changes sometimes occur when hairy roots are transferred from infected plants to tissue culture media, but such changes are confined to specific clones. Expression of reporter genes under the control of promoters from bean (Phaseolus vulgaris L.) “stress” genes encoding phenylalanine ammonia lyase and chalcone synthase were evaluated. Expression patterns vary between hairy roots taken directly from infected plants, and those grown in culture; most hairy roots taken from infected plants exhibit expression throughout all tissues, whereas expression in cultured hairy roots is most often localized to specific tissues. Patterns of expression that occur during different stages of hairy root development are very similar to those observed in transgenic plants expressing the same fusion genes. Border cell separation and release in hairy roots is normal, and expression of glucuronidase in border cells of some transgenic roots resulted in development of bright blue single cells. Cultured hairy roots should provide a very useful model for studying the effect of defined changes in root border cells on microbial associations with roots of this important legume.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037682
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular cloning and expression of mRNAs encoding H1 histone and an H1 histone-like sequences in root tips of pea (Pisum sativum L.) (1995)
    Springer
    Plant molecular biology 28 (1995), S. 1143-1147 
    Details
    ISSN: 1573-5028
    Keywords: globular domain ; H1 histone ; pea ; repeating motif of amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones representing mRNAs, highly expressed in pea root tips, were isolated by mRNA differential display. Ribonuclease protection analyses showed different patterns of expression of these two messages in several pea tissues. Sequence analysis showed that the first clone, PsH1b-40, has 100% homology with a previously isolated H1 histone cDNA, PsH1b. However, it has an additional 30 nt at the 3′ end which is absent in PsH1b, suggesting possible multiple polyadenylation sites in the same mRNA. The second clone, PsH1b-41, encodes a deduced 19.5 kDa protein of 185 amino acids with an isoelectric point of 11.5. The putative globular domain of the encoded protein showed 67–71% residue identity with globular domains of 28 kDa pea PsH1b H1 histone and Arabidopsis thaliana H1-1 H1 histone. It has 9 repeating motifs of (T/S)XXK. In the C-terminal domain, there are four lysine-rich repeating motifs of SXK(T/S)PXKKXK which may be involved in chromatin condensation and decondensation. Southern blot analysis of nuclear DNA shows that PsH1-41 belongs to a multigene family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032675
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning of genes whose expression is correlated with mitosis and localized in dividing cells in root caps of Pisum sativum L. (1997)
    Springer
    Plant molecular biology 35 (1997), S. 1045-1051 
    Details
    ISSN: 1573-5028
    Keywords: root cap cell differentiation ; hydroxyproline-rich glycoprotein ; callus protein P23 ; ribosomal protein L41 ; whole mount in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Removal of border cells from pea roots synchronizes and induces root cap cell division, wall biogenesis and differentiation. Three messages which are expressed differentially in such induced root caps have been cloned. Sequence analyses showed that the PsHRGP1-encoded protein has high homology with a hydroxyproline-rich glycoprotein. The PsCaP23-encoded protein has high homology with an alfalfa callus protein or translationally controlled human or mouse tumor protein P23. The PsRbL41-encoded protein has high homology with a highly basic 60S ribosomal protein L41. In situ hybridization showed that PsHRGP1, PsCaP23 and PsRbL41 messages are localized within dividing cells of the root cap. PsHRGP1 is highly expressed in uninduced root caps, but its message is repressed by 10–11 times as soon as cell division and differentiation begin. Expression of PsHRGP1 recovers to higher than (180%) its initial level in 30 min. PsHRGP1 is root-specific. PsCaP23 and PsRbL41 messages increase ca. 3-fold within 15 min after root cap induction. All three genes represent small families of 3–5 closely related genes in the pea genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005930625920
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  • 6
    Electronic Resource
    Electronic Resource
    Reduced rhizosphere colonization ability ofAgrobacterium tumefaciens chromosomal virulence (chv) mutants (1989)
    Springer
    Plant and soil 113 (1989), S. 129-132 
    Details
    ISSN: 1573-5036
    Keywords: Agrobacterium tumefaciens ; binding ; Pisum sativum ; rhizosphere ; root cap cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mutations in the chromosomal virulence (chv) region ofA. tumefaciens strain A723 reduce virulence, motility, and ability of the bacteria to bind to plant cells. We conducted experiments to assess the ability ofchv mutants to colonize the rhizosphere ofPisum sativum. The mutation had no effect on ability of bacteria to grow with a defined number of root cap cells as the sole carbon and nitrogen source. Ten days after inoculation, there were up to 103-fold more wild type thanchv mutant bacteria present in the rhizosphere of inoculated plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181931
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