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  • 1
    Electronic Resource
    Electronic Resource
    The effect of immersion on haemodynamic and fetal measures in uncomplicated pregnancies of nulliparous women (2000)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 107 (2000), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To evaluate the effect of immersion in water on maternal haemodynamic measures and fetal heart rate patterns in healthy nulliparous women.Design A randomised cross-over study.Participants Twenty pregnant women were studied between 26–29 weeks and between 34–37 weeks of gestation.Methods The women were either immersed in water for 35 minutes or rested on a bed for a similar period of time. Maternal blood pressure, thoracic electrical bioimpedance, urine and blood samples, and fetal heart rate patterns were obtained before and after immersion or bedrest.Results Compared with bedrest and pre-immersion values, immersion resulted in a significant decrease in blood pressure, which returned to baseline values within 1.5 hour. Urine production increased for a short period after immersion, but 24 hour samples showed no difference compared with bedrest. Sodium, potassium and creatinine levels in urine remained constant. No significant changes were found for the other haemodynamic measures. There were no effects on fetal heart rate nor on its variation.Conclusion In healthy pregnant women immersion for 35 minutes has a clear effect on blood pressure and urine production. However, these effects are short lasting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.2000.tb13310.x
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  • 2
    Electronic Resource
    Electronic Resource
    Electrophoretic analyses and denaturation tests of the haemoglobins of Trachurus trachurs (L.) (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 9 (1976), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A total of 210 samples of haemoglobin of Trachurus trachurus (L.) caught in the Gulf of Trieste in 1972–1973, were analysed in search of haemoglobin polymorphism. Genetic polymorphism may be used as a genetic marker to identify sub–populations on the basis of allele frequencies.All the samples analysed produced the same haemoglobinic pattern, composed of six bands. The quantitative differences noted between the various fractions were not sufficient to demonstrate the existence of different phenotypes.Denaturation tests carried out by treating erythrocytes with non–sterile physiological solutions or by keeping the haemolysate at room temperature before electrophoresis, resulted in new patterns. This result shows the importance of the careful treatment of blood samples to assure reliable results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1976.tb04688.x
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  • 3
    Electronic Resource
    Electronic Resource
    Specific inhibition of mitochondrial Ca 2+ transport by antibodies directed to the Ca 2+ -binding glycoprotein (1976)
    [s.l.] : Nature Publishing Group
    Nature 264 (1976), S. 185-186 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Rabbits were immunised using 2 mg of purified ox liver glycoprotein isolated in our laboratory as before7. Antigen plus Freund's complete adjuvant was given in five injections into all four legs and the neck. The operation was repeated with Freund's incomplete adjuvant a month later. A secondary ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/264185a0
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  • 4
    Electronic Resource
    Electronic Resource
    Para and Meta-Substituted Benzeneseleninic Acids Interactions with Cr(III), Mn(II), Fe(II), Fe(III), Sn(II) and Ag(I)
    Amsterdam : Elsevier
    Inorganica Chimica Acta 40 (1980), S. X120 
    Details
    ISSN: 0020-1693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0020-1693(00)92220-5
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  • 5
    Electronic Resource
    Electronic Resource
    DNA homology comparison between American and European Borrelia burgdorferi strains (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 76 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seven strains of Borrelia burgdorferi isolated from ticks and from human beings in Europe and U.S.A. were analyzed for DNA restriction patterns with several enzymes and for DNA homology in Southern blot hybridizations. The restriction patterns showed a moderately high variability. In Southern blot hybridization, strain B31 (U.S.A.) DNA gave a strong signal with itself, strains Bsf (U.S.A.) and Alcaide (isolated in Italy but presumably contracted in Venezuela). Strain B45 (F.R.G.) hybridized to itself, strain BITS (Italy) and to strain D.A. (Italy). Strain Nancy (Italy) gave a signal only when hybridized to itself, although it was classified as Borrelia on the basis of the clinical manifestations, SDS-PAGE protein pattern and antigenic determinants. No hybridization differences were observed for strains isolated from different hosts in the same continental geographical area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04157.x
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  • 6
    Electronic Resource
    Electronic Resource
    Restriction endonuclease analysis of four Borrelia burgdorferi strains (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 47 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi: one isolated from man (SF), one from Ixodes dammini (B31) and two form I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb02443.x
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of Y-chromosomal STRs: a multicenter study (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 125-133 
    Details
    ISSN: 1437-1596
    Keywords: Key words Short tandem repeats ; Y chromosome ; Population analysis ; Mutation rate ; Y haplotype ; analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract A multicenter study has been carried out to characterize 13 polymorphic short tandem repeat (STR) systems located on the male specific part of the human Y chromosome (DYS19, DYS288, DYS385, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, YCAI, YCAII, YCAIII, DXYS156Y). Amplification parameters and electrophoresis protocols including multiplex approaches were compiled. The typing of non-recombining Y loci with uniparental inheritance requires special attention to population substructuring due to prevalent male lineages. To assess the extent of these subheterogeneities up to 3825 unrelated males were typed in up to 48 population samples for the respective loci. A consistent repeat based nomenclature for most of the loci has been introduced. Moreover we have estimated the average mutation rate for DYS19 in 626 confirmed father-son pairs as 3.2 × 10–3 (95% confidence interval limits of 0.00041–0.00677), a value which can also be expected for other Y-STR loci with similar repeat structure. Recommendations are given for the forensic application of a basic set of 7 STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393) for standard Y-haplotyping in forensic and paternity casework. We recommend further the inclusion of the highly polymorphic bilocal Y-STRs DYS385, YCAII, YCAIII for a nearly complete individualisation of almost any given unrelated male individual. Together, these results suggest that Y-STR loci are useful markers to identify males and male lineages in forensic practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050051
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  • 8
    Electronic Resource
    Electronic Resource
    Chromosome Y microsatellites: population genetic and evolutionary aspects (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 134-140 
    Details
    ISSN: 1437-1596
    Keywords: Key words Y chromosome ; Haplotypes ; Evolution ; Population studies ; Genetic affinities ; STR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract By means of a multicenter study, a large number of males have been characterized for Y-chromosome specific short tandem repeats (STRs) or microsatellites. A complete summary of the allele frequency distributions for these Y-STRs is presented in the Appendix. This manuscript describes in more detail some of the population genetic and evolutionary aspects for a restricted set of seven chromosome Y STRs in a selected number of population samples. For all the chromosome Y STRs markedly different region-specific allele frequency distributions were observed, also when closely related populations were compared. Haplotype analyses using AMOVA showed that when four different European male groups (Germans, Dutch, Swiss, Italians) were compared, less than 10% of the total genetic variability was due to differences between these populations. Nevertheless, these pairwise comparisons revealed significant differences between most population pairs. Assuming a step-wise mutation model and a mutation frequency of 0.21%, it was estimated that chromosome Y STR-based evolutionary lines of descent can be reliably inferred over a time-span of only 1950 generations (or about 49000 years). This reduces the reliability of the inference of population affinities to a historical, rather than evolutionary time scale. This is best illustrated by the construction of a human evolutionary tree based on chromosome Y STRs in which most of the branches connect in a markedly different way compared with trees based on classical protein polymorphisms and/or mtDNA sequence variation. Thus, the chromosome Y STRs seem to be very useful in comparing closely related populations which cannot probably be separated by e.g. autosomal STRs. However, in order to be used in an evolutionary context they need to be combined with more stable Y-polymorphisms e.g. base-substitutions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050052
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  • 9
    Electronic Resource
    Electronic Resource
    Differential responses to ultraviolet irradiation of the polar cytoplasm ofDrosophila eggs (1974)
    Springer
    Development genes and evolution 175 (1974), S. 1-11 
    Details
    ISSN: 1432-041X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The posterior pole ofDrosophila melanogaster eggs was irradiated with ultraviolet light of 253.7 nm wavelength; different batches were irradiated at different times after oviposition, ranging from less than 10 mins to 125 mins. Two different experiments were run at different dose levels: 280 μW/mm2 and 530 μW/mm2. A differential response to irrariation was observed in relation to the age of the treated eggs. Embryo mortality increased with egg age in both experiments (Fig. 1). No differential effects onlarval mortality were found in egg batches irradiated between 15 and 95 mins of age in either experiment (Fig. 3). The incidence ofsterility in the survivors was higher when the eggs were irradiated before 55 mins or when pole nuclei were present. The overall incidence of sterility was much higher in the high-dose experiment (Fig. 4). Sterility is considered as a measure of damage to germ cell precursors, most probably involving RNA. On this basis the probability of the affected RNA being messenger RNA stored in polar granules is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00573220
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  • 10
    Electronic Resource
    Electronic Resource
    Differential responses to ultraviolet irradiation of the polar cytoplasm ofDrosophila eggs: II. Response to dose (1976)
    Springer
    Development genes and evolution 179 (1976), S. 291-300 
    Details
    ISSN: 1432-041X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The posterior pole cytoplasm of early embryos ofDrosophila melanogaster was irradiated with increasing doses of U.V. light at a wave length specific for nucleic acids 35 and 75 min after ovoposition, when nuclei were still inside the yolk mass. Probit analysis showed that: Sterility was higher in irradiations performed at 35 min. Mortality was higher in the experiment performed at 75 min. After treatment at 35 min, sterility and mortality were independent phenomena. The numbers of targets involved in germ cell determination were of the order of tens and hundreds at 35 and 75 min respectively. The numbers of targets involved in viability were of several orders of magnitude in both developmental stages. The irradiation, even at very low doses, caused a background effect which was probably due to “old” embryos. The sex ratio was distorted in favour of females at high doses and of males at low doses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848238
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