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  • 1
    Electronic Resource
    Electronic Resource
    Use of RAPD and microsatellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa, Medicago sativa (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The level of random amplified polymorphic DNA (RAPD) and microsatellite variation present in four ecotypes and two varieties of alfalfa (lucerne) from Italian and Egyptian germplasm sources was evaluated. A sample of 100 plants from 10 populations was analysed by means of 41 RAPD markers and 37 simple sequence repeat (SSR) markers. Both molecular approaches revealed a high degree of genetic diversity within each of the cultivated populations and enabled each of the plants considered to be uniquely fingerprinted. The genetic relationships among plants and populations were analysed by computing AMOVA (analysis of molecular variance) and FST analyses. RAPDs were able to separate the Italian populations from the Egyptian variety. SSRs allowed strong separation of the four Italian alfalfa ecotypes. It was concluded that RAPD and microsatellites could be useful and powerful tools for assessing genetic variation and genetic relationships in tetraploid alfalfa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00501.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of polymorphism and influence of sample preparation on the infra-red spectrum of estradiol-17β
    Amsterdam : Elsevier
    Spectrochimica Acta 9 (1957), S. 346-356 
    Details
    ISSN: 0371-1951
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0371-1951(57)80150-7
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of nesosteine on Herxheimer microshock in guinea-pigs (1987)
    Springer
    Inflammation research 20 (1987), S. 327-329 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In this paper we firstly report the inhibitory effect of nesosteine, a mucolytic drug, on Herxheimer microshock in guinea-pigs. Nesosteine (5–50 mg/kg) is able to protect sensitized animals from ovalbumin-induced bronchospasm. On the other hand, the drug is ineffective against the bronchospasm induced by histamine and acetylcholine. These results have also been confirmed inin vitro experiments where it has been demonstrated that nesosteine (10−5 M) inhibits ovalbumin-induced histamine release in the trachea of sensitized animals. In the same preparation, the drug is ineffective against the contractions induced by histamine or acetylcholine. In conclusion, the drug presented here may be helpful in pathological conditions where reductions both of mucolysis and bronchospasm are sought.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02074704
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  • 4
    Electronic Resource
    Electronic Resource
    Videolaparoscopic cholecystectomy induces a hemostasis activation of lower grade than does open surgery (2000)
    Springer
    Surgical endoscopy and other interventional techniques 14 (2000), S. 170-174 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Blood clotting activation — Open and videolaparoscopic cholecystectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Tissue injury after trauma and surgery may induce alterations in blood coagulation and fibrinolysis, and the hypercoagulable state observed after surgery can be associated with the risk of postoperative thromboembolic complications. Recently, videolaparoscopic (VLPS) cholecystectomy has been introduced, and its advantages over the open procedure seem related to the reduced surgical trauma. The aim of this study was to investigate hemostatic system alterations in patients who undergo open and VLPS cholecystectomy. Methods: Fibrinogen, prothrombin fragment F1+2, D-dimer, and plasminogen activator inhibitor type-1 (PAI-1) activity was determined in 10 patients who underwent open (group A) and 10 patients who underwent VLPS cholecystectomy (group B), respectively. Blood samples were obtained the day of surgery in the morning (B1), after anesthesia (A1), 1 hour after the start of surgery (S1), then 30 min (E.05) and 24 h (E.24) after the surgery. Results: No significant differences were observed in baseline values between groups A and B for the parameters investigated. At 24 h after surgery, fibrinogen increased significantly (p 〈 0.05) in group A and also was significantly higher than in group B (p 〈 0.05). In group A, a marked increase in F1+2 levels (p 〈 0.01) was observed in all the samples, with the maximum values on the first day after surgery (3.7 nmol/l; 1.2–6.0 nmol/l), whereas in group B, a slight but significant increase in F1+2 levels (2.1 nmol/l; 1.1–3.9 nmol/l; p 〈 0.01) was observed only 30 min after the end of surgery. In both groups A and B, D-dimer markedly increased after surgery, without statistical significant differences between the two groups at any time. The PAI-1 activity plasma levels remained in the normal range during and after surgery in both groups. Conclusions: These results indicate that VLPS surgery induces an activation of the clotting system that, although of low degree and short duration, can lead to a transient prothrombotic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649900093
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  • 5
    Electronic Resource
    Electronic Resource
    Interleukin-6 and acute-phase proteins in head and neck cancer (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 159-162 
    Details
    ISSN: 1434-4726
    Keywords: Interleukin-6 ; Head and neck cancer ; Acute-phase response ; Fibrinogen ; C-reactive protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The acute-phase response is the answer of the organism to a disturbance of its homeostasis and is characterized by dramatic changes in the concentration of some plasma proteins defined as acute-phase proteins. In recent years several data have shown that interleukin-6 (IL-6) is the major inducer of acute-phase protein synthesis in human hepatocytes. Recently, we demonstrated higher IL-6 serum levels in head and neck cancer (HNC) patients than in healthy subjects. In the present study we examined the relationship between levels of IL-6 and of several acute-phase proteins, including C-reactive protein (CRP), α1-antitrypsin (AAT), αl-acid glycoprotein (AAG), haptoglobin (HPT) and fibrinogen. Eighteen patients were studied and had squamous cell carcinoma of the larynx (n = 9), oral cavity (n = 4), oropharynx (n = 3) and hypopharynx (n = 2). Proteins were measured at three time points before and three time points after surgery. Significant (P 〈 0.0001) relationships were found between IL-6 and CRP (r = 0.69), and fibrinogen (r = 0.51), whereas no correlation was found with AAT (r = 0.13, P = 0.56), AAG (r = 0.38; P = 0.07) and HPT (r = 0.16; P = 0.46). These data strongly suggest that IL-6 may play a key role in acute-phase protein synthesis in HNC and in regulation of the complex host response to malignancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178104
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro complement activation after contact with pyrolytic carbon-coated and uncoated polyethylene terephthalate (1997)
    Springer
    Journal of materials science 8 (1997), S. 771-774 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract This study was undertaken to evaluate whether the pyrolytic carbon coating of polyethylene terephthalate induces complement activation. Complement activation induced by pyrolytic carbon-coated polyethylene terephthalate (PET+PC) in comparison with uncoated polyethylene terephthalate (PET) was assessed on whole blood collected with heparin. The activation of the classic pathway was evaluated by C4d fragment enzyme immunoassay. The activation of the alternative pathway was evaluated with Bb fragment enzyme immunoassay. The results show that uncoated PET activates the alternative pathway, but not the classic one. PET+PC does not induce complement activation, not even through the alternative pathway. Pyrolytic carbon coating therefore contributes to improving blood compatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018564629533
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  • 7
    Electronic Resource
    Electronic Resource
    Cell death induced by metal ions: necrosis or apoptosis? (1998)
    Springer
    Journal of materials science 9 (1998), S. 31-37 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract We have evaluated if the cytotoxic effects of metals released from implants are due to necrosis or apoptosis. Peripheral blood mononuclear cells were exposed to different concentrations of chromium, nickel and cobalt extracts and the characteristics of both apoptosis and necrosis were evaluated by flow-cytometry at different culture endpoints. In order to define the prevalence of apoptosis or necrosis, the ratio cell death/apoptosis was calculated. A ratio of ≤1 means the prevalence of apoptotic events; a ratio 〉1 indicates the acute toxicity of the tested substance (necrosis). The extracts of chromium, cobalt and nickel had a cytotoxic effect on the mononuclear cells; high concentrations of cobalt and nickel produced cell necrosis, whereas by lowering the extract concentration apoptotic phenomena were observed. High chromium concentrations can induce cell death by apoptosis. Our data suggest that when large amounts of nickel and cobalt are released from implanted metal devices, necrosis is produced and consequently a strong inflammatory tissue reaction is likely to occur. The release of either chromium or limited amounts of nickel and cobalt induces toxicity characterized by apoptotic phenomena, which allows an adaptation of the tissue to the implant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008878527233
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  • 8
    Electronic Resource
    Electronic Resource
    Clinical diagnosis of mycobacterial diseases versus autopsy findings in 350 patients with AIDS (1996)
    Springer
    European journal of clinical microbiology & infectious diseases 15 (1996), S. 453-458 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three-hundred fifty consecutively autopsied AIDS patients in Milan, Italy, were studied to determine the frequencies of clinical and postmortem diagnoses of mycobacterial diseases, to evaluate the clinical histories of those patients with mycobacterial diseases, and to investigate the reasons for nondiagnosis of mycobacterial diseases during life. Seventy-eight patients (22.3%) had mycobacterial diseases. In 64 cases (18.3%) the diagnosis was made antemortem and in 50 (14.2%) at autopsy; there were 36 cases of concordant clinical and postmortem diagnoses. Nontuberculous mycobacterioses (NTM) were diagnosed in 41 patients (20 clinical/postmortem diagnoses, 11 clinical diagnoses, and 10 postmortem diagnoses), extrapulmonary tuberculosis (TB) in 19 patients (7 clinical/postmortem, 8 clinical, 4 postmortem), and pulmonary TB in 18 patients (9 clinical/postmortem, 9 clinical). Patients with a clinical diagnosis of mycobacteriosis but with no pathological evidence of disease at autopsy were considered to have recovered on the basis of negative culture findings and prolonged antimycobacterial treatment. In Italian patients with AIDS, NTM occurs less frequently and TB more frequently than in American AIDS patients. At least some of the patients reported in this study seemed to have recovered from mycobacterial disease after prolonged treatment. The lack of diagnosis during life can be attributed to aspecific symptoms, a rapidly terminal course, and the presence of concomitant opportunistic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01691311
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  • 9
    Electronic Resource
    Electronic Resource
    False positive results in cytotoxicity testing due to unexpectedly volatile compounds (1998)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 39 (1998), S. 286-291 
    Details
    ISSN: 0021-9304
    Keywords: cytotoxicity ; biomaterials ; standards ; microplate ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: We investigated the cytotoxicity of different dental materials according to the study protocol adopted by our lab for the screening of new materials. Experimental parameters used in such testing are addressed mainly in documents EN 30993 “Biological evaluation of medical devices, Part 5: Tests for cytotoxicity: in vitro methods” and “Biological evaluation of medical devices, Part 12: Sample preparation and reference materials.” Cells were cultured in microplates and challenged with aqueous extracts of the materials. The assay methods were neutral red- and propidium iodide-uptake assays, both indicative of cell viability and able to provide quantitative data. The observation of contrasting results for one material using the above-mentioned methods raised some concern about the assay system used. With further experimentation, it appeared that a sustained release of volatile substances still present in one extract exerted a toxic effect in neighboring cultures. It is concluded that in the microenvironment of a microplate the distribution of samples cannot be disregarded, as it may be responsible for toxicity cross-contamination. Moreover, the use of more than one single method has to be recommended in cytotoxicity testing, in order to avoid false positive results due to experimental artifacts. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 286-291, 1998.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 10
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    Long-term growth rates of four Mediterranean cold-water coral species maintained in aquaria (2011)
    Inter Research
    In:  Marine Ecology Progress Series, 429 . pp. 57-65.
    Details
    Publication Date: 2017-06-20
    Description: Growth rates of the cold-water corals (CWC) Madrepora oculata, Lophelia pertusa, Desmophyllum dianthus and Dendrophyllia cornigera were measured over 8 mo under controlled conditions (12°C in the dark, fed 5 times a week) by means of the buoyant weight technique. Additionally, linear growth rates were measured in M. oculata and L. pertusa for 2 and 1 yr, respectively. The weight measurements revealed growth rates, expressed as percent growth per day (mean ± SD), of 0.11 ± 0.04 for M. oculata, 0.02 ± 0.01 for L. pertusa, 0.06 ± 0.03 for D. dianthus and 0.04 ± 0.02 % d–1 for D. cornigera. Growth in M. oculata was significantly higher (p 〈 0.0001) than in the other 3 CWC species. For M. oculata and L. pertusa, also linear growth was recorded. These values (mean ± SD) were 0.014 ± 0.007 and 0.024 ± 0.018 mm d–1 for M. oculata and L. pertusa, respectively. This is the first study that compares the growth rates of 4 different CWC species under the same experimental conditions of water flow, temperature, salinity and food supply. These corals have different growth rates, both in terms of total weight increase and linear increase, and these growth rates can be related to interspecific physiological differences. Data on growth rates are essential to understand the population dynamics of CWC as well as the recovery capacity of these communities after disturbance.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.3354/meps09104
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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