Publication Date:
2024-03-15
Description:
Euryhaline decapod crustaceans possess an efficient regulation apparatus located in the gill epithelia, providing a high adaptation potential to varying environmental abiotic conditions. Even though many studies focussed on the osmoregulatory capacity of the gills, acid-base regulatory mechanisms have obtained much less attention. In the present study, underlying principles and effects of elevated pCO2 on acid-base regulatory patterns were investigated in the green crab Carcinus maenas acclimated to diluted seawater. In gill perfusion experiments, all investigated gills 4-9 were observed to up-regulate the pH of the hemolymph by 0.1-0.2 units. Anterior gills, especially gill 4, were identified to be most efficient in the equivalent proton excretion rate. Ammonia excretion rates mirrored this pattern among gills, indicating a linkage between both processes. In specimen exposed to elevated pCO2 levels for at least 7 days, mimicking a future ocean scenario as predicted until the year 2300, hemolymph K+ and ammonia concentrations were significantly elevated, and an increased ammonia excretion rate was observed. A detailed quantitative gene expression analysis revealed that upon elevated pCO2 exposure, mRNA levels of transcripts hypothesized to be involved in ammonia and acid-base regulation (Rhesus-like protein, membrane-bound carbonic anhydrase, Na+/K+-ATPase) were affected predominantly in the non-osmoregulating anterior gills.
Keywords:
Acid-base regulation; Alkalinity, total; Ammonia excretion; Ammonia excretion, relative; Ammonia excretion, relative, standard deviation; Ammonia excretion, standard deviation; Animalia; Aragonite saturation state; Arthropoda; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carcinus maenas; Containers and aquaria (20-1000 L or 〈 1 m**2); Coulometric titration; Decrease of haemolymph proton concentration; Decrease of haemolymph proton concentration, relative; Decrease of haemolymph proton concentration, relative, standard deviation; Decrease of haemolymph proton concentration, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Gene expression (incl. proteomics); Haemolymph, pH; Haemolymph, pH, standard deviation; Identification; Laboratory experiment; Laboratory strains; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Protein name; Salinity; Sample code/label; Single species; Species; Temperature, water; Treatment
Type:
Dataset
Format:
text/tab-separated-values, 3407 data points
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