ISSN:
1434-0879
Keywords:
Cholinergic nerves
;
Glandular cells
;
Nitric oxide
;
Secretion
;
Seminal vesicle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The significance of nitric oxide (NO) formation in seminal secretion was studied in guinea-pig seminal vesicles. The nitric oxide synthase (NOS) activity was estimated and reduced nicotinamide-adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry was performed. Furthermore, cyclic guanosine 3,5-monophosphate (cGMP) concentration as well as fructose secretion from isolated vesicles was estimated. High Ca2+-dependent NOS activity as well as prominent glandular NADPH-diaphorase staining was found in the secretory epithelium. The NOS inhibitorsN G-nitrol-arginine methyl ester (L-NAME) andN G-nitrol-ar-ginine (L-NNA) inhibited carbachol-induced fructose secretion but the D-isomer to L-NAME had no effect. Whenl-arginine was administered together with L-NAME, no inhibitory effect on the carbachol-induced fructose secretion could be seen. Nerve-induced fructose secretion was also inhibited by L-NAME. The NO donor glyceryl trinitrate (GTN) increased the fructose secretion. Carbachol or GTN did not increase cGMP levels, nor was fructose secretion inhibited by a guanylate cyclase inhibitor (ODQ). Our results suggests that glandular NO production is a prerequisite for muscarinic fructose secretion in the seminal vesicle via a cGMP-independent pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01268862
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