ISSN:
1573-5192
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary 1. The technique of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) has been evaluated as a method for the characterization of trypanosomes. 2. Twenty-five populations, including seven clones, isolated from bats in Europe, Canada and Latin America could be grouped into eight subpopulations having similar polypeptide profiles. We propose to designate such subpopulations by the term ‘peptideme’. 3. Our peptidemes were compared with the previous classification, obtained by DNA buoyant density (Newton, 1976), isoenzym electrophoresis and morphological studies (Baker et al., 1976, 1978; Baker & Miles, 1979) as follows: 4. Peptideme 1 corresponded with Trypanosoma dionisii dionisii and peptideme 2 with T. d. breve and T. Hedricki. However, peptidemes 1 and 2 had some common shared characters. 5. Peptideme 3 corresponded with T. myoti and peptideme 4 with strain Z of T. cruzi marinkellei. Again, peptidemes 3 and 4 had some shared characters and these also shared some characters with peptidemes 1 and 2. 6. Peptideme 5 contained strains N2 and N6 of T. vespertilionis; the other strains of this species (482 and 482 clone 1), although sharing some common characters, were sufficiently different to enable us to group them as peptideme 6. 7. The remaining T. c. marinkellei strains were grouped into peptidemes 7 (B7, M1116, M1117, M1117X) and 8 (B34, M1909), although once again, these two peptidemes had some shared characters. Thus the peptideme method of classification of these trypanosomes corresponds well with the classification proposed previously but it is a more sensitive method and can recognize more subtle variation between the strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00018999
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