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  • 1
    Electronic Resource
    Electronic Resource
    Thin-layer chromatography of red cell glycosphingolipids from various mammalian species
    Amsterdam : Elsevier
    Journal of Chromatography A 61 (1971), S. 285-293 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)92421-X
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  • 2
    Electronic Resource
    Electronic Resource
    Thin-layer chromatographic separation of gangliosides
    Amsterdam : Elsevier
    Journal of Chromatography A 106 (1975), S. 425-427 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)93856-1
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  • 3
    Electronic Resource
    Electronic Resource
    Separation of glycolipids from neutral lipids and phospholipids
    Amsterdam : Elsevier
    Journal of Chromatography A 153 (1978), S. 550-552 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)95521-3
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  • 4
    Electronic Resource
    Electronic Resource
    Parallel plastic tank experiments with cultures of marine diatoms (1977)
    Springer
    Helgoland marine research 30 (1977), S. 201-216 
    Details
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The reproductibility of tank experiments concerning unicellular marine algal development was analyzed by means of parallel experiments with cultures ofThalassiosira rotula andSkeletonema costatum, using large flexible plastic tanks under semi-natural conditions. The tanks (3–4 m3, 4–5 m deep) were exposed in the German Bight at a station in the outer harbour of helgoland. The water was obtained from the open North Sea in towable tanks; it was filtered (plate filter), enriched with nitrate (20–30 μgat dm−3), phosphate (1.3–2.3 μgat dm−3) and silicate (15–23 μgat dm−3)-nearly natural springtime concentrations in this area-and inoculated with 103–105 cells dm−3. The water was mixed with non-metal stirring equipment. Within 5 days, concentrations of 106–107 cells dm−3 in an exponential growth phase were obtained. In experiments withT. rotula a parallel development was achieved in spite of some contamination by surrounding water. This is the case for nearly all parameters analyzed (nutrient salts, phytoplankton, bacteria, C, N and particulate carbohydrates). The heterotrophic bacteria, which were determined by means of the plate method, reached concentrations of up to 106 (T. rotula) and 105 (S. costatum) CFU cm−3, respectively. They showed a consistent retrograde development at diatom concentrations above a certain level. The crop did not increase again until the diatoms had reached the stationary phase. During exponential growth ofT. rotula (G=8.9–11.7 h) a partially synchronous cell division was observed. There were also rhythms with respect to cell size (pervalvar axes) and chain length (number of cells). For the experiments withS. costatum (G=10–11.4 h) diurnal variations of cell size and chain length occurred. The present results indicate acceptable reproducibility of algal development and related phenomena in enclosed water bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02207836
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  • 5
    Electronic Resource
    Electronic Resource
    Einfache folientanks zur Planktonuntersuchung in situ (1974)
    Springer
    Marine biology 24 (1974), S. 163-166 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung 1. Für Planktonuntersuchungen in situ wurden einfache Tanks (ca. 4 m3, 5 m Tiefe) konstruiert, mit denen im Vorhafen von Helgoland während der Sommermonate 1972 und 1973 Versuche bis zu 28 Tagen durchgeführt wurden. 2. Lichtdurchlässige Kombinationsfolien aus Polyamid und Polyäthylen erwiesen sich für die an diesem Standort vorkommenden mechanischen Belastungen als geeignet. 3. Für eine kontinuierliche Durchmischung des Tankinhalts wurde ein Rührwerk entwickelt. Alle mit dem Tankinhalt in Berührung kommenden Teile waren aus physiologisch unbedenklichem Kunststoffmaterial gefertigt. 4. Die Tanks eignen sich für Simultanversuche, wie in Versuchen mit Monokulturen gezeigt werden konnte.
    Notes: Abstract For studies of in situ interactions between plankton and ambient water, simple, cylindrical, 5 m deep translucent-foil tanks were constructed using polyamide and polyethylene (layers 30 and 100 μm, respectively, with polyamide layer outside). The tanks were suspended in the water by metal frames fixed to a buoy. The whole construction was anchored in an outer basin of the harbour of Helgoland, N. Sea, and was exposed to tides and waves generated by winds of up to 6 Bft (Beaufort scale). Even under these conditions the construction and its material allowed experiments lasting 2 weeks and longer, during the summer months of 1972 and 1973. The banks' volume, of about 4 m3 each permits experiments with naturally occurring concentrations of nutrients and organisms and large sampling volumes for the determination of trace components. In this way, natural plankton populations in 1972, and monocultures of different diatom species in 1973 were investigated. Under these conditions, different monocultures developed almost identically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389351
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  • 6
    Electronic Resource
    Electronic Resource
    Dissolved organic substances during a Phaeocystis pouchetii bloom in the German Bight (North Sea) (1985)
    Springer
    Marine biology 89 (1985), S. 311-316 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dissolved organic substances (carbohydrates, organic nitrogen, free amino acids) were measured in the German Bight (North Sea) in June, 1981. During and before this survey, sea foam was observed in the east Frisian coastal water and it accumulated on the nearby beaches to an unusually high extent. In this coastal water area, a large Phaeocystis pouchetii Lagerheim bloom and very high concentrations of dissolved organic matter were found. The above dissolved organic substances were all positively correlated to a highly significant degree with P. pouchetii cell numbers in the bloom area. An influence of salinity (or river water) on this correlation could be excluded. Thus, exudation or decomposition products of P. pouchetii were most likely the cause of the unusually high concentrations of dissolved organic matter in the bloom area off the east Frisian coast, where P. pouchetii blooms have been reported for many years. Ammonia concentrations were very low in the P. pouchetii bloom area; this and the accumulation of dissolved organic substances might lead to speculation that decomposition of dissolved organic matter by bacteria could have been reduced due to antibiotic activity of P. pouchetii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393665
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  • 7
    Electronic Resource
    Electronic Resource
    Kinetics of osmoregulation in the crab Carcinus maenas (1972)
    Springer
    Marine biology 17 (1972), S. 291-303 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When shore crabs Carcinus maenas are transferred from 11 to 38‰ S at 11°C, new constant levels of hemolymph freezing points and of concentrations of Na, K, Ca, and Mg in the hemolymph are accomplished within 24h. From a decrease in serum protein and in serum free amino acids and an increase in the relative amounts of individual essential free amino acids in the body fluids of whole crabs, a participation of proteolytic activities and a transport of amino acids from hemolymph to cell is deduced; the stationary concentration of total intracellular free amino acids increases up to a nearly two-fold value, compared with the concentration in crabs remaining in diluted sea water. Also, the low molecular neutral sugars increase in whole crabs after high salinity stress, reaching values of more than two-fold initial concentrations within a period of 10 days. This increase is fully accounted for by a 6.7-fold increase in trehalose levels. The processes of increasing the concentrations of low molecular organic material seem to be slower than those of establishing new osmotic pressures in the hemolymph. The oxygen consumption decreases by 30 to 45% to new constant values within 8 to 12 h after the salinity change from 11 to 38‰, reflecting similar kinetics as the establishment of new osmotic pressures in the hemolymph.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00366739
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  • 8
    Electronic Resource
    Electronic Resource
    The development of a natural plankton population in an outdoor tank with nutrient-poor sea water. I. Phytoplankton succession (1977)
    Springer
    Marine biology 43 (1977), S. 1-17 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Due to complex hydrodynamic and biological inhomogeneities, the phytoplankton species succession cannot be satisfactorily observed, apart from the seasonal blooms which occur in temperate waters. Large flexible plastic tanks have proved to be useful for such observations. In 1972, for 28 days, a phytoplankton succession in nutrient-poor water in the outer harbor of Helgoland was observed in a flexible plastic tank (3 m3). During this period, 3 phytoplankton biomass maxima were formed with many significant correlations. In the first 7 days the ammonia concentration decreased from over 6 to 2 μ mol 1-1 for 14 days. The nitrate concentration remained in the range of 3–6 μ mol 1-1 and then fell abruptly to 0–2 μ mol 1-1. The phosphate concentration was about 0.1 μ mol 1-1. Lauderia borealis dominated the first period, and its increase was significantly correlated with the decrease in ammonia. The diatom was succeeded by two dinoflagellates, Dinophysis acuminata and Prorocentrum micans. The last period of the experiment was characterized by a stronger development of Rhizosolenia species. The rapid recovery of the crop in the nutrient-poor water points to intensive remineralization processes. The irregular occurrence of ammonia near the surface was correlated with the appearance of Noctiluca miliaris at this depth. It is expected that repetitions of this type of experiment will permit further explanations of statistical correlations which are not yet clear. As a first step, in order to test hypotheses, a correlation analysis was employed to eliminate the statistically non-significant correlations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392567
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  • 9
    Electronic Resource
    Electronic Resource
    Automatic determination of total carbohydrates in sea water (1980)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 301 (1980), S. 17-19 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Kohlenhydraten in Wasser ; Spektralphotometrie ; AutoAnalyzer, Meerwasser, Tryptophan-Schwefelsäure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The recommended method is based on the l-tryptophane/sulphuric acid reaction and has been adapted for a continuous flow system with the Technicon AutoAnalyzer. A supplementary run (cold run) is required with more than 10μmol/l of nitrate. The presence of acetone or formaldehyde must be avoided. Total carbohydrates can be determined by this method within the range of 0.02–5mg/l. The relative coefficient of variation at the 1 mg/l level is 0.25 % (glucose).
    Notes: Zusammenfassung Die empfohlene Methode basiert auf der l-Tryptophan-Schwefelsäure-Reaktion und wurde dem Durchflußsystem des Technicon AutoAnalyzers angepaßt. Bei einem Nitratgehalt von mehr als 10μmol/l ist zur Feststellung einer Korrektur ein zusätzlicher »kalter« Durchlauf notwendig. Aceton oder Formaldehyd dürfen nicht anwesend sein. Die Bestimmung kann im Bereich von 0.02–5 mg/l erfolgen und weist einen relativen Variationskoeffizienten von 0.25 % bei 1 mg/l auf (Glucose).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481265
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  • 10
    Electronic Resource
    Electronic Resource
    Automatic methods for the determination of total dissolved and particulate carbohydrates in the marine environment (1986)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 323 (1986), S. 47-49 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The methods recommended for the determination of total dissolved and particulate carbohydrates in the marine environment are based on the l-tryptophan/sulfuric acid reaction. Automatic measurements are performed with a Technicon AutoAnalyzer II. Glucose is used for the calibration procedure. Interfering nitrate and nitrite are also measured with a Technicon AutoAnalyzer II; the values are subtracted from the total reading of the carbohydrate analyzer after calibration. Particulate carbohydrates are measured after sulfuric acid digestion of the residue on glassfiber filters. Both methods permit total carbohydrates to be determined exactly in the concentration range from 0.1–30 μmol/l.
    Notes: Zusammenfassung Die für die Bestimmung gelösten und partikulären Gesamt-Kohlenhydrats in Seewasser empfohlenen Methoden basieren beide auf der l-TryptophanSchwefelsäure-Reaktion. Die Messungen erfolgen automatisch im Technicon AutoAnalyzer II. Zur Eichung wird Glucose benutzt. Störendes Nitrat und Nitrit werden ebenfalls mit einem Technicon AutoAnalyzer II gemessen; die entsprechenden Werte werden nach Eichung vom Gesamtsignal des Zuckeranalysators abgezogen. Partikuläre Kohlenhydrate werden nach Aufschluß des Rückstandes auf Glasfaserfiltern mit Schwefelsäure gemessen. Beide Methoden liefern genaue Ergebnisse im Bereich von 0.1–30 μmol/l.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00531130
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