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  • 1
    Electronic Resource
    Electronic Resource
    Sensitivity to plating of Escherichia coli cells expressing the cryA gene from Bacillus thuringiensis var. israelensis (1992)
    Springer
    Molecular genetics and genomics 232 (1992), S. 162-165 
    Details
    ISSN: 1617-4623
    Keywords: Bacillus thuringiensis var. israelensis ; Mosquito larvicidal δ-endotoxin ; T7 RNA polymerase ; cytA gene product ; Inhibition of DNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal δ-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene 1, under the control of lacP. Loss of colony-forming ability without substantial lysis, associated with immediate inhibition of DNA synthesis, was observed after induction of transformed cells. The cytA gene product may kill E. colicells by disrupting their chromosome replicating apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00299149
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    Paper (German National Licenses)
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  • 2
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    Population genetics and reproductive strategies of two Notostraca (Crustacea) species from winter ponds in Israel (2016)
    Taylor & Francis
    In:  Zoology in the Middle East, 62 (4). pp. 331-341.
    Details
    Publication Date: 2019-02-01
    Description: Fluorescent-amplified fragment length polymorphism (FAFLP) fingerprinting assay was used to compare the genetic diversity within and between tadpole shrimps (Notostraca) populations of Lepidurus apus (n=7) and Triops cancriformis (n=2) from rain pools in Israel. Each ephemeral water body has revealed a unique fingerprint pattern with an entailed genetic drift between nearby ponds. High similarity of genotypic diversity within each geographic area led to three clusters of water bodies, north, south and center of Israel. FAFLP assays on several newly hatched individuals of T. cancriformis revealed high identity amongst kin, as compared to L. apus where newly hatched from the same maternal source showed high diversity. Results indicate that T. cancriformis populations from Israel are probably parthenogenetic as indicated by clonal structures. The higher genetic variability in the L. apus populations and in laboratory-hatched specimens indicates the existence of sexual reproduction.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1080/09397140.2016.1257404
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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