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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of a chromosomal α-amylase gene from Bacillus stearothermophilus (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 77 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have cloned and sequenced a gene for a heat-stable α-amylase from a natural isolate of Bacillus stearothermophilus. Previously, it had been shown that B. stearothermophilus amylase genes may be harboured on indigenous plasmids. We have found that our isolate harbours the amylase gene only on the chromosome and not on its indigenous plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04360.x
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  • 2
    Electronic Resource
    Electronic Resource
    A new method for integration and stable DNA amplification in poorly transformable bacilli (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 125 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We have developed a strategy for the integration and stable amplification of DNA sequences in the chromosome of poorly transformable bacilli, which avoids the presence of a functional plasmid replication system in the integrated DNA. The parental vector for integration contains two plus origins of replication from pUB110 in the same orientation on a single plasmid. Due to the direct repeats, such plasmids produce two individual progeny vectors, one of which is dependent on the other for replication, as it lacks a functional rep gene. We have used such a progeny vector system to integrate and amplify DNA on the chromosome of Bacillus licheniformis, and show that the structure is stable in the absence of selective pressure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07343.x
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular genetic analysis of the pullalanase B gene of Bacillus acidopullulyticus (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 115 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A fragment from Bacillus acidopullulyticus strain 294-16 encoding a pullunase activity has been cloned into Bacillus subtilis. The nucleotide sequence of the 3972 base pairs (bp) fragment has been determined and shown to include only one complete open reading frame (ORF) of 863 codons. The deduced amino acid sequence of this ORF, denoted pulB, shows homology to a number of amylolytic enzymes. Primary and secondary structure analysis indicates that the central region of the protein forms the catalytic domain in a characteristics (β/α)8 barrel. Three carboxylic acid residues essential for catalysis were identified. Regions within the catalytic domain proposed to be involved in substrate binding have been identified by homology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06621.x
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  • 4
    Electronic Resource
    Electronic Resource
    On the industrial use of Bacillus licheniformis: a review (1994)
    Springer
    Applied microbiology and biotechnology 40 (1994), S. 595-598 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract. We document here that in those rare cases where disease has been related to Bacillus licheniformis, infection was associated with bypassing the normal biological protective barriers or severely debililated patients. No case suggests any invasive properties of this bacterium. B. licheniformis can therefore be considered non-pathogenic to humans in general. Food-borne illness caused by possible B. licheniformis toxins have been reported, but only in a very few cases and only in connection with consumption of inappropriately prepared food. Considerable experience concerning the industrial use of recombinant B. licheniformis strains has now accumulated and authorities in the United States, Europe and Japan have approved production with and products from recombinant B. licheniformis strains. We conclude that B. licheniformis is a safe host for the production of harmless, industrial products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050034
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  • 5
    Electronic Resource
    Electronic Resource
    On the safety of Bacillus subtilis and B. amyloliquefaciens: a review (1991)
    Springer
    Applied microbiology and biotechnology 36 (1991), S. 1-4 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164689
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  • 6
    Electronic Resource
    Electronic Resource
    On the safety of Aspergillus oryzae: a review (1992)
    Springer
    Applied microbiology and biotechnology 36 (1992), S. 569-572 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conclusions Invasive growth or systemic infections by A. oryzae in healthy humans have never been reported. In a few cases, however, isolates identified as A. oryzae have been recovered from debilitated patients. A. oryzae has therefore low pathogenic potential but may, like many other harmless microorganisms, grow in human tissue under exceptional circumstances. Allergic diseases primarily caused by A. oryzae have been reported in few cases, but probably presuppose both a sensitivity to allergenic reactions and a massive exposure to conidia by inhalation. A. oryzae does not produce aflatoxins or any other cancerogenic metabolites. The absence of significant levels of mycotoxins in industrial products is regularly checked. We therefore consider A. oryzae an excellent host for the safe production of harmless products by recombinant strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00183230
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  • 7
    Electronic Resource
    Electronic Resource
    On the industrial use of Bacillus licheniformis: a review (1994)
    Springer
    Applied microbiology and biotechnology 40 (1994), S. 595-598 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We document here that in those rare cases where disease has been related to Bacillus licheniformis, infection was associated with bypassing the normal biological protective barriers or severely debililated patients. No case suggests any invasive properties of this bacterium. B. licheniformis can therefore be considered non-pathogenic to humans in general. Food-borne illness caused by possible B. licheniformis toxins have been reported, but only in a very few cases and only in connection with consumption of inappropriately prepared food. Considerable experience concerning the industrial use of recombinant B. licheniformis strains has now accumulated and authorities in the United States, Europe and Japan have approved production with and products from recombinant B. licheniformis strains. We conclude that B. licheniformis is a safe host for the production of harmless, industrial products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173313
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  • 8
    Electronic Resource
    Electronic Resource
    Improved mapping of the tyrS locus in Escherichia coli (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 647-650 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A tyrosyl-tRNA synthetase mutant of Escherichia coli was isolated and the tyrS gene assigned a map position between man and pdxH at 36.0 min on the chromosome. The tyrS mutant grew badly on broth as did previously described tyrS mutants. This sensitivity to broth was suppressed by tyrR mutations. F-prime factors were found to complement the tyrS mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337873
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  • 9
    Electronic Resource
    Electronic Resource
    cur-1, a mutation affecting the phenotype of sup + strains of Escherichia coli (1980)
    Springer
    Molecular genetics and genomics 180 (1980), S. 425-428 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cur-1, a mutation found in many strains of the Y10 line of Escherichia coli K-12, but not previously described, maps at 27 min, close to galU. cur-1 causes mucoidity or uracil requirement at 29°C depending on the genetic background. These phenotypes are suppressed by amber codon suppressors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425858
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  • 10
    Electronic Resource
    Electronic Resource
    Variations in phenotype of relB mutants of Escherichia coli and the effect of pus and sup mutations (1980)
    Springer
    Molecular genetics and genomics 180 (1980), S. 429-437 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three independen relB mutants were studied. During amino acid starvation they all accumulate RNA and produce an inhibitor of in vitro protein synthesis; after starvation growth is retarded for hours. The mutants differ in the degree to which the common phenotype is expressed, but the characteristic thermolability of the inhibitor is the same. The phenotype of the relB mutants is accentuated by amber suppressors, and this effect is counteracted by a mutation, pus-1 that maps at 19.5 min near aroA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425859
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