ISSN:
1439-0426
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
The distribution and residue depletion of thiamphenicol (TAP) were studied in seabass (Dicentrarchus labrax L.) and seabream (Sparus aurata L.) reared in field conditions at temperatures of 20–28 °C. The drug was administered orally as medicated feed at the rate of 40 mg active ingredient (a.i.) kg−1 of biomass once a day for 5 days. Samples of muscle, liver, skin and vertebrae from 10 fishes were collected on the 2nd and 4th day of treatment and 1, 2, 3, 5, 7, 9, 12, 15, 18, 21, 24, 27 and 30 days after the last administration of the drug, and were stored at −20 °C. Quantitative analysis of TAP was performed by high performance liquid chromatography (HPLC) after liquid-liquid extraction; the quantification limits of the HPLC method were 0.02 μg/g for muscle, and 0.05–0.10 μg/g for the other tissues. In seabass, TAP concentrations during treatment were higher in liver and muscle than in skin and vertebrae, and rapidly decreased after the end of medication. Three days after treatment ceased, TAP was still detectable in liver (0.41 ± 0.23 μg/g), vertebrae (0.09 ± 0.03 μg/g) and in three out of 10 samples of muscle (0.03 μg/g), but not in skin. All tissues were below the limits of quantification on the 5th day of withdrawal. In seabream the highest TAP concentrations during treatment were measured in liver and skin, and their reduction after the end of medication was as rapid as that of seabass: on the 3rd day after treatment ceased traces were found in only four out of 10 samples of muscle (0.03 ± 0.00 μg/g) and vertebrae (0.08 ± 0.02 μg/g).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1439-0426.2002.00305.x
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