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  • 1
    Electronic Resource
    Electronic Resource
    Acidic fog-induced changes in host-plant suitability (1989)
    Springer
    Journal of chemical ecology 15 (1989), S. 2379-2390 
    Details
    ISSN: 1573-1561
    Keywords: Acidic fog ; Phaseolus lunatus ; Trichoplusia ni ; cabbage looper ; Lepidoptera ; Noctuidae ; nitrogen ; free amino acid ; soluble protein ; plantinsect interactions ; air pollution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Phaseolus lunatus L. (Henderson Bush lima beans) were exposed to 2 hr acidic fogs with 2.5∶1.0 (v/v) nitrogen-sulfur ratio typical of the west coast of the United States. Fogs with pH values of 2.0 (P 〈 0.01,t tests), 2.5 (P 〈 0.05), or 3.0 (P 〈 0.01) increased percent total nitrogen (dry weight) of foliage as compared to plants subjected to control fogs with a pH of 6.3–6.5. Fresh weight concentrations of soluble protein and certain free amino acid concentrations were increased by plant exposure to acidic fogs with a pH of 2.5 (t tests,P 〈 0.05). Concentrations of free amino acids considered essential for insect growth, as well as nonessential and total free amino acids were not significantly affected by any treatment (P 〉 0.05,t test). Water content (%) of foliage was not changed significantly (P 〉 0.05,t test) by exposure to any of the fogs.Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) larvae ate significantly more foliage and gained significantly more weight on plants treated with 3.0 pH fogs (P 〈 0.01,t test). Several potential explanations are offered for the lack of significant weight gain by larvae on plants in which soluble protein levels, free amino acid concentrations, or percent total nitrogen contents were enhanced by acidic fogs with a pH of 2.5 and 2.0. No larval feeding preference was detected for foliage exposed to acidic versus control fogs, and no significant differences were detected in percent survival ofT. ni eggs exposed to acidic or control fogs. Some implications of acidic fogs for population dynamics ofT. ni are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012089
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  • 2
    Electronic Resource
    Electronic Resource
    Phthalide-based host-plant resistance toSpodoptera exigua andTrichoplusia ni inApium graveolens (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 709-726 
    Details
    ISSN: 1573-1561
    Keywords: Host-plant resistance ; phthalide ; sedanenolide ; celeriac ; Bacillus thuringiensis ; tritrophic interaction ; beet armyworm ; cabbage looper ; Apium graveolens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A chemical basis for the difference in suitability between two celeriac (Apium graveolens var.rapaceum) cultivars for the survival and growth ofSpodoptera exigua (Hübner) andTrichoplusia ni (Hübner) was identified as sedanenolide (3-n-butyl-4,5-dihydro-isobenzofuranone). Sedanenolide was isolated using a bioassay-driven extraction and purification procedure and was identified using several spectrometric methods. Foliar concentrations of sedanenolide were negatively correlated with larval performance and were significantly higher in the cultivar less suitable for larval survival and growth. Sedanenolide andBacillus thuringiensis Berliner acted additively in reducing larval growth when combined in artificial diets, a result that is consistent with previous studies in which the combined effect of host plant cultivar andB. thuringiensis on larval survival and growth were additive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02059608
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  • 3
    Electronic Resource
    Electronic Resource
    Ozone-induced changes in host-plant suitability: Interactions ofKeiferia lycopersicella andLycopersicon esculentum (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 203-218 
    Details
    ISSN: 1573-1561
    Keywords: Ozone ; nutrition ; insect-plant interactions ; nitrogen ; secondary plant compounds ; Keiferia lycopersicella Lepidoptera ; Gellechiidae ; tomato ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Tomato pinworms,Keiferia lycopersicella (Walsingham), survived better and developed faster on tomato plants,Lycopersicon esculentum Mill., damaged by ozone than on plants not subjected to ozone fumigation. Other measures of fitness, including survival during pupation, sex ratio of adults, female longevity, and fecundity, were not affected. Analyses of ozonated foliage at zero, two, and seven days following fumigation demonstrated a transient but significant increase (18–24%) in soluble protein concentration. Although the concentration of the total free amino acids in ozonated foliage did not increase significantly, significant changes were observed in at least 10 specific amino acids, some of which are critical for either insect development or the production of plant defensive chemicals. A reduction in total nitrogen in ozonated foliage at seven days postfumigation indicated that nitrogen was being translocated to other portions of the plant. The implications of increases in assimilable forms of nitrogen in ozonated foliage, which lead to improved host-plant suitability for insect herbivores, are discussed both in relation to some current ecological theories and in regard to pest-management strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01020363
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  • 4
    Electronic Resource
    Electronic Resource
    Measuring plant protein with the Bradford assay (1989)
    Springer
    Journal of chemical ecology 15 (1989), S. 979-992 
    Details
    ISSN: 1573-1561
    Keywords: Soluble protein ; Coomassie brilliant blue G-250 ; binding ; leaves ; extraction ; spectrophotometry ; protein assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-μl aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagent (Coomassie brilliant blue G-250) diluted 1:4 and containing 3 mg/ml soluble polyvinylpyrollidone. Absorbance at 595 nm is recorded after 15 min against an NaOH blank. Samples are calibrated against a ribulose 1,5-diphosphate carboxylase-oxygenase standard in NaOH. Procedures for plant preparation, extraction stability, the effects of phenol removal and quinone formation, and assay recovery are evaluated. Assay absorbance stability and techniques for increasing absorbance stability are reported. Changes in protein quality are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01015193
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    Paper (German National Licenses)
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