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  • 1
    Electronic Resource
    Electronic Resource
    Somatic Cell Genetics of Higher Plants (1974)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 8 (1974), S. 267-278 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.08.120174.001411
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  • 2
    Electronic Resource
    Electronic Resource
    Factors influencing the frequency of callus formation among cultured rice (Oryza sativa) anthers (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 51 (1981), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of various experimental parameters upon the frequency of callus formation from cultured anthers of Oryza sativa has been investigated. Although certain medium components were found to be critical to callus formation, the concentration of these components had little effect upon the frequency of callus formation. The degree to which the callus formation frequency was influenced by cold pretreatment of the flowers was variable. Even though plants were grown under uniform conditions and flowers containing pollen in the microspore stage of development were selected for dissection, the frequency of callus formation varied nonrandomly between flowers. In experiments with populations of flowers in a physiological and developmental state favorable to callus formation 35% of plated anthers produced callus and at least 60% of these calluses gave rise to green plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1981.tb02699.x
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  • 3
    Electronic Resource
    Electronic Resource
    Forced association between higher plant and bacterial cells in vitro (1974)
    [s.l.] : Nature Publishing Group
    Nature 252 (1974), S. 393-395 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To form the Azotobacter plant cell association, carrot cell suspensions growing logarithmically in a liquid medium8 containing standard nitrogen levels were inoculated with cells of an adenine-requiring strain of A. vinelandii to a final concentration of 106ml-1 bacterial cells. After 12 d the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/252393a0
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  • 4
    Electronic Resource
    Electronic Resource
    A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 177-182 
    Details
    ISSN: 1432-2242
    Keywords: Herbicide resistance ; Acetolactate synthase ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cultures of Nicotiana tabacum cells homozgous for a mutation (S4) at the SuRB locus that confers resistance to the sulfonylurea herbicides chlorsulfuron and sulfometuron methyl (Chaleff and Ray 1984; Chaleff and Bascomb 1987) were used to isolate a doubly mutant cell line (S4 Hra/S4+) resistant to even higher herbicide concentrations. Growth of cells homozygous for both the S4 and Hra mutations (S4 Hra/S4 Hra) was uninhibited by a herbicide concentration 500-fold higher than a concentration by which growth of S4+/S4+ callus was inhibited by 75%. Plants homozygous for both mutations were at least five-fold more resistant to foliar applications of chlorsulfuron than were singly mutant S4+/S4+ plants. This enhanced resistance was inherited as a single, semidominant, nuclear trait that is genetically linked to the S4 mutation. Acetolactate synthase (ALS) activity in extracts of leaves of doubly mutant (S4 Hra/S4 Hra) plants was approximately 20-fold more resistant to inhibition by chlorsulfuron and sulfometuron methyl than was ALS activity in singly mutant (S4+/ S4+) leaf extracts, which was in turn more resistant to inhibition by these compounds than was the normal enzyme. Extracts prepared from plants of these three genotypes possessed the same ALS specific activities. Therefore, Hra represents a second independent mutation at or near the SuRB locus that reduces the sensitivity of tobacco ALS activity to inhibition by sulfonylurea herbicides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257843
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  • 5
    Electronic Resource
    Electronic Resource
    Further characterization of picloram-tolerant mutants of Nicotiana tabacum (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 91-95 
    Details
    ISSN: 1432-2242
    Keywords: Cell culture ; Picloram-tolerance ; Genetics ; Uptake studies ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A genetic and preliminary biochemical analysis has been performed on four picloram-tolerant mutants of Nicotiana tabacum that were isolated from cell cultures. The four mutations define three distinct linkage groups. Mutant seedlings incorporate radioactively labeled picloram normally and do not modify or degrade the herbicide in a manner that alters its solubility characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277772
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  • 6
    Electronic Resource
    Electronic Resource
    Induction, maintenance, and differentiation of rice callus cultures on ammonium as sole nitrogen source (1982)
    Springer
    Plant cell, tissue and organ culture 2 (1982), S. 29-37 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rice callus was initiated from cultured anthers and was maintained on medium containing ammonium as sole nitrogen source and supplemented with either a buffer or an organic acid. Plant regeneration also was obtained on this medium, but the morphogenetic capacity of the callus culture declined as rapidly as on a medium containing a mixture of ammonium and nitrate. The results of the studies suggest that organic acids provide an additional function beyond that of buffering the medium during growth on ammonium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033550
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic and biochemical evidence for multiple forms of acetolactate synthase in Nicotiana tabacum (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 33-38 
    Details
    ISSN: 1617-4623
    Keywords: Sulfonylurea herbicides ; Resistance ; Acetolactate synthase ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutations conferring resistance to sulfonylurea herbicides have been identified at two unlinked genetic loci (SuRA and SuRB) in Nicotiana tabacum. A mutation (S4) residing at the SuRB locus causes production of an altered form of acetolactate synthase (ALS) that is insensitive to inhibition by sulfonylurea herbicides and that constitutes approximately 50% of total ALS activity. Another mutation (C3) at the SuRA locus similarly results in herbicide resistance of approximately half of the total ALS activity. These results suggest that N. tabacum possesses at least two isozymic forms of ALS. The relative and absolute amounts of the isozymes are affected by a mutation that segregates independently of C3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337755
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic and physiological variability among cultured cells and regenerated plants of Nicotiana tabacum (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 254-258 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tobacco cell lines selected for resistance to picloram (4-amino-3,5,6-trichloropicolinic acid) and plants regenerated from these cell lines manifest several traits not shown by the parental strains. Genetics analyses of the regenerated plants have permitted the sources of this variability to be identified. Tricotyledenous seedlings appeared at a much higher frequency among the progeny of a heterozygous mutant plant (PmR1/+) regenerated from culture than they did among progeny of normal regenerated plants. In crosses with the regenerated heterozygous mutant plant and with homozygous progeny of this plant (PmR1/PmR1) the frequency of tricotyly was influenced more by the generation than by the genotype of the parent plant. Therefore, it is concluded that tricotyly is a physiological response to passage through cell culture. More than half of the picloram-resistant cell lines isolated were also resistant to hydroxyurea. Segregation of these two resistances was analyzed in progeny of crosses with regenerated plants. In all cases hydroxyurea-resistance was genetically stable and inherited as a single dominant nuclear mutation (designated HuR). In crosses with plants PmR1/+ and PmR7/+ the HuR and PmR mutations assorted independently. In contrast, the HuR mutation recovered from plant PmR6/+ was linked to the PmR6 mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268434
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic characterization of hydroxyurea-resistant mutants obtained from cell cultures of Nicotiana tabacum (1983)
    Springer
    Molecular genetics and genomics 192 (1983), S. 218-224 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic characterization of seven hydroxyurearesistant (HuR) mutants selected from cell cultures of Nicotiana tabacum var. Xanthi has shown that in each case resistance is inherited as a single, dominant, nuclear mutation. Four HuR mutants for which linkage analysis has been performed identify two unlinked loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327669
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