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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two molecular marker approaches [amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR)] were employed to study genomic relationship among 96 rice cultivars. These included most of the best reputed Italian accessions. AFLP produced 461 fragments, 248 (53%) of which were polymorphic, SSR produced four to 11 alleles in the 12 genomic loci investigated. Genomic similarity was estimated independently for the two molecular marker techniques. Both AFLP and SSR dendrograms agree in splitting the cultivars into two main clusters: a small one, comprising four exotic accessions, and a larger one which could be split into four subgroups. These were also analysed on the basis of historical and pedigree information. This is the first report on the application of DNA polymorphism analysis to reveal genomic relationship among cultivated Italian rice germplasm. Results will be useful for breeding programmes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0168-9452
    Keywords: Asparagus officinalis ; dioecy ; sex
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Surveys in geophysics 16 (1995), S. 227-252 
    ISSN: 1573-0956
    Keywords: Flash-flood ; Rainfall-runoff modelling ; Multi-sensor approach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In the study of flash-flood occurrence in small catchments the lack of flow measurements is often one of the main limiting factors. Prior to estimating the forecasting potentialities and techniques for such events, an accurate reconstruction of past event flood dynamics is first required. This issue is here addressed by analyzing, with the use of a distributed hydrological model, the hydrometeorological conditions in which a severe flash-flood occurred, on October 1992, on a 48 square kilometers catchment in the Arno basin. Such an event was caused by the persistence of intense convective clusters on the background of widespread rain bands of frontal origin. The distributed hydrological model here adopted is devoted to simulate the evolution and the variability of the primary processes involved in the runoff cycle. Together with the hydrological model structure, other particular aspects of the event reconstruction procedure are discussed: the managing and processing of the information coming from different sensors, with different temporal and spatial resolutions; the identification of local precipitation dynamics (frontal or convective) within small areas of integrated radar and rain gauges data fields; the interpolation of rain gauge data on the basis of the radar-estimated spatial correlation. The results of the distributed modeling, concerning the estimate of the flood wave at various sites, are compared with analogous results obtained with simpler lumped models.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2145
    Keywords: Asparagus ; Flower development ; Sex differentiation ; Male and female RNase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Asparagus officinalis is a dioecious plant. The flowers start to develop as hermaphrodites and later become unisexual. In female flowers the stamens degenerate, while in male flowers the ovary stops growing without degenerating. We have examined young asparagus flowers using SEM and optical microscopy in order to determine the exact moment of transition from hermaphroditic to unisexual development. We defined 13 stages of development, starting from flower primordia up to completely mature flowers and labelled them with numbers from -6 to 7. The first five stages are fully hermaphroditic: a difference between sexes becomes visible at stage — 1 when the style begins to develop in female flowers. Degeneration of stamens in female flowers starts somewhat later. At the stage of transition, some differences between sexes also appear in the bidimensional polypeptide pattern of flowers. RNase activity shows a distinct peak at this stage (in female flowers only), probably related to stamen degeneration.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2242
    Keywords: Key words Asparagus ; Dioecious species ; Genetic map ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome 5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Asparagus officinalis L ; Genetic map RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A preliminary genetic map of the dioecious species Asparagus officinalis L. (2n = 20) has been constructed on the basis of restriction fragment length polymorphism (RFLP) and isozyme marker data. With DNA samples digested with either EcoRI or HindIII 61 out of 148 probes (41%) identified RFLPs in six families of doubled haploid lines obtained through anther culture. A higher level of polymorphism (65%) was observed when a single family was screened for RFLPs using six distinct restriction enzymes. Segregation analysis of the BC progenies (40–80 individuals) resulted in a 418-cM extended map comprising 43 markers: 39 RFLPs, three isozymes and one morphological (sex). These markers are clustered in 12 linkage groups and four of them exhibited significant deviations from the expected 1∶1 ratio. One isozyme and three RFLP markers were assigned to the sex chromosome.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 212 (1998), S. 177-184 
    ISSN: 1615-6110
    Keywords: Orchidaceae ; Ophrys bertolonii agg. ; O. sphegodes ; O. fuciflora ; Molecular (RAPD) analysis ; Flora of Italy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The RAPD technique has been used for analyzing the genetic material of seven Italian populations of theOphrys bertolonii aggregate (Orchidaceae). For comparison the related speciesO. sphegodes andO. fuciflora have also been analyzed. A high genetic variability has been observed within the same populations, and on the whole onlyO. bertolonii can be safely differentiated from the other taxa of the aggregate, which are undistinguishable with this method.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-0956
    Keywords: Meteorology ; Radar ; Raingauge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In this paper, some considerations are given to the employment of C-band polarimetric weather radars for rainfall estimates. The most common error sources are discussed, such as ground clutter and propagation attenuation effects, together with decorrelation in the sampling at the ground between radar and raingauge measurements, which can be quite significant in radar systems located in hilly regions, as is the case of the Arno basin in Tuscany. Since the main objective from a hydrological point of view is the estimate of rainfall at ground, integrations and comparisons are needed between radar and raingauge data, which are characterized by different time and space sampling. The paper is then focussed mainly on this problem and a technique is presented in order to improve radar based rainfall estimates through the integration with raingauge data, in order to enhance the correlation between the two types of measurements. Such a method is finally applied to a serious meteorological event which affected the Arno basin on October 1992.
    Type of Medium: Electronic Resource
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