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  • 1
    Book
    Book
    Heidelberg [u.a.] : Spektrum, Akad. Verl.
    Keywords: Genetics ; Genetics, Medical ; Molecular Biology ; Lehrbuch Handbuch Standardwerk ; genetics ; Einführung ; Genetik ; Genetik
    Type of Medium: Book
    Pages: XXIII, 493 S. , Ill., graph. Darst.
    ISBN: 3860251805
    Uniform Title: Genetics: a molecular approach 〈dt.〉
    RVK:
    RVK:
    Language: German
    Note: Literaturangaben
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 77 (1991), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pseudomonas syringae 1105 was grown under a variety of culture conditions and the effects on ice nucleation determined. Growth in a defined medium, Koser citrate broth, resulted in a greater number of more active ice nuclei than growth in either of three complex media. The ice nucleation phenotype was not expressed uniformly during the growth cycle in broth culture. Instead a maximal level of ice nucleation was observed during the middle to exponential phase and the phenotype was not expressed in stationary phase. Cells grown on agar plates expressed the phenotype to a much greater extent than cells from broth cultures.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0983
    Keywords: Allomyces macrogynus ; mtDNA ; Restriction mapping ; Gene localization ; mtDNA instability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A physical map of the mitochondrial genome of the aquatic phycomycete Allomyces macrogynus strain Burma 3–35 (35°C) has previously been published (Borkhardt and Delius 1983). This map has been extended in this study by locating 37 additional recognition sites for five new restriction enzymes in the mitochondrial genome. Homologous regions for the genes coding for cytochrome oxidase subunits 1, 2, and 3, apocytochrome b, ATPase subunits 6 and 9, the small and large ribosomal RNA, URF1, URF5, and perhaps urfa, a presumptive gene hitherto found only in the mitochondrial genome of the fission yeast Schizosaccharomyces pombe, were located in the mitochondrial genome of A. macrogynus by heterologous hybridizations with specific, mitochondria) gene probes from Saccharomyces cerevisiae, Aspergillus nidulans, Neurospora crassa, and S. pombe. The mitochondrial gene order in A. macrogynus was found to be identical to that of A. arbuscula; a gene order hitherto found only among members of the family Blastocladiaceae. Spontaneous insertion mutations have been found to occur quite frequently in the mitochondrial genome of A. macrogynus. In all mutated mitochondrial genomes so far studied, insertions have been located in a specific region located between the genes coding for the ATPase subunit 9 and the large ribosomal RNA. In two of the mutated mitochondrial genomes the insertional event(s) resulted in the presence of mitochondrial DNA molecules differing in size by multiples of approximately 70 base pairs.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 300 (1982), S. 719-724 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] On the basis of available nucleotide sequence and genetic data, we present a model for RNA splicing in fungal mitochondria. Seven intron RNAs of two fungal species can form identical secondary structures, involving four conserved sequences, which bring the ends of each intron together and allow an ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-203X
    Keywords: Acetosyringone ; Agrobacterium tumefaciens ; tomato ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Explants of tomato (Lycopersicon esculentum Mill cv. Ailsa Craig) were co-cultivated with Agrobacterium tumefaciens C58C1Rifr::pGSFR1161 in the presence of 20 (μM acetosyringone). Transformed root clones were selected on kanamycin medium and the presence of the nptII gene in the plant DNA confirmed by the polymerase chain reaction. Root clones derived from acetosyringone treatment grew more vigorously in the presence of kanamycin and synthesized a greater amount of NPT-II enzyme. The conclusion is that acetosyringone treatment enhances the transformation process, possibly by stimulating multiple insertions of the T-DNA into the host genome.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0983
    Keywords: Aspergillus nidulans ; DNA sequencing ; Mitochondrial DNA ; ATPase subunit 9
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of a 74 codon reading frame from the Aspergillus nidulans mitochondrial genome is presented. The derived amino acid sequence displays typical features of dicyclohexylcarbodiimide (DCCD) binding proteins and is 84% homologous with a mitochondrial reading frame that potentially encodes an ATPase subunit 9 polypeptide in Neurospora crassa. However, in A. nidulans, as in N. crassa, there is strong biochemical and genetic evidence that this subunit is in fact nuclearly-encoded. In both organisms the DCCD-binding protein found in the F0 complexes of mitochondria from actively-growing cultures is almost certainly the product of this nuclear gene, and definitely not that of the mitochondrial reading frame. The discovery of an intact open reading frame than can code for a DCCD-binding protein in the mitochondrial genome of a second species of filamentous fungus strenghthens the possibility that the presence of a mitochondrial version of this gene has some biological significance.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Nucleic acid extracts from 1400-year-old radish seeds recovered from excavations at Qasr Ibrim, Upper Egypt, have been analysed by gas chromatography/mass spectrometry and gas chromatography/mass spectrometry/mass spectrometry. tert-Butyldimethyisilyl derivatives of the purine and pyrimidine bases were prepared, after treatment of the nucleic acid extracts with concentrated formic acid. Under electron ionization these derivatives yield prominent [M-57]+ ions that were found to be of value for use in analyses employing selected-ion monitoring and product-ion studies. These two techniques were used in the sensitive and selective detection of nucieotide bases in crude extracts of ancient seeds. The results obtained were supported by complementary analyses of hydrolysates of ancient nucleic acids and authentic bases by means of retention time and spectral comparisons. This work demonstrates for the first time that mass spectrometry can be used in the direct chemical examination of nucleotide bases in ancient materials. This analytical approach is currently being used to address questions regarding the possible chemical (diagenetic) changes occurring in the nucleic acids of ancient biological material.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    BioEssays 16 (1994), S. 719-726 
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ancient DNA has been discovered in many types of preserved biological material, including bones, mummies, museum skins, insects in amber and plant fossils, and has become an important research tool in disciplines as diverse as archaeology, conservation biology and forensic science. In archaeology, ancient DNA can contribute both to the interpretation of individual sites and to the development of hypotheses about past populations. Site interpretation is aided by DNA-based sex typing of fragmentary human bones, and by the use of genetic techniques to assess the degree of kinship between the remains of different individuals. On a broader scale, population migrations can be traced by studying genetic markers in ancient DNA, as in recent studies of the colonisation of the Pacific islands, while ancient DNA in preserved plant remains can provide information on the development of agriculture.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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