Note: Intro -- Preface -- Contents -- Chapter 1: Evaluation of Quality and Safety of Foods by Tandem Mass Spectrometry -- 1.1 Introduction -- 1.2 Results and Discussion -- 1.2.1 Determination of Oleuropein in Olive Oil -- 1.2.2 Assessment of Resveratrol Amount in Red Wine -- 1.2.3 Determination of Phenylethanoids in Extra Virgin Olive Oil -- 1.2.4 Application of PS-MSMS for the Determination of Quality Markers in Foodstuff -- 1.3 Conclusions -- References -- Chapter 2: Detection of Biological Warfare Agents Using Biosensors -- 2.1 Introduction -- 2.1.1 Classification of Biological Agents -- 2.1.2 Analytical Measurement of Biological Agents -- 2.2 What Is the Definition of Biosensors? -- 2.3 Overview of Predominant Sensing Techniques -- 2.3.1 Pathogenic Bacteria Electrical Detection Via Immobilized Antimicrobial Peptides -- 2.3.1.1 Development of an AMP-Based, Label-Free Electronic Biosensor -- 2.3.1.2 Real-Time Detection of Pathogenic Bacteria -- 2.3.1.3 Selectivity Measurements -- 2.3.2 NRL Array Biosensor for Toxin Detection -- 2.3.2.1 Toxins Environmental Testing in Food and Air -- 2.3.2.2 Use of Antimicrobial Peptides for Toxin Detection -- 2.3.3 Surface Acoustic Wave (SAW) Sensors -- 2.3.4 Biosensing with Luminescent Semiconductor Quantum Dots -- 2.3.4.1 Immunoassays Using Quantum Dots -- 2.3.4.2 Nucleic Acid Detection -- 2.3.4.3 Sensing Based on FRET with Quantum Dot Bioconjugates -- 2.3.5 Engineered Cell-Based Sensors: The CANARY System -- 2.3.5.1 CANARY Bioelectronic Sensor -- 2.3.5.2 Toxin Detection -- 2.3.5.3 Detection of the DNA Sequence -- 2.3.5.4 CANARY Detections of Francisella tularensis and Yersinia pestis -- 2.3.6 High-Density Microsphere-Based Fiber-Optic DNA Microarrays -- 2.3.6.1 Fiber Optic DNA Biosensors -- 2.3.6.2 Analysis Setup and Protocol -- 2.3.7 Surface Plasmon Resonance -- 2.3.7.1 SPR Principle. , 2.3.7.2 High-Throughput Screening (HTS) -- 2.3.7.3 Surface Plasmon Resonance Sensing of Biological Warfare Agent Botulinum Neurotoxin A -- 2.3.7.3.1 Immobilization of BoNT/A Antibody and SV Protein on CM5 SPR Sensor Chip -- 2.4 Conclusion -- References -- Chapter 3: Mass Spectrometry Methods for Food Safety/Detection of Toxins in Food -- 3.1 Mass Spectrometry: An Overview -- 3.2 Ionization Techniques -- 3.2.1 Volatile Molecules -- 3.2.2 Polar Analytes -- 3.3 Analyzers -- 3.4 Study of Complex Mixtures by MS -- 3.5 Identification, Confirmation and Quantitation of Analytes by Mass Spectrometry -- 3.5.1 Structural Characterization and Identification of Unknowns: Untarget Analysis -- 3.5.1.1 Data Dependent Acquisition (DDA) -- 3.5.1.2 Data Independent Acquisition (DIA) -- 3.5.2 Confirm Presence/Absence of Known Analytes and Quantitation: Target Analysis -- 3.6 Food Safety/Detection of Toxins in Food -- References -- Chapter 4: Fundamentals of Mass Spectrometry-Based Metabolomics -- 4.1 Introduction -- 4.1.1 Targeted Vs. Untargeted Assays -- 4.2 Sample Preparation -- 4.3 Separations -- 4.3.1 Liquid Chromatography -- 4.3.2 Gas Chromatography -- 4.3.3 Capillary Electrophoresis -- 4.3.4 Ion Mobility -- 4.4 Mass Spectrometry -- 4.5 Data Analysis -- 4.6 Other Approaches to Metabolomics -- 4.6.1 Combining Metabolomics with Other Omics Technolgies -- 4.6.2 Metabolomic Analysis with Imaging Mass Spectrometry -- 4.6.3 Other Metabolomics Methods: Nuclear Magnetic Resonance -- 4.7 Conclusion -- References -- Chapter 5: Direct Mass Spectrometry as a Practical Analytical Strategy for High Speed, High Throughput Testing -- 5.1 Introduction -- 5.2 Direct Analysis MS Approaches as an Alternative to GC/LC Combined with Tandem Mass Spectrometry -- 5.3 Direct MS Applications with an Emphasis on DART Applications. , 5.4 Special Consideration in Quantitative Applications by DART and DESI -- 5.5 Conclusions -- References -- Chapter 6: Mass Spectrometry in Ecotoxicology -- 6.1 Evolution of Environmental Analytical Tools -- 6.1.1 Analyzing the "Universe of Chemicals" -- 6.1.2 Effect-Directed Analysis (EDA) -- 6.2 Mass Spectrometry for Characterizing Interactions of Chemicals with Organisms -- 6.3 Conclusions -- References -- Chapter 7: Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry: Technology and Applications -- 7.1 Introduction -- 7.2 Spatial Resolution Improvements in IMS -- 7.3 Increasing Ion Intensity for High Spatial Resolution IMS -- 7.4 Unravelling Molecular Complexity Using IMS -- 7.5 Sample Preparation for IMS -- 7.6 MALDI Matrix Selection and Application -- 7.7 Application to Kidney Disease -- 7.8 Applications to Infectious Disease -- 7.9 Data Analysis with IMS -- 7.10 Data Import, Pre-Processing, and Visualization -- 7.11 Statistical and Multivariate Analysis -- 7.12 Common Software for IMS Data Analysis -- 7.13 Data Registration and Multimodal Approaches -- 7.14 Conclusion -- References -- Chapter 8: Risks and Consequences of Hazard Agents to Human Health -- 8.1 Human Health and Disease -- 8.2 Environmental Disasters Impacting Human Health -- 8.3 Environmental Health -- 8.4 One Health and Global Health -- 8.5 Classification of Health Hazard Agents -- 8.5.1 Physical Health Hazard Agents -- 8.5.2 Chemical Health Hazard Agents -- 8.5.3 Biological Health Hazard Agents -- 8.6 Biohazards and Bioterrorism -- 8.7 Risk Assessment of Health Hazards -- 8.7.1 Identification Methods for Biological Warfare Health Hazards -- 8.8 Concluding Remarks -- References -- Chapter 9: Bacterial Threats to Human Health and Food Supply -- 9.1 Introduction -- 9.2 Food-borne Pathogens -- 9.3 Biothreat Biological Bacterial Agents. , 9.4 Bacterial Toxins at the Host/Bacterium Interface -- References -- Chapter 10: An Overview of Male Reproductive Toxicants: Facts and Opinions -- 10.1 Introduction -- 10.2 The Testicular Function -- 10.2.1 The Cycle of the Seminiferous Epithelium -- 10.2.2 The Sertoli Cell-Germ Cell Communication System -- 10.2.3 Germ Cell Activity -- 10.2.4 The Peritubular Cell-Seminiferous Epithelium Communication System -- 10.2.5 The Sertoli Cell-Leydig Cell Communication System -- 10.2.6 Regulation of Spermatogenesis -- 10.3 The Epididymal Function -- 10.4 Toxicologic Evaluation of Environmental Chemicals on the Reproductive Sphere -- 10.4.1 Endocrine Disruptors -- 10.4.2 The Testicular Dysgenesis Syndrome -- 10.5 Evaluation of the Male Reproductive Toxicity of a Substance -- 10.5.1 Understanding the REACH Regulation -- 10.5.2 Tools for Male Reproductive Toxicity Evaluation -- 10.5.3 Key Characteristics of Male Reproductive Toxicants -- 10.5.4 Imaging Mass Spectrometry: A Powerful Complementary Tool for Understanding Toxicity Mechanisms -- 10.6 Conclusion -- References -- Chapter 11: Defense Against Biological Terrorism: Vaccines and Their Characterizations -- 11.1 Classification of Diseases Caused by Biological Terrorism -- 11.1.1 Old Infective Diseases -- 11.1.1.1 Smallpox (Variola) -- 11.1.2 Existing Active Infective Diseases -- 11.1.2.1 Anthrax (Carbuncle) -- 11.1.2.2 Plague (Pestis, Black Death) -- 11.1.2.3 Tetanus -- 11.1.3 New Infective Diseases -- 11.1.3.1 Ebola/Marburg Diseases -- 11.1.3.2 Influenza Virus, with New Strains, Continuously Appearing -- 11.1.4 New Virus Causing World Pandemic -- 11.1.5 General Considerations -- 11.2 Biothreats and Risk Level Assessment -- 11.2.1 Category A Agents -- 11.2.2 Category B Agents -- 11.2.3 Category C Agents -- 11.3 Immunology: Origins and Development -- 11.4 Assessing Degrees of Mucosal Involvement. , 11.5 Prevention to Favor Defense and Immunity Success -- 11.5.1 Actions Against Germs -- 11.6 Preparation of Vaccines -- 11.6.1 Requirements for Vaccine Preparation -- 11.6.2 Different Method of Vaccinations -- 11.6.3 Vaccination Campaigns: How Best Use Vaccines -- 11.7 Development of Biothreat Vaccines -- 11.7.1 Glycoconjugate Vaccines Used for Prevention from Biological Agents -- 11.7.2 Lipopolysaccharide (LPS) -- 11.7.3 LPS-Derived Vaccines or LPS-Protein Neoglycoconjugates -- 11.7.4 Molecular Weight and Carbohydrate-to-Protein Ratio Determination -- 11.7.5 Mass Spectrometry Methods for the Characterization of Carbohydrate Vaccines -- 11.7.6 MALDI-TOF-MS -- 11.7.7 SELDI-TOF-MS -- 11.7.8 Glycation Sites Determination -- 11.8 A Typical Example of the Mass Spectrometry Characterization of an Anthrax Biothreat -- 11.8.1 Carbohydrate: BSA Ratio -- 11.8.2 Glycation Sites Determination -- 11.8.2.1 MALDI-TOF/TOF-MS/MS -- 11.8.2.2 LC-MS/MS -- 11.9 Conclusion -- References -- Chapter 12: Environmental Impacts of Air Pollution on Human Health in Annaba Region (Northeast of Algeria) -- 12.1 Introduction -- 12.2 Study Area -- 12.3 Limit Values for Parameters of Atmospheric Emissions in Algeria -- 12.4 Air Quality Monitoring in Annaba Region -- 12.5 Health Impact of Pollution in Annaba -- 12.6 Correlation Between Particulate Matter Concentrations and the Number of Asthma Crisis -- 12.7 Relation Between Dust Concentrations and the Number of Other Respiratory Emergencies -- 12.8 Conclusions -- References -- Chapter 13: New Insight into Idiopathic Nephrotic Syndrome: Strategy Based on Urinary Exosomes -- References -- Chapter 14: Synthesis of Long-Chain Esters Under Continuous Flow Conditions -- References -- Chapter 15: Data Independent Acquisition Mass Spectrometry for Proteomic Advances into Isolated Methylmalonic Acidemia -- References. , Chapter 16: Deposition Strategies of Nano-TiO2 Photocatalyst for Wastewater Applications.

Note: Front cover -- Contents -- Preface -- Editors -- Contributors -- Chapter 1. Overview of Recent Developments in the Mass Spectrometry of Nucleic Acid and Constituents -- Chapter 2. Mass Spectrometric Analysis of Deoxyinosines -- Chapter 3. Tandem Mass Spectrometry of Nucleic Acids -- Chapter 4. Mass Spectrometric Application in the Study of Cyclic Nucleotides in Biochemical Signal Transduction -- Chapter 5. Analysis of Urinary Modified Nucleosides by Mass Spectrometry -- Chapter 6. Mass Spectrometric Determination of DNA Adducts in Human Carcinogenesis -- Chapter 7. Sequence Distribution of Nucleobase Adducts Studied by Isotope Labeling of DNA-Mass Spectrometry -- Chapter 8. Electrospray Mass Spectrometry of Noncovalent Complexes between Small Molecule Ligands and Nucleic Acids -- Chapter 9. Electrospray Ionization-Mass Spectrometry for the Investigation of Protein- Nucleic Acid Interactions -- Chapter 10. Characterization of Noncovalent Complexes of Nucleic Acids with Peptides and Proteins by Mass Spectrometry -- Chapter 11. MALDI-TOF Detection of Specific Noncovalent Complexes of Highly Acidic Biomolecules with Pyrenemethylguanidinium -- Chapter 12. Quantitative Identification of Nucleic Acids via Signature Digestion Products Detected Using Mass Spectrometry -- Chapter 13. Electrospray Ionization Mass Spectrometry for the Direct Analysis of Modified Nucleosides in Small RNAs -- Chapter 14. LC-MS/MS for the Examination of the Cytotoxic and Mutagenic Properties of DNA Lesions In Vitro and In Vivo -- Index -- Back cover.

Note: Intro -- Preface -- Contents -- Chapter 1: Immunorecognition of Biological Agents: An Introduction to Immunology -- 1.1 Introduction -- 1.2 Microbes and Humans Interacting: Defense Strategies of Multicellular Live Beings -- 1.3 Immunology: Origins and Development of a Still Young Discipline -- 1.4 Microbes and Their Attack Strategies -- 1.5 Prevention to Favour Defence and Immunity -- References -- Chapter 2: Vaccines, Sera and "New" Viruses: Ebola, Zika and Other Infectious Challenges for Human Health -- 2.1 Vaccines and Sera -- 2.2 Main Diseases of Interest in the Field -- 2.3 Definitions and History of Bioterrorism -- 2.4 Main Infectious Agents in Bioterrorism and New Infections -- 2.4.1 Smallpox (Variola) -- 2.4.2 Poliomyelitis (Infantile Paralysis) -- 2.4.3 Anthrax (Carbuncle) -- 2.4.4 Plague (Pestis, Black Death) -- 2.4.5 Tularemia -- 2.4.6 Tetanus -- 2.4.7 Botulism -- 2.4.8 Tuberculosis (TBC) -- 2.4.9 Severe Acute Respiratory Syndrome (SARS) -- 2.4.10 Ebola/Marburg Diseases -- 2.4.11 Hantavirus, Lassa Fever, etc -- 2.4.12 Zika Virus Disease -- 2.4.13 Influenza Virus, with New Strains Continuously Appearing -- 2.5 Conclusions -- References -- Chapter 3: Fundamental Principles for Sensing Measuring Devices Used for the Detection of Chemical Warfare Agents -- 3.1 Introduction -- 3.2 Various Types of Chemical Agents -- 3.2.1 Cyanides -- 3.2.2 Nerve Agents -- 3.2.3 Toxic Industrial Chemicals -- 3.2.4 Blood Agents -- 3.2.5 Blister Agents -- 3.2.6 Psychotomimetic Chemical Weapons -- 3.2.7 Riot Control Agents -- 3.3 Analytical Chemical Sensors -- 3.3.1 Sensor Classification -- 3.3.2 Sensor Selection -- 3.3.3 Factors to Consider When Selecting Sensors -- 3.4 Technological Requirements for the Separation and Detection of Warfare CAs -- 3.5 Available Detection Technologies -- 3.5.1 Flame Photometric Detector Technology -- 3.5.2 Infra-red Sensors. , 3.5.2.1 Photoacoustic Infra-red Spectroscopy (PIRS) -- 3.5.2.2 Filter Infra-red Detectors (the Interferometer) -- 3.5.2.3 Forward-Looking Infra-red Spectroscopy (FLIR) -- 3.5.2.4 Long Wave Infrared (LWIR) Absorption Spectroscopy or Hyperspectral Imaging -- 3.5.2.5 Release of Sulfur Hexafluoride (SF6) from Five Different Locations Recorded from a Drone -- 3.6 Raman Sensors -- 3.6.1 Differential Absorption Light Detection (LIDAR) -- 3.6.1.1 Radiative Transfer Sensors (RTS) -- 3.6.2 LaserScan Detection System -- 3.7 Ion Mobility-Mass Spectrometry or Ion Mobility Spectrometry (IMS) -- 3.7.1 Drift Tubes Ion Mobility Spectrometry (DT-IMS) -- 3.7.2 Aspiration Ion Mobility Spectrometry (AIMS) -- 3.7.3 Travelling-Wave Ion Mobility Spectrometry (TWIMS) -- 3.7.4 High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) -- 3.8 Color-Change Chemistry -- 3.8.1 Dräger Tubes -- 3.9 Photo Ionization Detectors (PIDs) -- 3.10 Conclusion -- References -- Chapter 4: Fundamental Principles for Luminescence Sensing Measuring Devices Used for the Detection of Biological Warfare Agents -- 4.1 Introduction -- 4.1.1 Classification of Biological Agents -- 4.2 Analytical Measurement of Biological Agents -- 4.2.1 What Are Biosensors? -- 4.2.2 Overview of Predominant Sensing Techniques -- 4.3 Electrical Detection of Pathogenic Bacteria via Immobilized Antimicrobial Peptides -- 4.3.1 Development of an AMP-Based, Label-Free Electronic Biosensor -- 4.3.2 Pathogenic Bacteria Real-Time Detection -- 4.3.3 Selectivity Measurements -- 4.4 NRL Array Biosensor for Toxin Detection -- 4.4.1 Toxins Environmental Testing in Food and in Air -- 4.4.2 Use of Antimicrobial Peptides for Toxin Detection -- 4.5 Surface Acoustic Wave (SAW) Sensors -- 4.6 Biosensing with Luminescent Semiconductor Quantum Dots -- 4.6.1 Immunoassays Using Quantum Dots -- 4.6.2 Nucleic Acid Detection. , 4.6.3 Sensing Based on FRET with Quantum Dot Bioconjugates -- 4.7 Engineered Cell-Based Sensors: The CANARY System -- 4.7.1 CANARY Bioelectronic Sensor -- 4.7.2 Toxin Detection -- 4.7.3 DNA Sequence Detection -- 4.7.4 CANARY Detection of Francisella tularensis and Yersinia pestis -- 4.8 High-Density Microsphere-Based Fiber Optic DNA Microarrays -- 4.8.1 Fiber Optic DNA Biosensors -- 4.8.2 Analysis Setup and Protocol -- 4.9 Surface Plasmon Resonance -- 4.9.1 SPR Principle -- 4.9.2 High-Throughput Screening (HTS) -- 4.9.3 Surface Plasmon Resonance Sensing of Biological Warfare Agent Botulinum Neurotoxin A -- 4.9.3.1 Immobilization of BoNT/A Antibody and SV Protein on CM5 SPR Sensor Chip -- 4.10 Conclusion -- References -- Chapter 5: Mass Spectrometry and High Resolution Mass Spectrometry: An Overview -- 5.1 Mass Spectrometry: An Overview -- 5.2 Ionization Techniques -- 5.3 Analyzers -- 5.4 Resolving Power, Resolution and Accurate Mass Measurements -- 5.5 Tandem Mass Spectrometry -- 5.6 Conclusion -- References -- Chapter 6: Primary Considerations in Quantitative Mass Spectrometry -- 6.1 Introduction -- 6.1.1 Overview -- 6.1.2 The Concept of Fitness for Purpose -- 6.1.3 Why Quantify? -- 6.1.4 Approaches to Quantification -- 6.1.5 The Unique Benefits of MS Analysis -- 6.1.6 Types of Quantitative Analysis -- 6.2 The Practice of Quantitative Chemical Analysis -- 6.2.1 Introduction -- 6.2.2 General Strategies for Quantitative Analysis -- 6.3 Key Terms -- 6.4 Additional Considerations in Quantitative Analysis -- 6.4.1 Analyte, Sample and SampleCollection Issues -- 6.4.2 The Internal Standard -- 6.4.3 Extraction and Sample Work Up -- 6.4.4 Documentation -- 6.5 Quality Control Practices & -- Method Validation -- 6.6 Conclusions -- References -- Chapter 7: Imaging Mass Spectrometry - Molecular Microscopy for Biological and Clinical Research. , 7.1 Introduction -- 7.2 Background/History -- 7.3 MALDI Imaging Mass Spectrometry: Technology and Protocols -- 7.4 Molecular Identification -- 7.5 Applications to Diabetes Research -- 7.6 Applications to the Diagnosis of Melanoma -- 7.7 Fusion of IMS and Microscopy Images -- 7.8 Conclusion and Perspective -- References -- Chapter 8: MALDI Mass Spectrometry and Infectious Diseases -- 8.1 Introduction -- 8.2 MALDI Mass Spectrometry and Infectious Diseases -- 8.3 Diagnostic Microbiology -- 8.4 MALDI Profiling of Infected Human Tissues -- 8.5 Imaging Mass Spectrometry -- 8.5.1 MALDI-IMS -- 8.5.2 Staphylococcus aureus Infections -- 8.5.3 Analyte Identification Strategies -- 8.5.4 Protein Identification Strategies -- 8.6 Peptide Identification Strategies -- 8.7 Conclusions -- References -- Chapter 9: Soft X-ray Radiation Applied in the Analysis of Intact Viruses and Antibodies by Means of Nano Electrospray Differential Mobility Analysis -- 9.1 Introduction -- 9.2 Experimental -- 9.2.1 Instrumentation -- 9.2.2 Samples -- 9.3 Results and Discussion -- 9.4 Conclusions -- References -- Chapter 10: Mass Spectrometry in Environmental Chemistry and Toxicology -- 10.1 Introduction -- 10.2 The Universe of Chemicals -- 10.3 Effect-Directed Analysis -- 10.4 Mass Spectrometry for Characterizing Interactions of Chemicals with Organisms -- 10.5 Conclusions -- References -- Chapter 11: Enhancing the Analysis of Complex Lipid Samples Through Developments in Chromatography and Chemical Derivatization -- 11.1 Lipidomics, Lipid Structure and Function -- 11.2 Lipid Extraction, Separation and Analysis -- 11.2.1 MS Analysis of Lipid Samples -- 11.2.2 Optimization of Lipid Chromatography -- 11.3 Chemical Derivatization to Improve Sensitivity in Lipidomics Analyses -- 11.3.1 Fatty Acyl Derivatization -- 11.3.2 Di-, and Triacylglycerol Derivatization. , 11.3.3 PE and PC Derivatization -- 11.4 Conclusion -- References -- Chapter 12: Advanced Mass Spectrometric Methodologies in the Evaluation of Health Risk Assessment Associated to Exposure to Drugs and Fraudulenty Modified Foods -- 12.1 The Absolute Method of Analysis -- 12.2 Quality Safety and Tracebility of Foods [7] -- 12.2.1 Babies Injured by Melamine Fraudulently Added in Milk -- 12.2.2 Cancerogenic Sudan Dyes Molecules Added to Sausages -- 12.2.3 PS/MS Discrimination Among Citrus Essential Oils -- 12.2.4 Analysis of Sexual Assault Evidence -- 12.2.5 Identification of Minor Components in Food by LTP Mass Spectrometry -- 12.2.6 Clinical Applications of Ambient Ionization Procedures -- 12.3 Conclusion -- References -- Chapter 13: A Tandem Mass Spectrometry Strategy for Validating the Synthesis of Glycoconjugate Vaccines -- 13.1 Introduction -- 13.2 Methods for Neoglycoconjugates Synthesis -- 13.3 Role of the Biomolecule Protein Carrier -- 13.4 Role of the Antigenic Carbohydrate Hapten in the Glycoconjugate Vaccine -- 13.5 Role of Antigenic Haptens Conjugates Against Cancer -- 13.6 Role of Mass Spectrometry in Validation of Glycoconjugate Vaccines Synthesis -- 13.6.1 Strategies for the Characterization of Glycoconjugate Vaccines Using MS -- 13.6.2 Molecular Mass and Carbohydrate-to-Protein Ratio Determination -- 13.6.3 Glycation Sites Determination -- 13.6.3.1 Neoglycoconjugate Vaccine of the Biological Agent Bacillus anthracis, the Etiological Agent of Anthrax -- 13.6.3.2 Anti-tumor Thomsen-Friedenreich Neoglycoconjugate Vaccine Prepared by Michael Addition -- 13.7 Conclusion -- References -- Chapter 14: Explosive Detection Strategies for Security Screening at Airports -- 14.1 Introduction -- 14.2 Security Technologies to Detect Explosives Contained Within Objects. , 14.3 Security Technologies to Detect Explosives Contained on the Surface of Objects.