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  • 1
    Electronic Resource
    Electronic Resource
    Comprehensive metabolic profiling and phenotyping of interspecific introgression lines for tomato improvement (2006)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 24 (2006), S. 447-454 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Tomato represents an important source of fiber and nutrients in the human diet and is a central model for the study of fruit biology. To identify components of fruit metabolic composition, here we have phenotyped tomato introgression lines (ILs) containing chromosome segments of a wild species in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1192
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight (2000)
    Springer
    Planta 211 (2000), S. 841-845 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Arabidopsis (PHB production) – Metabolite profiling (quadrupole mass spectrometry) – Polyhydroxyalkanoate – Polyhydroxybutyrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract.  Transgenic Arabidopsis thaliana (L.) Heynh. plants expressing the three enzymes encoding the biosynthetic route to polyhydroxybutyrate (PHB) are described. These plants accumulated more than 4% of their fresh weight (≈40% of their dry weight) in the form of PHB in leaf chloroplasts. These very high producers were obtained and identified following a novel strategy consisting of a rapid GC-MS analysis of a large number of transgenic Arabidopsis plants generated using a triple construct, thus allowing the parallel transfer of all three genes necessary for PHB synthesis in a single transformation event. The level of PHB produced was 4-fold greater than previously published values, thus demonstrating the large potential of plants to produce this renewable resource. However, the high levels of the polymer produced had severe effects on both plant development and metabolism. Stunted growth and a loss of fertility were observed in the high-producing lines. Analysis of the metabolite composition of these lines using a GC-MS method that we have newly developed showed that the accumulation of high levels of PHB was not accompanied by an appreciable change in either the composition or the amount of fatty acids. Substantial changes were, however, observed in the levels of various organic acids, amino acids, sugars and sugar alcohols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250000350
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  • 3
    Electronic Resource
    Electronic Resource
    Resistance in tomato to Xanthomonas campestris pv vesicatoria is determined by alleles of the pepper-specific avirulence gene avrBs3 (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 261-269 
    Details
    ISSN: 1617-4623
    Keywords: Avirulence gene ; avrBs3 ; Disease resistance ; Tomato ; Xanthomonas campestris pv vesicatoria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacterial spot disease of tomato and pepper caused by Xanthomonas campestris pv vesicatoria is prevented by resistance genes in the plant that match genes for avirulence in the bacterium. Based on DNA homology to the avirulence gene avrBs3, which induces the resistance response on pepper, we have isolated another avirulence gene from X. c. vesicatoria, designated avrBs3-2. This gene differs in specificity from avrBs3 in inducing the hypersensitive response on tomato but not on pepper. Sequence analysis of the avrBs3-2 gene revealed a high degree of conservation: the 3480 by open reading frame contains an internal region of 17.5 nearly identical 102 bp repeat units that differ in their order from those present in the avrBs3 gene. The coding region is 97% identical to avrBs3 and expresses constitutively a 122 kDa protein, thus representing a natural allele of this gene. The previously isolated 1.7 kb avrBsP gene from X. c. vesicatoria is 100% identical to the corresponding avrBs3-2 sequence, indicating that these genes might be identical. Interestingly, derivatives of avrBs3-2 lacking the C-terminal region and part of the repetitive region are still able to confer incompatibility in tomato. The avrBs3-2 gene is compared with the sequence of avrBs3 derivatives generated by deletion of repeat units that also have avirulence activity on tomato. Both genes, avrBs3 and avrBs3-2, are flanked by a 62 by long inverted repeat, which prompts speculations about the origin of the members of the avrBs3 gene family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279555
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