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  • 1
    Electronic Resource
    Electronic Resource
    Construction of a yeast plasmid cloning vector with high stability inSaccharomyces cerevisiae strains deficient in 2 µmDNA (1984)
    Springer
    Monatshefte für Chemie 115 (1984), S. 1229-1235 
    Details
    ISSN: 1434-4475
    Keywords: Cloning vectors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung PlasmidpCP1 wurde ausgehend von klonierterSaccharomyces cerevisiae-2 µmDNA und PlasmidpJDB207 konstruiert. Der Vektor enthält die vollständige, imFLP-Gen unterbrocheneB-Form der 2 µmDNA, vomEscherichia coli-Plasmidp AT153 abgeleiteteDNA und eine wenig aktive Variante desS. cerevisiae-LEU2-Gens. Der neue Vektor weist unter nichtselektiven Wachstumsbedingungen incir +- undcir 0-Stämmen eine niedrige Verlustrate auf und ist incir 0-Stämmen stabil gegen Umlagerungen. Seine Verwendbarkeit für die Umwandlung voncir +-Stämmen incir 0-Stämme durch Verdrängung endogener 2 µm-DNA wurde nachgewiesen.
    Notes: Abstract PlasmidpCP1 was constructed from cloned 2 µmDNA ofSaccharomyces cerevisiae and from plasmidpJDB207. VectorpCP1 contains the completeB form of 2 µmDNA interrupted in theFLP gene, together withDNA derived from theEscherichia coli plasmidpAT153 and a low expression variant of theS. cerevisiae LEU2 gene. The new vector is lost at a low frequency from yeastcir + orcir 0 strains under non-selective growth conditions and is stable against rearrangements incir 0 strains. Its usefulness for curingcir + strains from endogenous 2 µmDNA and for their conversion tocir 0 strains was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00809354
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  • 2
    Electronic Resource
    Electronic Resource
    Intraspecific length heterogeneity of the rDNA-IGR in Arabidopsis thaliana due to homologous recombination (1993)
    Springer
    Plant molecular biology 22 (1993), S. 543-545 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; rDNA ; intergenic region ; spacer heterogeneity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called ‘C’ repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the observed length heterogeneity is due to homologous recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015983
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  • 3
    Electronic Resource
    Electronic Resource
    ITS sequences from nuclear rDNA suggest unexpected phylogenetic relationships between Euro-Mediterranean, East Asiatic and North American taxa ofQuercus (Fagaceae) (1998)
    Springer
    Plant systematics and evolution 211 (1998), S. 129-139 
    Details
    ISSN: 1615-6110
    Keywords: Fagaceae ; Castanea ; Quercus ; Fagus ; Nuclear rDNA ; ITS ; molecular phylogeny ; molecular systematics ; phytogeography ; N Hemisphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nucleotide sequences from the internal transcribed spacer (ITS) regions of the 18S–26S nuclear ribosomal DNA have been studied from ten species ofQuercus (representing four subgenera),Castanea sativa andFagus sylvatica, as a preliminary molecular contribution to the still poorly understood systematics and evolution ofFagaceae. The resulting matrix has been used to calculate pair-wise sequence divergence indices and to construct a maximum parsimony tree forQuercus coding indels as a fifth state. Divergence is greater forQuercus vs.Fagus than forQuercus vs.Castanea. The tree for theQuercus taxa studied reveals two clearly divergent clades. In clade I the evergreen W MediterraneanQ. suber appears in a basal position as sister to more distal deciduous taxa, i.e. the E MediterraneanQ. macrolepis and the E AsiaticQ. acutissima (all formerly united as different sections under the apparently polyphyletic subg.Cerris), andQ. rubra (a representative of the N American subg.Erythrobalanus), forming a pair withQ. acutissima. In clade II the evergreen southeastern N AmericanQ. virginiana is basal and sister to the remaining three branches, i.e. a pair of evergreen Mediterranean taxa withQ. ilex andQ. coccifera (subg.Sclerophyllodrys), the deciduous but otherwise plesiomorphic SE European/SW AsiaticQ. cerris (type species of subg.Cerris), and the related but more apomorphic European pairQ. petraea andQ. robur (subg.Quercus). These results partly conflict with current taxonomic classification but are supported by some anatomical and morphological characters. They document polyphyletic lines from evergreen to deciduous taxa and suggest Tertiary transcontinental connections within the genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984917
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  • 4
    Electronic Resource
    Electronic Resource
    The Tpv2 family of retrotransposons of Phaseolus vulgaris: structure, integration characteristics, and use for genotype classification (1999)
    Springer
    Plant molecular biology 39 (1999), S. 797-807 
    Details
    ISSN: 1573-5028
    Keywords: common bean cultivars ; copia/Ty1 type retrotransposons ; Phaseolus vulgaris ; transposon insertion sites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Tpv2 family of transposable elements of common bean, Phaseolus vulgaris, belongs to the Ty1/copia group of long terminal repeat (LTR) containing retrotransposons. By reverse transcriptase (RT)-PCR and by analysis of genomic clones, we characterized four of the approximately 40 copies present in the Phaseolus genome, and the genomic environment of their integration sites. Tpv2 integrated preferentially into actively transcribed regions. While none of the isolated elements had all the functional domains necessary for transposition, analysis of bean cultivars suggested that some members of the Tpv2 family transposed in recent breeding history. Probes derived from Tpv2, as well as flanking genomic sequences, may be useful for classifying Phaseolus cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006132532684
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