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  • 1
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Growth and survival of the scallop Lyropecten nodosus were studied in 1997 at two sites (inner and outer Turpialito Bay) during a non-upwelling period normally occurring between August and November. Individuals had an initial shell height of 4.86 cm (SD=1.64 cm). Both experimental groups were held in suspended plastic baskets at the same depth (4 m). Measurements of shell height and dry weights of shell, gonad, digestive gland, remaining tissues and shell biofouling were taken at monthly intervals. Environmental parameters, including temperature, phytoplanktonic biomass, total particulate material (TPM) and associated organic (POM) and inorganic (PIM) fractions, were recorded simultaneously. At the end of the study, significant differences in growth and survival of scallops were observed between the two experimental sites. Scallops maintained inside the bay showed a 22% greater increase in shell height (7.41±0.27 cm) than those placed outside the bay (6.37±0.41 cm). Survival of scallops inside the bay was 31% higher compared with scallops outside. The greater availability of food of phytoplanktonic origin during the first two experimental months (July and August) together with greater POM throughout the whole experimental period except September, at the inner bay site, probably explained survival and growth differences observed between the two locations. Results suggest that, during the non-upwelling period (characterized by low primary productivity and high water temperatures), POM of sedimentary origin may play an important role as an energy source required for metabolic and reproductive activities of L. nodosus.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isochrysis galbana clone T-ISO was cultured in 5-L vessels with continuous light at 100 µmol photons m−2 s−1 at temperature room (22 °C) and harvested at early exponential phase. Dry weight (dw), ash content, biochemical composition and fatty acid profile were determined in fresh biomass and after storage for 7 days and 3 months at −76 °C for both freeze-dried and frozen biomasses. Both freezing and freeze-drying caused significant losses in dry weight (range 13.67–19.74%) when compared with fresh microalgae (40.17 pg cell−1), irrespective of storage time. Ash content increased significantly (P 〈 0.05) from 21.15% in the case of fresh T-ISO up to a range of 24.13–26.26% when different treatments were used. Both freezing and freeze-drying techniques caused a significant protein loss of a similar magnitude from 13.60 to 11.74 and 11.40 pg organic weight (OW) cell−1, respectively, only when long-term storage was used (3 months). Carbohydrate content was only affected by the preservation technique (P 〈 0.01). Freeze-drying caused significant loss of the latter component after both 7 days (4.53 pg OW cell−1) and 3 months (4.24 pg OW cell−1) when compared with fresh T-ISO (6.15 pg OW cell−1). Lipid content remained constant when subjected to either microalgal treatment or storage time (P 〉 0.05). However, the relative percentage of polyunsaturated fatty acids underwent a significant loss after 3 months when T-ISO was freeze-dried. Our results suggest that freezing is a more appropriate long-term preservation technique (3 months) than freeze-drying. Both carbohydrate and polyunsaturated fatty acids contents, important components in the determination of the nutritional value of food in aquaculture (i.e. for bivalves), remain unchanged when frozen. The processes responsible for the observed loss of biochemical compounds when samples are freeze-dried is most probably as a result of a physical altering of the cell wall.
    Type of Medium: Electronic Resource
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