ISSN:
1432-0843
Keywords:
Oocytes
;
Xenopus laevis
;
Transport P-glycoprotein
;
Renal
;
Deoxytubercidin Vinblastine
;
mRNA
;
Translation
;
Nucleoside
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The hypothesis that P-glycoprotein (P-gp) mediates the renal secretion of organic cations was tested by functional expression of mRNAs in theXenopus laevis oocyte system. Efflux of 2′-deoxytubercidin (dTub), a substrate for the renal organic cation transporter (OCT) but not for P-gp, was enhanced by injection of renal mRNA but not by injection of mRNA from P-gp-overexpressing cells (MDCK cells transduced with the cDNA for humanMDR1). The functional capacity of the MDCK-MDR mRNA was established by its ability to reduce, the steady-state uptake of a classical P-gp substrate, vinblastine. Thus, these data indicate OCT and P-gp to be distinct entities. TheXenopus oocyte system provides a functional approach to further characterize the OCT.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00685648
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