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  • 1
    Electronic Resource
    Electronic Resource
    Multiple facets of bacterial porins (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 199 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Porins form channels allowing the transport of molecules across lipid bilayer membranes. Their structure, location and large number on the bacterial surface lend them multiple functions. Porin loops are potential targets for adhesion to other cells and binding of bactericidal compounds to the surface of Gram-negative bacteria. Variation of the loop structure as a mechanism to escape immune pressure, or modulation of the porin expression in response to the presence of antibiotics, are survival strategies developed by some pathogenic bacteria. Porins may play a significant role as pathogenesis effectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10642.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Impact of crop management on intraspecific diversity of Pseudomonas corrugata in bulk soil (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 31 (2000), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The genetic structure of Pseudomonas corrugata populations was investigated in bulk soil to evaluate the impact of crop management on the intraspecific diversity of this bacterium stimulated in plant rhizosphere. As these bacteria are rare in bulk soil, an immunotrapping assay was developed to isolate them from soils located in Grignon (France), where adjacent plots with similar soil features were cultivated under maize/wheat crop rotation or continuous wheat cropping. Genomic fingerprinting of 291 isolates was performed using repetitive sequence-based polymerase chain reaction (ERIC-PCR). Most of these isolates (236 out of 291) were assigned to P. corrugata on the basis of an amplified ribosomal DNA restriction analysis (ARDRA) with 12 restriction enzymes. Data were analyzed by Pearson's chi-squared tests and genetic diversity was evaluated with specific indices for richness and evenness. Comparison of richness or evenness diversity indices (global diversity) showed no significant difference, whereas ERIC-PCR fingerprinting data (intraspecific diversity) showed that the genetic structure of P. corrugata was significantly affected by crop management.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2000.tb00666.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    DNA Extraction from Activated Sludges (1999)
    Springer
    Current microbiology 38 (1999), S. 315-319 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exchange resin was more efficient than sonication. The cell lysis procedures were applied in two sequences, and DNA was quantified after each cell lysis treatment. Lysozyme + SDS was the most effective step in the cell lysis procedures. The cell lysis treatment sequences giving the highest DNA yields were not the same for all the sludges. The differences in sludge microbial compositions and floc structures required specifically adapted cell lysis protocols. The proposed protocols were highly efficient for DNA extraction, yielding about 50 mg DNA g−1 volatile suspended solids, and allowed PCR amplification of 16S rDNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006809
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    Paper (German National Licenses)
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