In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 112, No. 27 ( 2015-07-07)
Abstract:
Klebsiella pneumoniae is now recognized as an urgent threat to human health because of the emergence of multidrug-resistant strains associated with hospital outbreaks and hypervirulent strains associated with severe community-acquired infections. K . pneumoniae is ubiquitous in the environment and can colonize and infect both plants and animals. However, little is known about the population structure of K . pneumoniae , so it is difficult to recognize or understand the emergence of clinically important clones within this highly genetically diverse species. Here we present a detailed genomic framework for K . pneumoniae based on whole-genome sequencing of more than 300 human and animal isolates spanning four continents. Our data provide genome-wide support for the splitting of K . pneumoniae into three distinct species, KpI ( K . pneumoniae ), KpII ( K . quasipneumoniae ), and KpIII ( K . variicola ). Further, for K . pneumoniae (KpI), the entity most frequently associated with human infection, we show the existence of 〉 150 deeply branching lineages including numerous multidrug-resistant or hypervirulent clones. We show K . pneumoniae has a large accessory genome approaching 30,000 protein-coding genes, including a number of virulence functions that are significantly associated with invasive community-acquired disease in humans. In our dataset, antimicrobial resistance genes were common among human carriage isolates and hospital-acquired infections, which generally lacked the genes associated with invasive disease. The convergence of virulence and resistance genes potentially could lead to the emergence of untreatable invasive K . pneumoniae infections; our data provide the whole-genome framework against which to track the emergence of such threats.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.1501049112
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2015
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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