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  • 1
    In: Molecules, MDPI AG, Vol. 27, No. 24 ( 2022-12-19), p. 9046-
    Abstract: The incidence of colon cancer is increasing year over year, seriously affecting human health and quality of life in recent years. However, traditional Chinese medicine (TCM) has been utilized for the treatment of colon cancer. S. officinalis Saponins (S-Saponins), the potential compound of TCM, displays multiple biological activities in colon cancer treatment. In our study, ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) combined with multivariate statistical analysis were performed to analyze and identify raw and processed saponins. Then, MTT and cell migration assays were used to preliminarily explore the effects of saponins in vitro on colon cancer cells. The results showed that 29 differential saponins compounds under Paozhi were identified by UHPLC-MS/MS. Moreover, in vitro validation showed that Sprocessed better inhibited the proliferation and migration of colon cancer cells than Sraw. This study provides a basis for the determination of the chemical fundamentals of the efficacy changes during Paozhi through inferring the changes in saponin components and its possible transformation mechanisms before and after processing S. officinalis. Meanwhile, it also provides new insights into potential bioactive ingredients for the treatment of colon cancer.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 2
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2023-07-10)
    Abstract: Temozolomide (TMZ) therapy offers minimal clinical benefits in patients with glioblastoma multiforme (GBM) with high EGFR activity, underscoring the need for effective combination therapy. Here, we show that tonicity-responsive enhancer binding protein (NFAT5) lysine methylation, is a determinant of TMZ response. Mechanistically, EGFR activation induces phosphorylated EZH2 (Ser21) binding and triggers NFAT5 methylation at K668. Methylation prevents NFAT5 cytoplasm interaction with E3 ligase TRAF6, thus blocks NFAT5 lysosomal degradation and cytosol localization restriction, which was mediated by TRAF6 induced K63-linked ubiquitination, resulting in NFAT5 protein stabilization, nuclear accumulation and activation. Methylated NFAT5 leads to the upregulation of MGMT, a transcriptional target of NFAT5, which is responsible for unfavorable TMZ response. Inhibition of NFAT5 K668 methylation improved TMZ efficacy in orthotopic xenografts and patient-derived xenografts (PDX) models. Notably, NFAT5 K668 methylation levels are elevated in TMZ-refractory specimens and confer poor prognosis. Our findings suggest targeting NFAT5 methylation is a promising therapeutic strategy to improve TMZ response in tumors with EGFR activation.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2553671-0
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  • 3
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-10-13)
    Abstract: Burrowing nematodes ( Radopholus similis ) cause severe harm in many agronomic and horticultural crops and are very difficult to manage. Cathepsin S is one of the most important cysteine proteinases and plays key roles in nematodes and many other parasites. To evaluate the effect of in planta RNAi on the control of this nematode, a specific fragment from the protease gene, cathepsin S ( Rs - cps ), was cloned into the binary vector pFGC5941 in the forward and reverse orientations to construct recombinant plant RNAi vectors. Transgenic Nicotiana benthamiana plants expressing Rs - cps dsRNA were obtained and studied. The transcript abundance of Rs - cps dsRNA appeared to be diverse in the different transgenic lines. Moreover, the bioassay results revealed that Rs-cps transgenic N. benthamiana plants were resistant to R . similis and the transcription level of Rs-cps in R . similis was drastically decreased. In addition, the reproduction and hatching rate of R . similis isolated from the Rs-cps transgenic plants were also significantly reduced. Our results suggest that Rs-cps is essential for the reproduction and pathogenicity of R . similis . This is the first study to employ in planta RNAi approach to target the Rs-cps gene for the control of plant parasitic nematodes.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2615211-3
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  • 4
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2018-02-26)
    Abstract: Staphylococcus aureus can cause different types of diseases from mild skin infections to life-threatening sepsis worldwide. Owing to the emergence and transmission of multidrug-resistant strains, developing an impactful immunotherapy especially vaccine control approach against S . aureus infections is increasingly encouraged and supported. S . aureus manganese transport protein C (MntC), which is a highly-conserved cell surface protein, can elicit protective immunity against S . aureus and Staphylococcus epidermidis . In this study, we evaluated the humoral immune response and CD4 + T cell-mediated immune responses in a mouse peritonitis model. The results showed that MntC-specific antibodies conferred an essential protection for mice to reduce invasion of S . aureus , which was corroborated via the opsonophagocytic killing assay and passive immunization experiment in mice, and moreover MntC-induced Th17 played a remarkable part in preventing S . aureus infection since the MntC-induced protective immunity decreased after neutralization of IL-17 by antibody in vivo and the Th17 adoptive transferred-mice could partly resist S . aureus challenge. In conclusion, we considered that the MntC-specific antibodies and MntC-specific Th17 cells play cooperative roles in the prevention of S . aureus infection.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 2615211-3
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  • 5
    In: Geofluids, Hindawi Limited, Vol. 2021 ( 2021-10-8), p. 1-14
    Abstract: Advancing urbanization in China requires large-scale high-rise construction and underground transportation projects. Consequently, there is an increasing number of deep foundation pits adjacent to water bodies, and accidents occur frequently. This study uses a numerical simulation method to study the stability of the deep foundation pit near water based on the Biot three-dimensional seepage-stress coupling model, with the open-cut section on the south bank of the Jinan Yellow River Tunnel Project as the engineering field test. This indicates the following: (1) the maximum horizontal displacement of the diaphragm wall occurred in the fifth excavation stage, and a horizontal brace effectively controlled the inward horizontal displacement of the foundation pit; (2) considering the effect of seepage in the soft soil foundation, the maximum vertical displacement of the ground surface at each excavation stage occurred adjacent to the underground continuous wall. As the depth of the foundation pit increased, the vertical surface settlement decreases gradually in the direction away from the excavation face; (3) considering the seepage conditions, within each interval of excavation of the foundation pit, the horizontal displacement of the continuous underground wall and ground settlement declined; and (4) the numerical simulation and field monitoring data were in good agreement. Under the conditions of accurate model simplification and parameter selection, numerical simulations can adequately forecast conditions of the actual project.
    Type of Medium: Online Resource
    ISSN: 1468-8123 , 1468-8115
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2045012-6
    SSG: 13
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  • 6
    In: Materials, MDPI AG, Vol. 15, No. 3 ( 2022-02-07), p. 1240-
    Abstract: Slag Portland cement is an environmentally friendly and energy-saving product, which is widely used in cement-reinforced soil. This study used slag Portland cement-reinforced soil as the research object and P.O 42.5 + kaolin (POK) as the reference group. The carbonation depth and strength of P.S.A 42.5 + kaolin (PSK) at different curing times were analyzed using carbonation depth, uniaxial ground pressure strength, scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The test results show the following: (1) The active substances in PSK samples can react with calcium hydroxide produced during cement hydration and can reduce the content of OH−. The PSK samples react with OH− and CO2 in the carbonation environment. Both processes considerably reduce the content of OH−. (2) Due to the decrease in OH− content, the carbonation durability of slag Portland cement-reinforced soil is significantly less than that of ordinary Portland cement. (3) The carbonation of slag Portland cement-reinforced soil improves its strength. (4) The results of SEM + EDS and XRD confirm the carbonation depth and strength of the POK and PSK samples. The results show that PSK has important applications in subgrade or building grouting materials and in cement-soil mixing piles (walls).
    Type of Medium: Online Resource
    ISSN: 1996-1944
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2487261-1
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  • 7
    In: Materials, MDPI AG, Vol. 15, No. 6 ( 2022-03-19), p. 2281-
    Abstract: Wire mesh is a common material for bolt mesh supporting structures, but its application in engineering has revealed many defects. At the same time, with the development of new materials for civil engineering, the new material mesh performance and cost show outstanding advantages over wire mesh. In this paper, the feasibility of replacing wire mesh with steel-plastic geogrid as an alternative material is carefully studied through indoor tests and field applications. The following conclusions were drawn from a comparative analysis with wire mesh, mainly in terms of mechanical properties, engineering characteristics, and construction techniques: (1) in terms of mesh wire strength, wire mesh is slightly better than steel-plastic geogrid, but in the case of similar tensile strength, the amount of steel used per unit length of steel geogrid bars is only 36.75% of that of steel-plastic geogrid, while the tensile strength of the high-strength steel wire attached to the steel-plastic geogrid belt is about 3.3 times that of steel bars; (2) in terms of junction peel strength, both values are similar, with the injection-moulded junction being 1154.56–1224.38 N and the welded junction of 4 mm mesh being 988.35 N; (3) in terms of the strength of the mesh, steel-plastic geogrid is better than wire mesh, and with the same mesh wire strength, the bearing capacity of steel-plastic geogrid is increased by about 63.17% and the contribution of the mesh wire bearing capacity is increased by 83.66%, with the damage mainly being in the form of wire breakage in the ribbon causing ribbon failure, leading to further damage to the mesh; (4) in terms of the engineering application of steel-plastic geogrid compared to wire mesh, the utilization rate of mesh increases by about 24.99%, the construction efficiency increases by about 14.10%, and the economic benefit increases by about 45.31%. In practical application, the steel-plastic geogrid has good adhesion with surrounding rock and strong corrosion resistance. According to the above research analysis, the steel-plastic geogrid is feasible to replace the wire mesh for bolt mesh supporting.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2487261-1
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  • 8
    In: Prestress Technology, Prestress Technology, Vol. 19, No. 01 ( 2015), p. 18-32
    Type of Medium: Online Resource
    ISSN: 2521-9480
    Language: English
    Publisher: Prestress Technology
    Publication Date: 2015
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  • 9
    In: Microbial Pathogenesis, Elsevier BV, Vol. 118 ( 2018-05), p. 39-47
    Type of Medium: Online Resource
    ISSN: 0882-4010
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2018
    detail.hit.zdb_id: 1471158-8
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    SAGE Publications ; 2020
    In:  Natural Product Communications Vol. 15, No. 4 ( 2020-04), p. 1934578X2091055-
    In: Natural Product Communications, SAGE Publications, Vol. 15, No. 4 ( 2020-04), p. 1934578X2091055-
    Abstract: An ultra-performance liquid chromatography-electrospray ionization-mass spectrometry targeted metabolomics strategy was applied to analyze protocatechuate, syringin, eleutheroside E, isofraxidin, hyperoside, kaempferol, and oleanolic acid, the active compounds in 3-year-old, 5-year-old, and 9-year-old Acanthopanax senticosus. Then, targeted metabolomics was conducted with 3 growth year plants to identify 19 phenolic metabolites related to the above-mentioned active compounds, including 9 C 6 C 3 C 6 -type, 6 C 6 C 3 -type, and 4 C 6 C 1 -type. Multivariate statistical analysis was applied to the bioactive metabolite data, and targeted metabolic profiling was used for marker compound classification and characterization. The results showed that 7 active compounds in the roots and stems in the 3 growth year plants differed. The principal component “Q” values showed that the total contents of 7 active compounds in 5-year-old roots and stems were higher than in other growth years. Results of targeted metabolomics profiling of 19 phenolic metabolites showed that the C 6 C 1 -type compounds accumulated in 9-year-old plants, the C 6 C 3 -type in 3-year-old plants, and the C 6 C 3 C 6 -type in 5-year-old plants. The stems had the greatest accumulations of the phenolic metabolites. C 6 C 1 and C 6 C 3 -type metabolites are the most abundant in both roots and stems. In conclusion, the active compounds and pharmacological effects of A. senticosus in different growth years are different. The best harvest age for A. senticosus roots and stems was 5 years. The accumulation of 19 phenolic metabolites in different growth years also showed significant differences.
    Type of Medium: Online Resource
    ISSN: 1934-578X , 1555-9475
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2430442-6
    SSG: 15,3
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