In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 50, No. 8 ( 2002-08), p. 1113-1130
Abstract:
Recent studies suggest important functions for laminin-8 (Ln-8; α4β1γ1) in vascular and blood cell biology, but its distribution in human tissues has remained elusive. We have raised a monoclonal antibody (MAb) FC10, and by enzyme-linked immunoassay (EIA) and Western blotting techniques we show that it recognizes the human Ln α4-chain. Immunoreactivity for the Ln α4-chain was localized in tissues of mesodermal origin, such as basement membranes (BMs) of endothelia, adipocytes, and skeletal, smooth, and cardiac muscle cells. In addition, the Ln α4-chain was found in regions of some epithelial BMs, including epidermis, salivary glands, pancreas, esophageal and gastric glands, intestinal crypts, and some renal medullary tubules. Developmental differences in the distribution of Ln α4-chain were detected in skeletal muscle, walls of vessels, and intestinal crypts. Ln α4- and Ln α2-chains co-localized in BMs of fetal skeletal muscle cells and in some epithelial BMs, e.g., in gastric glands and acini of pancreas. Cultured human pulmonary artery endothelial (HPAE) cells produced Ln α4-chain as M r 180,000 and 200,000 doublet and rapidly deposited it to the growth substratum. In cell-free extracellular matrices of human kidney and lung, Ln α4-chain was found as M r 180,000 protein.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
DOI:
10.1177/002215540205000813
Language:
English
Publisher:
SAGE Publications
Publication Date:
2002
detail.hit.zdb_id:
1421306-0
SSG:
12
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