In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 79, No. 12 ( 2013-06-15), p. 3582-3589
Abstract:
In recent years, glycerol has become an attractive carbon source for microbial processes, as it accumulates massively as a by-product of biodiesel production, also resulting in a decline of its price. A potential use of glycerol in biotechnology is the synthesis of poly(3-hydroxypropionate) [poly(3HP)], a biopolymer with promising properties which is not synthesized by any known wild-type organism. In this study, the genes for 1,3-propanediol dehydrogenase ( dhaT ) and aldehyde dehydrogenase ( aldD ) of Pseudomonas putida KT2442, propionate-coenzyme A (propionate-CoA) transferase ( pct ) of Clostridium propionicum X2, and polyhydroxyalkanoate (PHA) synthase ( phaC1 ) of Ralstonia eutropha H16 were cloned and expressed in the 1,3-propanediol producer Shimwellia blattae . In a two-step cultivation process, recombinant S. blattae cells accumulated up to 9.8% � 0.4% (wt/wt [cell dry weight]) poly(3HP) with glycerol as the sole carbon source. Furthermore, the engineered strain tolerated the application of crude glycerol derived from biodiesel production, yielding a cell density of 4.05 g cell dry weight/liter in a 2-liter fed-batch fermentation process.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.00161-13
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2013
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
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