In:
European Neurology, S. Karger AG, Vol. 84, No. 5 ( 2021), p. 380-388
Abstract:
〈 b 〉 〈 i 〉 Introduction: 〈 /i 〉 〈 /b 〉 Recent studies have shown that inflammatory processes might play a role in epileptogenesis. Their role in ictogenesis is much less clear. The aim of this study was to investigate peri-ictal changes of the innate immune system by analyzing changes of immune cells, as well as pro- and anti-inflammatory cytokines. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Patients with active epilepsy admitted for video-EEG monitoring for presurgical evaluation were included. Blood was sampled every 20 min for 5 h on 3 consecutive days until a seizure occurred. After a seizure, additional samples were drawn immediately, as well as 1 and 24 h later. To analyze the different populations of peripheral blood mononuclear cells, all samples underwent FACS for CD3, CD4, CD8, CD56, CD14, CD16, and CD19. For cytokine analysis, we used a custom bead-based multiplex immunoassay for IFN-γ, IL-1β, IL-1RA, IL-4, IL-6, IL-10, IL-12, IL-17, MCP-1, MIP-1α, and TNFα. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Fourteen patients with focal seizures during the sampling period were included. Natural killer (NK) cells showed a negative correlation ( 〈 i 〉 ρ 〈 /i 〉 = −0.3362, 〈 i 〉 p 〈 /i 〉 = 0.0195) before seizure onset and an immediate increase to 1.95-fold afterward. T helper ( 〈 i 〉 T 〈 /i 〉 〈 sub 〉 H 〈 /sub 〉 ) and B cells decreased by 2 and 8%, respectively, in the immediate postictal interval. Nonclassical and intermediate monocytes decreased not until 1 day after the seizures, and cytotoxic T ( 〈 i 〉 T 〈 /i 〉 〈 sub 〉 C 〈 /sub 〉 ) cells showed a long-lasting postictal increase by 4%. IL-10 and MCP-1 increased significantly after seizures, and IL-12 decreased in the postictal phase. 〈 b 〉 〈 i 〉 Discussion/Conclusion: 〈 /i 〉 〈 /b 〉 Our study argues for a role of the innate immune system in the pre- and postictal phases. NK cells might be involved in preictal changes or be altered as an epiphenomenon in the immediate preictal interval.
Type of Medium:
Online Resource
ISSN:
0014-3022
,
1421-9913
Language:
English
Publisher:
S. Karger AG
Publication Date:
2021
detail.hit.zdb_id:
1482237-4
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