In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_8 ( 2014-08-01), p. 2792-2797
Abstract:
A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40 T , was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5 % wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium cellulovorans as the closest characterized species, showing 93.8 % sequence similarity to the type strain. Cells of strain M2/40 T were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H 2 , CO 2 , formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids ( 〉 10 %) were C 16 : 0 , summed feature 10 (C 18 : 1 ω11 c /ω9 t /ω6 t and/or unknown ECL 17.834) and C 18 : 1 ω11 c dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40 T represents a novel species within the genus Clostridium , for which we propose the name Clostridium bornimense sp. nov. The type strain is M2/40 T ( = DSM 25664 T = CECT 8097 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.059691-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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