In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-13), p. e0261588-
Abstract:
Results from sampling healthcare surfaces for pathogens are difficult to interpret without understanding the factors that influence pathogen detection. We investigated the recovery of four healthcare-associated pathogens from three common surface materials, and how a body fluid simulant (artificial test soil, ATS), deposition method, and contamination levels influence the percent of organisms recovered (%R). Known quantities of carbapenemase-producing KPC+ Klebsiella pneumoniae (KPC), Acinetobacter baumannii , vancomycin-resistant Enterococcus faecalis , and Clostridioides difficile spores (CD) were suspended in Butterfield’s buffer or ATS, deposited on 323cm 2 steel, plastic, and laminate surfaces, allowed to dry 1h, then sampled with a cellulose sponge wipe. Bacteria were eluted, cultured, CFU counted and %R determined relative to the inoculum. The %R varied by organism, from 〈 1% (KPC) to almost 60% (CD) and was more dependent upon the organism’s characteristics and presence of ATS than on surface type. KPC persistence as determined by culture also declined by 〉 1 log 10 within the 60 min drying time. For all organisms, the %R was significantly greater if suspended in ATS than if suspended in Butterfield’s buffer (p 〈 0.05), and for most organisms the %R was not significantly different when sampled from any of the three surfaces. Organisms deposited in multiple droplets were recovered at equal or higher %R than if spread evenly on the surface. This work assists in interpreting data collected while investigating a healthcare infection outbreak or while conducting infection intervention studies.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0261588
DOI:
10.1371/journal.pone.0261588.g001
DOI:
10.1371/journal.pone.0261588.g002
DOI:
10.1371/journal.pone.0261588.g003
DOI:
10.1371/journal.pone.0261588.g004
DOI:
10.1371/journal.pone.0261588.t001
DOI:
10.1371/journal.pone.0261588.t002
DOI:
10.1371/journal.pone.0261588.t003
DOI:
10.1371/journal.pone.0261588.t004
DOI:
10.1371/journal.pone.0261588.t005
DOI:
10.1371/journal.pone.0261588.s001
DOI:
10.1371/journal.pone.0261588.s002
DOI:
10.1371/journal.pone.0261588.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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