In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 4 ( 2022-04-25)
Abstract:
Strain NGK35 T is a motile, Gram-stain-negative, rod-shaped (1.0–2.1 µm long and 0.6–0.8 µm wide), aerobic bacterium that was isolated from plastic-polluted landfill soil. The strain grew at temperatures between 6 and 37 °C (optimum, 28 °C), in 0–10 % NaCl (optimum, 1 %) and at pH 6.0–9.5 (optimum, pH 7.5–8.5). It was positive for cytochrome c oxidase, catalase as well as H 2 S production, and hydrolysed casein and urea. It used a variety of different carbon sources including citrate, lactate and pyruvate. The predominant membrane fatty acids were C 16 : 1 cis9 and C 16 : 0 , followed by C 17 : 0 cyclo and C 18 : 1 cis11. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, followed by diphosphatidyglycerol. The only quinone was ubiquinone Q-8. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK35 T belongs to the genus Paenalcaligenes (family Alcaligenaceae ), appearing most closely related to Paenalcaligenes hominis CCUG 53761A T (96.90 %) and Paenalcaligenes suwonensis ABC02-12 T (96.94 %). The genomic DNA G+C content of strain NGK35 T was 52.1 mol %. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus Paenalcaligenes . Based on phenotypic and molecular characterization, strain NGK35 T can clearly be differentiated from its phylogenetic neighbours establishing a novel species, for which the name Paenalcaligenes niemegkensis sp. nov. is proposed. The type strain is NGK35 T (=DSM 113270 T =NCCB 100854 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005333
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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