In:
Experimental & Molecular Medicine, Springer Science and Business Media LLC, Vol. 55, No. 2 ( 2023-02-03), p. 385-400
Kurzfassung:
In binocular animals that exhibit stereoscopic visual responses, the axons of retinal ganglion cells (RGCs) connect to brain areas bilaterally by forming a commissure called the optic chiasm (OC). Ventral anterior homeobox 1 (Vax1) contributes to the formation of the OC, acting endogenously in optic pathway cells and exogenously in growing RGC axons. Here, we generated Vax1 AA/AA mice expressing the Vax1 AA mutant, which is incapable of intercellular transfer. We found that RGC axons cannot take up Vax1 AA protein from the Vax1 AA/AA mouse optic stalk (OS) and grow slowly to arrive at the hypothalamus at a late stage. The RGC axons of Vax1 AA/AA mice connect exclusively to ipsilateral brain areas after failing to access the midline, resulting in reduced visual acuity and abnormal oculomotor responses. Overall, our study provides physiological evidence for the necessity of intercellular transfer of Vax1 and the importance of the bilateral RGC axon projection in proper visuomotor responses.
Materialart:
Online-Ressource
ISSN:
2092-6413
DOI:
10.1038/s12276-023-00930-4
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2023
ZDB Id:
2084833-X
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