In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_10 ( 2015-10-01), p. 3407-3412
Abstract:
A novel Gram-stain-positive, spore-forming, aerobic, motile and rod-shaped bacterium designated strain PAMC 80007 T was isolated from an active layer soil sample of Council, Alaska. Optimal growth of strain PAMC 80007 T was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain PAMC 80007 T belonged to the genus Domibacillus . This strain was closely related to Domibacillus enclensis (98.3 %), Domibacillus robiginosus (98.3 %) and Domibacillus indicus (97.2 %). Genomic DNA G+C content was 43.5 mol% and genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 80007 T is clearly distinguished from the closely related species of the genus Domibacillus . The major fatty acids ( 〉 5 %) were iso-C 15 : 0 (24.7 %), C 16 : 1 ω11 c (16.8 %), anteiso-C 15 : 0 (16.5 %), C 16 : 0 (15.6 %) and anteiso-C 17 : 0 (8.7 %). The major respiratory isoprenoid quinones were menaquinone-6 (MK-6) and menaquinone-7 (MK-7), and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, phospholipid and two unidentified lipids. meso -Diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan, and the major whole-cell sugar was ribose with a minor quantity of glucose. Results from a polyphasic study suggested that strain PAMC 80007 T represents a novel species of the genus Domibacillus for which the name Domibacillus tundrae sp. nov. is proposed. The type strain is PAMC 80007 T ( = JCM 30371 T = KCTC 33549 T = DSM 29572 T ). An emended description of the genus Domibacillus is also provided.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000429
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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