In:
Applications in Plant Sciences, Wiley, Vol. 6, No. 5 ( 2018-05)
Abstract:
The tetraploid Veronica aragonensis (Plantaginaceae) is a narrow endemic to the Iberian Peninsula. Specific microsatellite markers were developed to investigate genetic structure and diversity. Methods and Results A total of 15 polymorphic markers were characterized on three populations of V. aragonensis , using a microsatellite‐enriched library on an Ion Torrent sequencer and high‐resolution melting ( HRM ) analyses to rapidly discard nonreliable, multicopy, and/or monomorphic loci. Allele number per locus ranged from one to five, and levels of observed heterozygosity per population varied from 0.142 ± 0.301 to 0.281 ± 0.369. Most primers also amplified in the closely related species V. rosea and in three subspecies of V. tenuifolia . Conclusions The species‐specific microsatellite markers developed here represent an essential tool to provide genetic information on the population level for V. aragonensis . The low levels of variation detected highlight the importance of continued efforts to improve conservation of the species.
Type of Medium:
Online Resource
ISSN:
2168-0450
,
2168-0450
DOI:
10.1002/aps3.2018.6.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2699923-7
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