In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2021-8-30), p. e1009902-
Abstract:
The p21-activated kinase (PAK) family regulate a multitude of cellular processes, including actin cytoskeleton remodelling. Numerous bacterial pathogens usurp host signalling pathways that regulate actin reorganisation in order to promote Infection. Salmonella and pathogenic Escherichia coli drive actin-dependent forced uptake and intimate attachment respectively. We demonstrate that the pathogen-driven generation of both these distinct actin structures relies on the recruitment and activation of PAK. We show that the PAK kinase domain is dispensable for this actin remodelling, which instead requires the GTPase-binding CRIB and the central poly-proline rich region. PAK interacts with and inhibits the guanine nucleotide exchange factor β-PIX, preventing it from exerting a negative effect on cytoskeleton reorganisation. This kinase-independent function of PAK may be usurped by other pathogens that modify host cytoskeleton signalling and helps us better understand how PAK functions in normal and diseased eukaryotic cells.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009902
DOI:
10.1371/journal.ppat.1009902.g001
DOI:
10.1371/journal.ppat.1009902.g002
DOI:
10.1371/journal.ppat.1009902.g003
DOI:
10.1371/journal.ppat.1009902.g004
DOI:
10.1371/journal.ppat.1009902.g005
DOI:
10.1371/journal.ppat.1009902.g006
DOI:
10.1371/journal.ppat.1009902.g007
DOI:
10.1371/journal.ppat.1009902.s001
DOI:
10.1371/journal.ppat.1009902.s002
DOI:
10.1371/journal.ppat.1009902.s003
DOI:
10.1371/journal.ppat.1009902.s004
DOI:
10.1371/journal.ppat.1009902.s005
DOI:
10.1371/journal.ppat.1009902.s006
DOI:
10.1371/journal.ppat.1009902.s007
DOI:
10.1371/journal.ppat.1009902.s008
DOI:
10.1371/journal.ppat.1009902.s009
DOI:
10.1371/journal.ppat.1009902.s010
DOI:
10.1371/journal.ppat.1009902.r001
DOI:
10.1371/journal.ppat.1009902.r002
DOI:
10.1371/journal.ppat.1009902.r003
DOI:
10.1371/journal.ppat.1009902.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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