In:
The FEBS Journal, Wiley, Vol. 282, No. 9 ( 2015-05), p. 1774-1785
Abstract:
The nucleosome remodeling and deacetylase (Nu RD ) complex is an evolutionarily conserved chromatin‐associated protein complex. Although the subunit composition of the mammalian complex is fairly well characterized, less is known about the stability and dynamics of these interactions. Furthermore, detailed information regarding protein–protein interaction surfaces within the complex is still largely lacking. Here, we show that the Nu RD complex interacts with a number of substoichiometric zinc finger‐containing proteins. Some of these interactions are salt‐sensitive ( ZNF 512B and SALL 4), whereas others ( ZMYND 8) are not. The stoichiometry of the core subunits is not affected by high salt concentrations, indicating that the core complex is stabilized by hydrophobic interactions. Interestingly, the RBBP 4 and RBBP 7 proteins are sensitive to high nonionic detergent concentrations during affinity purification. In a subunit exchange assay with stable isotope labeling by amino acids in cell culture ( SILAC )‐treated nuclear extracts, RBBP 4 and RBBP 7 were identified as dynamic core subunits of the Nu RD complex, consistent with their proposed role as histone chaperones. Finally, using cross‐linking MS , we have uncovered novel features of Nu RD molecular architecture that complement our affinity purification‐ MS / MS data. Altogether, these findings extend our understanding of MBD 3–Nu RD structure and stability. Structured digital abstract MBD3 physically interacts with ZNF512B , HDAC1 , ZMYND8 , GATAD2B , SALL4 , GATAD2A , ZNF592 , MTA3 , ZNF687 , CDK2AP1 , CHD3 , ZNF532 , HDAC2 , MTA2 , CHD4 , MTA1 , KPNA2 , CHD5 , RBBP4 and RBBP7 by pull down ( View interaction ) CDK2AP1 physically interacts with MBD3 , MTA3 , HDAC2 , GATAD2A , CHD4 , CDK2AP1 , MTA2 , HDAC1 , MTA1 , CHD3 , GATAD2B , MBD2 , RBBP4 and RBBP7 by pull down ( View interaction ) MBD3 physically interacts with MTA2 , MTA3 , RBBP4 , RBBP7 , HDAC2 , HDAC1 , CHD4 , CHD3 and MTA1 by cross-linking study ( View interaction )
Type of Medium:
Online Resource
ISSN:
1742-464X
,
1742-4658
DOI:
10.1111/febs.2015.282.issue-9
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2172518-4
SSG:
12
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