In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 12 ( 2022-12-19), p. e0276591-
Abstract:
Hyperuricaemic nephropathy (HN) is a common clinical complication of hyperuricaemia (HUA) and poses a huge threat to human health. Hence, we aimed to prospectively investigate the dysregulated genes, pathways and networks involved in HN by performing whole transcriptome sequencing using RNA sequencing. Six kidney samples from HN group (n = 3) and a control group (n = 3) were obtained to conduct RNA sequencing. To disclose the relevant signalling pathways, we conducted the analysis of differentially expressed genes (DEGs), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A competitive endogenous RNA (ceRNA) network was established to reveal the interactions between lncRNAs, circRNAs, mRNAs and miRNAs and investigate the potential mechanisms of HN. Ultimately, 2250 mRNAs, 306 lncRNAs, 5 circRNAs, and 70 miRNAs were determined to be significantly differentially expressed in the HN group relative to the control group. We further authenticated 8 differentially expressed (DE)-ncRNAs by quantitative real-time polymerase chain reaction, and these findings were in accordance with the sequencing results. The analysis results evidently showed that these DE-ncRNAs were significantly enriched in pathways related to inflammatory reaction. In conclusion, HUA may generate abnormal gene expression changes and regulate signalling pathways in kidney samples. Potentially related genes and pathways involved in HN were identified.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0276591
DOI:
10.1371/journal.pone.0276591.g001
DOI:
10.1371/journal.pone.0276591.g002
DOI:
10.1371/journal.pone.0276591.g003
DOI:
10.1371/journal.pone.0276591.g004
DOI:
10.1371/journal.pone.0276591.g005
DOI:
10.1371/journal.pone.0276591.g006
DOI:
10.1371/journal.pone.0276591.g007
DOI:
10.1371/journal.pone.0276591.g008
DOI:
10.1371/journal.pone.0276591.g009
DOI:
10.1371/journal.pone.0276591.t001
DOI:
10.1371/journal.pone.0276591.t002
DOI:
10.1371/journal.pone.0276591.s001
DOI:
10.1371/journal.pone.0276591.s002
DOI:
10.1371/journal.pone.0276591.s003
DOI:
10.1371/journal.pone.0276591.s004
DOI:
10.1371/journal.pone.0276591.s005
DOI:
10.1371/journal.pone.0276591.s006
DOI:
10.1371/journal.pone.0276591.s007
DOI:
10.1371/journal.pone.0276591.s008
DOI:
10.1371/journal.pone.0276591.s009
DOI:
10.1371/journal.pone.0276591.s010
DOI:
10.1371/journal.pone.0276591.s011
DOI:
10.1371/journal.pone.0276591.r001
DOI:
10.1371/journal.pone.0276591.r002
DOI:
10.1371/journal.pone.0276591.r003
DOI:
10.1371/journal.pone.0276591.r004
DOI:
10.1371/journal.pone.0276591.r005
DOI:
10.1371/journal.pone.0276591.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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