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  • 1
    In: Clinical Science, Portland Press Ltd., Vol. 103, No. 2 ( 2002-08-01), p. 191-200
    Abstract: Obstetric cholestasis is characterized by raised bile acids, and can be complicated by intrauterine death. We have shown that the bile acid taurocholate causes loss of synchronous beating, bradycardia and cessation of contraction in cultured rat cardiomyocytes [Williamson, Gorelik, Eaton, Lab, de Swiet and Korchev (2001) Clin. Sci. 100, 363–369]. The aim of the present study was to investigate the effect of taurocholate on cardiomyocytes further. We demonstrated a reduced rate of contraction and proportion of beating cells when rat cardiomyocytes were exposed to increasing concentrations of taurocholate (0.1–3.0mM); more marked at higher concentrations (P 〈 0.001). Using scanning ion-conductance microscopy, we also demonstrated reduced amplitude of contraction and calcium transients with taurocholate. Our observations indicate that taurocholate affects calcium release from the sarcoplasmic reticulum and this parallels changes in contractile function. The relationship between the contraction amplitude and calcium transient is not linear, particularly at higher concentrations of taurocholate. We observed different effects in individual cultured neonatal cells; a reversible reduction in rate and amplitude of contraction in some, and irreversible oscillatory (fibrillatory) cessation of beating in others. The effects were more marked with higher concentrations. The contraction amplitude was also reduced in adult cardiomyocytes. The changes were reversible following removal of taurocholate in adult, but not in neonatal, cardiomyocytes exposed to higher concentrations ( 〉 0.3mM) (P 〈 0.001). In conclusion we have demonstrated that the bile acid taurocholate can cause different types of dysrhythmia in individual cardiomyocytes. These results provide further support for the hypothesis that obstetric cholestasis may produce cardiac-related sudden intrauterine death.
    Type of Medium: Online Resource
    ISSN: 0143-5221 , 1470-8736
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 2002
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  • 2
    Online Resource
    Online Resource
    Emerald ; 2024
    In:  Built Environment Project and Asset Management Vol. 14, No. 2 ( 2024-03-14), p. 244-259
    In: Built Environment Project and Asset Management, Emerald, Vol. 14, No. 2 ( 2024-03-14), p. 244-259
    Abstract: A comprehensive literature review was conducted to identify the lean principles and the challenges of building refurbishment. To have an in-depth investigation of the application of lean principles to address the challenges of refurbishment projects, ten expert interviews following a qualitative research approach were utilised in this research. Data were analysed using manual content analysis to derive the framework. Design/methodology/approach The refurbishment of buildings has attracted the attention of the present construction industry. However, uncertain project characteristics, information deficiency, limited space for construction activities and less stakeholder involvement make it complex. Since the lean concept effectively deals with complex and uncertain projects, this study focusses to investigate the application of lean principles to overcome the challenges of refurbishment projects in Sri Lanka by developing a framework. Findings It was found that the five main lean principles of customer value, value stream, value flow, pull and perfection are appropriate for building refurbishment projects in Sri Lanka. Precise identification of clients and end-users, value adding and non-value adding activities, interruptions and stakeholder communication chains, setting scope, examining the possible technologies and taking measures to deliver the exact product to ensure the successful application of lean principles for refurbishment projects. Further, 27 benefits of five lean principles were identified which can be used to address the 13 identified challenges of building refurbishment of projects. Finally, a framework has developed portraying the application of lean principles in building refurbishment. Practical implications The framework developed is beneficial for the building refurbishment project team to address the barriers of refurbishment projects by applying lean principles. Originality/value This framework can be used as a guideline for the implementation of building refurbishment projects by addressing their challenges with lean principles.
    Type of Medium: Online Resource
    ISSN: 2044-124X
    Language: English
    Publisher: Emerald
    Publication Date: 2024
    detail.hit.zdb_id: 2611045-3
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  • 3
    In: Clinical Science, Portland Press Ltd., Vol. 103, No. 2 ( 2002-8-1), p. 191-
    Type of Medium: Online Resource
    ISSN: 0143-5221
    Language: Unknown
    Publisher: Portland Press Ltd.
    Publication Date: 2002
    Location Call Number Limitation Availability
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