In:
The Journal of Infection in Developing Countries, Journal of Infection in Developing Countries, Vol. 17, No. 01 ( 2023-01-31), p. 93-101
Abstract:
Introduction: Diarrhoeal illness is the second cause of morbidity/mortality among children from less-developed regions worldwide. Nonetheless, there is scarce information regarding their gut microbiome.
Aim: Microbiome characterization, with an emphasis on the virome, of children’s stools with diarrhoea, by a commercial microbiome array. Methodology: Nucleic acids extraction, optimised for viral identification, of stool samples from 20 Mexican children with diarrhoea (10 children 〈 2 and 10 ≥ 2-years-old), collected 16 years ago and kept at -70 °C, were analysed for the presence of viruses, bacteria, archaea, protozoa, and fungi species sequences.
Results: Only viral and bacterial species sequences were identified among children’s stools. Most stool samples harboured species belonging to the bacteriophages (95%), anellovirus (60%), diarrhoeagenic viruses (40%), and non-human pathogens viruses (45% avian virus and 40% plant viruses) groups. Among the children's stools, virome inter-individual species composition was observed, even in presence of illness. The 〈 2-years-old children group has significantly higher viral richness (p = 0.01), conferred mainly by bacteriophages and diarrheagenic-viruses (p = 0.01) species, in comparison with the ≥ 2-years-old group.
Conclusions: The virome of stools of children with diarrhoea revealed inter-individual viral species composition. Similarly, to the few virome studies in healthy young children, the bacteriophages group was the most abundant. A significantly higher viral richness, conferred by bacteriophages and diarrheagenic-viral species, was observed among 〈 2-years-old children in comparison with older children. Stools preserved at -70 °C for long term can successfully be used for microbiome studies.
Type of Medium:
Online Resource
ISSN:
1972-2680
Language:
Unknown
Publisher:
Journal of Infection in Developing Countries
Publication Date:
2023
detail.hit.zdb_id:
2394024-4
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