In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-29), p. e0248865-
Abstract:
Dinoroseobacter shibae living in the photic zone of marine ecosystems is frequently exposed to oxygen that forms highly reactive species. Here, we analysed the adaptation of D . shibae to different kinds of oxidative stress using a GeLC-MS/MS approach. D . shibae was grown in artificial seawater medium in the dark with succinate as sole carbon source and exposed to hydrogen peroxide, paraquat or diamide. We quantified 2580 D . shibae proteins. 75 proteins changed significantly in response to peroxide stress, while 220 and 207 proteins were differently regulated by superoxide stress and thiol stress. As expected, proteins like thioredoxin and peroxiredoxin were among these proteins. In addition, proteins involved in bacteriochlophyll biosynthesis were repressed under disulfide and superoxide stress but not under peroxide stress. In contrast, proteins associated with iron transport accumulated in response to peroxide and superoxide stress. Interestingly, the iron-responsive regulator RirA in D . shibae was downregulated by all stressors. A rirA deletion mutant showed an improved adaptation to peroxide stress suggesting that RirA dependent proteins are associated with oxidative stress resistance. Altogether, 139 proteins were upregulated in the mutant strain. Among them are proteins associated with protection and repair of DNA and proteins (e. g. ClpB, Hsp20, RecA, and a thioredoxin like protein). Strikingly, most of the proteins involved in iron metabolism such as iron binding proteins and transporters were not part of the upregulated proteins. In fact, rirA deficient cells were lacking a peroxide dependent induction of these proteins that may also contribute to a higher cell viability under these conditions.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248865
DOI:
10.1371/journal.pone.0248865.g001
DOI:
10.1371/journal.pone.0248865.g002
DOI:
10.1371/journal.pone.0248865.g003
DOI:
10.1371/journal.pone.0248865.g004
DOI:
10.1371/journal.pone.0248865.g005
DOI:
10.1371/journal.pone.0248865.g006
DOI:
10.1371/journal.pone.0248865.g007
DOI:
10.1371/journal.pone.0248865.t001
DOI:
10.1371/journal.pone.0248865.s001
DOI:
10.1371/journal.pone.0248865.s002
DOI:
10.1371/journal.pone.0248865.s003
DOI:
10.1371/journal.pone.0248865.s004
DOI:
10.1371/journal.pone.0248865.s005
DOI:
10.1371/journal.pone.0248865.s006
DOI:
10.1371/journal.pone.0248865.r001
DOI:
10.1371/journal.pone.0248865.r002
DOI:
10.1371/journal.pone.0248865.r003
DOI:
10.1371/journal.pone.0248865.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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