In:
PLOS Biology, Public Library of Science (PLoS), Vol. 18, No. 12 ( 2020-12-11), p. e3001032-
Abstract:
Despite recent progress in recognizing the importance of mesenchymal cells for the homeostasis of the intestinal system, the current picture of how these cells communicate with the associated epithelial layer remains unclear. To describe the relevant cell populations in an unbiased manner, we carried out a single-cell transcriptome analysis of the adult murine colon, producing a high-quality atlas of matched colonic epithelium and mesenchyme. We identify two crypt-associated colonic fibroblast populations that are demarcated by different strengths of platelet-derived growth factor receptor A (Pdgfra) expression. Crypt-bottom fibroblasts (CBFs), close to the intestinal stem cells, express low levels of Pdgfra and secrete canonical Wnt ligands, Wnt potentiators, and bone morphogenetic protein (Bmp) inhibitors. Crypt-top fibroblasts (CTFs) exhibit high Pdgfra levels and secrete noncanonical Wnts and Bmp ligands. While the Pdgfra low cells maintain intestinal stem cell proliferation, the Pdgfra high cells induce differentiation of the epithelial cells. Our findings enhance our understanding of the crosstalk between various colonic epithelial cells and their associated mesenchymal signaling hubs along the crypt axis—placing differential Pdgfra expression levels in the spotlight of intestinal fibroblast identity.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001032
DOI:
10.1371/journal.pbio.3001032.g001
DOI:
10.1371/journal.pbio.3001032.g002
DOI:
10.1371/journal.pbio.3001032.g003
DOI:
10.1371/journal.pbio.3001032.g004
DOI:
10.1371/journal.pbio.3001032.s001
DOI:
10.1371/journal.pbio.3001032.s002
DOI:
10.1371/journal.pbio.3001032.s003
DOI:
10.1371/journal.pbio.3001032.s004
DOI:
10.1371/journal.pbio.3001032.s005
DOI:
10.1371/journal.pbio.3001032.s006
DOI:
10.1371/journal.pbio.3001032.s007
DOI:
10.1371/journal.pbio.3001032.s008
DOI:
10.1371/journal.pbio.3001032.s009
DOI:
10.1371/journal.pbio.3001032.s010
DOI:
10.1371/journal.pbio.3001032.s011
DOI:
10.1371/journal.pbio.3001032.s012
DOI:
10.1371/journal.pbio.3001032.r001
DOI:
10.1371/journal.pbio.3001032.r002
DOI:
10.1371/journal.pbio.3001032.r003
DOI:
10.1371/journal.pbio.3001032.r004
DOI:
10.1371/journal.pbio.3001032.r005
DOI:
10.1371/journal.pbio.3001032.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2126773-X
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