In:
Clinical Chemistry, Oxford University Press (OUP), Vol. 17, No. 10 ( 1971-10-01), p. 1028-1032
Abstract:
An inexpensive, rapid, convenient screening tube test for the detection of hemoglobin S and non-S sickling hemoglobins has been developed, which has a molecular basis. The reagents consist of potassium phosphate, sodium dithionite, and saponin. When sickling red cells are introduced into such a solution, the red cells lyse immediately, the hemoglobin deoxygenates, the beta globin chains of each hemoglobin tetramer are displaced laterally, complementarity of steric fit between interacting hemoglobin tetramers is achieved in accordance with the recently modified Murayama hypothesis for the molecular mechanism of sickling, a nematic liquid crystal system is formed, and in the presence of hemoglobin S or non-S sickling hemoglobins, the system becomes turbid. On addition of urea, those nematic liquid crystal systems dependent upon hydrophobic bonds are dispersed.
Type of Medium:
Online Resource
ISSN:
0009-9147
,
1530-8561
DOI:
10.1093/clinchem/17.10.1028
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1971
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