In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2023-2-9), p. e0276829-
Abstract:
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) target multiple epitopes on different domains of the spike protein, and other SARS-CoV-2 proteins. We developed a SARS-CoV-2 multi-antigen protein microarray with the nucleocapsid, spike and its domains (S1, S2), and variants with single (D614G, E484K, N501Y) or double substitutions (N501Y/Deletion69/70), allowing a more detailed high-throughput analysis of the antibody repertoire following infection. The assay was demonstrated to be reliable and comparable to ELISA. We analyzed antibodies from 18 COVID-19 patients and 12 recovered convalescent donors. The S IgG level was higher than N IgG in most of the COVID-19 patients, and the receptor-binding domain of S1 showed high reactivity, but no antibodies were detected against the heptad repeat domain 2 of S2. Furthermore, antibodies were detected against S variants with single and double substitutions in COVID-19 patients who were infected with SARS-CoV-2 early in the pandemic. Here we demonstrated that the SARS-CoV-2 multi-antigen protein microarray is a powerful tool for detailed characterization of antibody responses, with potential utility in understanding the disease progress and assessing current vaccines and therapies against evolving SARS-CoV-2.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0276829
DOI:
10.1371/journal.pone.0276829.g001
DOI:
10.1371/journal.pone.0276829.g002
DOI:
10.1371/journal.pone.0276829.g003
DOI:
10.1371/journal.pone.0276829.g004
DOI:
10.1371/journal.pone.0276829.t001
DOI:
10.1371/journal.pone.0276829.s001
DOI:
10.1371/journal.pone.0276829.s002
DOI:
10.1371/journal.pone.0276829.s003
DOI:
10.1371/journal.pone.0276829.s004
DOI:
10.1371/journal.pone.0276829.s005
DOI:
10.1371/journal.pone.0276829.s006
DOI:
10.1371/journal.pone.0276829.r001
DOI:
10.1371/journal.pone.0276829.r002
DOI:
10.1371/journal.pone.0276829.r003
DOI:
10.1371/journal.pone.0276829.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
Permalink