In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 19, No. 1 ( 2023-1-6), p. e1010592-
Abstract:
Meiotic recombination is a driving force for genome evolution, deeply characterized in a few model species, notably in the budding yeast Saccharomyces cerevisiae . Interestingly, Zip2, Zip3, Zip4, Spo16, Msh4, and Msh5, members of the so-called ZMM pathway that implements the interfering meiotic crossover pathway in S . cerevisiae , have been lost in Lachancea yeast species after the divergence of Lachancea kluyveri from the rest of the clade. In this context, after investigating meiosis in L . kluyveri , we determined the meiotic recombination landscape of Lachancea waltii . Attempts to generate diploid strains with fully hybrid genomes invariably resulted in strains with frequent whole-chromosome aneuploidy and multiple extended regions of loss of heterozygosity (LOH), which mechanistic origin is so far unclear. Despite the lack of multiple ZMM pro-crossover factors in L . waltii , numbers of crossovers and noncrossovers per meiosis were higher than in L . kluyveri but lower than in S . cerevisiae , for comparable genome sizes. Similar to L . kluyveri but opposite to S . cerevisiae , L . waltii exhibits an elevated frequency of zero-crossover bivalents. Lengths of gene conversion tracts for both crossovers and non-crossovers in L . waltii were comparable to those observed in S . cerevisiae and shorter than in L . kluyveri despite the lack of Mlh2, a factor limiting conversion tract size in S . cerevisiae . L . waltii recombination hotspots were not shared with either S . cerevisiae or L . kluyveri , showing that meiotic recombination hotspots can evolve at a rather limited evolutionary scale within budding yeasts. Finally, L . waltii crossover interference was reduced relative to S . cerevisiae , with interference being detected only in the 25 kb distance range. Detection of positive inference only at short distance scales in the absence of multiple ZMM factors required for interference-sensitive crossovers in other systems likely reflects interference between early recombination precursors such as DSBs.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010592
DOI:
10.1371/journal.pgen.1010592.g001
DOI:
10.1371/journal.pgen.1010592.g002
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10.1371/journal.pgen.1010592.g003
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10.1371/journal.pgen.1010592.g004
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10.1371/journal.pgen.1010592.g005
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10.1371/journal.pgen.1010592.s001
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10.1371/journal.pgen.1010592.s002
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10.1371/journal.pgen.1010592.s003
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10.1371/journal.pgen.1010592.s004
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10.1371/journal.pgen.1010592.s005
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10.1371/journal.pgen.1010592.s006
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10.1371/journal.pgen.1010592.s007
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10.1371/journal.pgen.1010592.s008
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10.1371/journal.pgen.1010592.s009
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10.1371/journal.pgen.1010592.s010
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10.1371/journal.pgen.1010592.s011
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10.1371/journal.pgen.1010592.s012
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10.1371/journal.pgen.1010592.s013
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10.1371/journal.pgen.1010592.s014
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10.1371/journal.pgen.1010592.s015
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10.1371/journal.pgen.1010592.s016
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10.1371/journal.pgen.1010592.s017
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10.1371/journal.pgen.1010592.s018
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10.1371/journal.pgen.1010592.s019
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10.1371/journal.pgen.1010592.s020
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10.1371/journal.pgen.1010592.s021
DOI:
10.1371/journal.pgen.1010592.s022
DOI:
10.1371/journal.pgen.1010592.r001
DOI:
10.1371/journal.pgen.1010592.r002
DOI:
10.1371/journal.pgen.1010592.r003
DOI:
10.1371/journal.pgen.1010592.r004
DOI:
10.1371/journal.pgen.1010592.r005
DOI:
10.1371/journal.pgen.1010592.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2186725-2
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