In:
Frontiers in Immunology, Frontiers Media SA, Vol. 13 ( 2022-11-29)
Abstract:
With growing molecular evidence for correlations between spatial arrangement of blood vasculature and fundamental immunological functions, carried out in distinct compartments of the subdivided lymph node, there is an urgent need for three-dimensional models that can link these aspects. We reconstructed such models at a 1.84 µm resolution by the means of X-ray phase-contrast imaging with a 2D Talbot array in a short time without any staining. In addition reconstructions are verified in immunohistochemistry staining as well as in ultrastructural analyses. While conventional illustrations of mammalian lymph nodes depict the hilus as a definite point of blood and lymphatic vessel entry and exit, our method revealed that multiple branches enter and emerge from an area that extends up to one third of the organ’s surface. This could be a prerequisite for the drastic and location-dependent remodeling of vascularization, which is necessary for lymph node expansion during inflammation. Contrary to corrosion cast studies we identified B-cell follicles exhibiting a two times denser capillary network than the deep cortical units of the T-cell zone. In addition to our observation of high endothelial venules spatially surrounding the follicles, this suggests a direct connection between morphology and B-cell homing. Our findings will deepen the understanding of functional lymph node composition and lymphocyte migration on a fundamental basis.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2022.947961
DOI:
10.3389/fimmu.2022.947961.s001
DOI:
10.3389/fimmu.2022.947961.s002
DOI:
10.3389/fimmu.2022.947961.s003
DOI:
10.3389/fimmu.2022.947961.s004
DOI:
10.3389/fimmu.2022.947961.s005
DOI:
10.3389/fimmu.2022.947961.s006
DOI:
10.3389/fimmu.2022.947961.s007
DOI:
10.3389/fimmu.2022.947961.s008
DOI:
10.3389/fimmu.2022.947961.s009
DOI:
10.3389/fimmu.2022.947961.s010
DOI:
10.3389/fimmu.2022.947961.s011
DOI:
10.3389/fimmu.2022.947961.s012
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2606827-8
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