ISSN:
1399-0047
Quelle:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Thema:
Chemie und Pharmazie
,
Geologie und Paläontologie
,
Physik
Notizen:
Ghilanten, isolated from the leech Haementeria ghilianii, is a potent two-domain anticoagulant protein homologous to the factor Xa inhibitor antistasin. A synthetic gene encoding the amino-terminal domain of ghilanten (ghilanten-D1) was constructed, expressed in the methylotrophic yeast Pichia pastoris and purified by heparin-Sepharose chromatography. Recombinant ghilanten-D1 inhibits bovine trypsin and human factor Xa with equilibrium inhibition constants (Ki) of 126 and 1.2 nM, respectively. Ghilanten-D1 has been crystallized in complex with porcine β-trypsin; three different-looking but isomorphous crystal forms were obtained, each belonging to the orthorhombic space group P212121. These crystals diffracted to beyond 3.6 Å resolution using a rotating-anode X-ray source. A data set complete to 3.7 Å resolution was collected.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1107/S0907444901007272
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