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  • 1
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2008
    In:  Bosnian Journal of Basic Medical Sciences Vol. 8, No. 4 ( 2008-11-20), p. 331-336
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 8, No. 4 ( 2008-11-20), p. 331-336
    Abstract: The present study was carried out to evaluate the renoprotective antioxidant effect of Spirulina platensis on gentamicin-induced acute tubular necrosis in rats. Albino-Wistar rats, (9male and 9 female), weighing approximately 250 g, were used for this study. Rats were randomly assigned to three equal groups. Control group received 0,9 % sodium chloride intraperitoneally for 7 days at the same volume as gentamicin group. Gentamicin group was treated intraperitoneally with gentamicin, 80mg/kg daily for 7 days. Gentamicin+spirulina group received Spirulina platensis 1000 mg/kg orally 2 days before and 7 days concurrently with gentamicin (80mg/kg i.p.). Nephrotoxicity was assessed by measuring plasma nitrite concentration, stabile metabolic product of nitric oxide with oxygen. Plasma nitrite concentration was determined by colorimetric method using Griess reaction. For histological analysis kidney specimens were stained with hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) stain. Plasma nitrite concentration and the level of kidney damage were significantly higher in gentamicin group in comparison both to the control and gentamicin+spirulina group. Spirulina platensis significantly lowered the plasma nitrite level and attenuated histomorphological changes related to renal injury caused by gentamicin. Thus, the results from present study suggest that Spirulina platensis has renoprotective potential in gentamicin-induced acute tubular necrosis possibly due to its antioxidant properties.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2008
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  • 2
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2007
    In:  Bosnian Journal of Basic Medical Sciences Vol. 7, No. 3 ( 2007-08-20), p. 212-217
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 7, No. 3 ( 2007-08-20), p. 212-217
    Abstract: In our investigation, we used short-time model of myocardial infarction of rats induced by high dose of isoproterenol (ISP). We investigated cardiac troponin T blood level (cTnT) and histological characteristics of rat myocardium. ISP, single, intraperitoneal dose 250 mg/kg was given to male, adult, Wistar rats (n=12). Rats were distributed depending on their body weight in subgroups: ISP I (BW 260-280g) and ISP II (BW 250-400g). Control group (n=9) was treated with intraperitoneal dose of 0,95% NaCl. Cardiac TnT was measured by electrochemiluminiscence (ECLA) sandwich immunoassay in rat serum 4 hours after ISP application. Rats’ hearts were dissected and examined by qualitative histological method (HE). Statistical significance was set at 0,05. There was significant difference in cTnT of ISP II (p=0,0001) vs. control and ISP I (p 〈 0,05) vs. control. Significant difference was beetween ISP I and ISP II subgroups (p 〈 0.001). The accent of histological changes of myocardium was on nuclei of cell. Cells showed acydophilic changes and nuclei disappearance as signs of coagulative necrosis development. Extensivity of histological changes were different beetween ISP I and ISP II subgroup. Used dose of ISP induced development of myocardial necrosis in rats. Suben-docardial portion of myocardium was more vulnerability than subepicardial portion. Rats of ISP II had more extensive histological changes than these in ISP I. Administered doses of ISP enabled cTnT utilization as a marker of myocardial necrosis.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2007
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  • 3
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2014
    In:  Biomolecules and Biomedicine Vol. 14, No. 1 ( 2014-05-20), p. 30-34
    In: Biomolecules and Biomedicine, Association of Basic Medical Sciences of FBIH, Vol. 14, No. 1 ( 2014-05-20), p. 30-34
    Abstract: The aim of this research was to assess the reactive changes of rat proximal tubules caused by gentamicin and the effect of relatively low doses of melatonin. 48 adult male Wistar rats were distributed into six groups of equal size which all received one of the following daily intraperitoneal injections: vehicle (5% ethanol in Ringer solution) during 11 days (C); gentamicin (80 mg/kg) during 8 days (G), two groups which concomitantly received gentamicin (80 mg/kg) during 8 days and melatonin in two different test doses (5 or 20 mg/kg) during 11 days (GM1, GM2) and two groups treated only with melatonin in two different doses (5 or 20 mg/kg) during 11 days (M1, M2). Histological analysis included qualitative and semi-quantitative light microscopy analysis of proximal tubules. Exogenous melatonin had no significant effect on the microstructure, independently of dosis. The changes of proximal tubules microstructure induced by gentamicin were expressed in the form of granulovacuolar degeneration, necrosis and desquamation. The grade of proximal tubular changes was smaller in animals who besides gentamicin received melatonin. Melatonin has a dose dependent protective effect on the structural alterations of proximal tubules of the kidney induced by gentamicin.
    Type of Medium: Online Resource
    ISSN: 2831-090X , 2831-0896
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2014
    detail.hit.zdb_id: 3149181-9
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  • 4
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2006
    In:  Bosnian Journal of Basic Medical Sciences Vol. 6, No. 4 ( 2006-11-20), p. 18-21
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 6, No. 4 ( 2006-11-20), p. 18-21
    Abstract: Pineal gland has and antistressogenic role. Its main hormone, melatonin, has radio protective effect on endocrine and other dynamic tissues. In our previous study, we have shown that pinealectomy changes the behavior of suprarenal gland in totally irradiated rats. The aim of this study is to evaluate the effect of exogenous melatonin on suprarenal gland of rats with or without pineal gland. Four months after pinealectomy (experimental group) or shampinealectomy (control group), adult Wistar male rats were daily treated with 0,2 mg of melatoninintraperitoneally, during two weeks. Thereafter, all animals were totally irradiated with 8 Gy of Gamma rays produced from Cobalt 60. Animals who survived were sacrificed on the 17(th) post irradiation day. Qualitative and quantitative characteristics of the suprarenal gland were studied using histological methods. The results show that exogenous melatonin had protective role on suprarenal gland in totally irradiated rats and that those effects were more pronounced in the presence of pineal gland.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2006
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  • 5
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2007
    In:  Bosnian Journal of Basic Medical Sciences Vol. 7, No. 4 ( 2007-11-20), p. 294-300
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 7, No. 4 ( 2007-11-20), p. 294-300
    Abstract: Congenital anomalies of the central nervous system (CNS) are common. The prevalence of these anomalies shows considerable geographical variation and female predominance. The aim of this work was to obtain the frequency of different CNS congenital anomalies types and their sex distribution among cases hospitalized in a Department of Neurosurgery, University of Sarajevo Clinics Center, Bosnia and Herzegovina, during the period January 2001 to December 2004. Retrospective study was carried out on the basis of the clinical records. Standard methods of descriptive statistics were performed for the data analysis. A total of 103 cases were surgically treated in the period from 2001 through 2004. Out of that number 56 (54,4%) were female patients, while 47 (46,6%) were male patients. Seven different CNS birth defect types were found in this investigation. These were: spina bifida (42 cases or 40,78%), congenital hydrocephalus (35 cases or 33,98%), arachnoid cyst (15 cases or 14,56%), Dandy-Walker syndrome (5 cases or 4,85%), dermoid cyst (4 cases or 3,88%), one of Arnold-Chiari syndrome (0,98%) and one of encefalocele (0,98%). According to this investigation, CNS congenital birth defects were slightly higher in females (54,4%). The most frequent types were spina bifida (40,78%) both in females (22,33%) and in males (18,45%), hydrocephalus (33,98%) and arachnoid cyst (14,56%). The anomalies of the other organ systems, associated with CNS anomalies obtained in this investigation, were pes equinovarus, cheiloshisis, cardiomegalia and palatoshisis. They were found in six cases (5,82%), equal in both sexes.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2007
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  • 6
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2008
    In:  Bosnian Journal of Basic Medical Sciences Vol. 8, No. 2 ( 2008-05-20), p. 126-130
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 8, No. 2 ( 2008-05-20), p. 126-130
    Abstract: Congenital anomalies of the urinary system are relatively common anomalies. In Bosnia and Herzegovina there is no existent unique evidence of congenital anomalies and registries. The aim of this study was to obtain the frequency of different urinary tract anomalies types and their sex distribution among cases hospitalized in the Department of Pediatric Surgery of the University of Sarajevo Clinics Centre, Bosnia and Herzegovina, during the period from January 2002 to December 2006. Retrospective study was carried out on the basis of clinical records. Standard methods of descriptive statistics were performed for the data analysis. Among 289 patients that were surgically treated 62,37% of the patients were male patients, while 37,63% were female patients. Twenty nine different urinary system anomalies types were found in this study. These were: vesicoureteral reflux (99 cases or 30,75%), hypospadias (62 cases or 19,26%), pelviureteric junction obstruction (42 cases or 13,04%), megaureter (35 cases or 10,87%), duplex pelvis and ureter (16 cases or 4,97%), bladder diverticulum (8 cases or 2,48%), ureterocoele (7 cases or 2,17%), stenosis of the external urethral opening (6 cases or 1,86%), ectopic kidney, duplex kidney and pelvis (each 5 cases or 1,55%), polycystic kidneys and urethral stricture (each 4 cases or 1,24%), multicystic kidney (3 cases or 0,93%), kidney agenesis, ureter agenesis, urethral diverticulum, ectopic ureter, horseshoe kidney and fetal kidney (each 2 cases or 0,62%), renal aplasia, urethral atresia, renal cyst, urachal cyst, epispadias, bladder exstrophy, renal hypoplasia, renal malrotation and Prune-Belly syndrome (each 1 case or 0,31%). According to this study, urinary tract anomalies were more common in male than in female patients (62,37%), Generally, the most frequent anomaly type was vesicoureteral reflux in total number of 99 cases, and in females (66 cases), but hypospadias was the most common anomaly in males (62 cases). The anomalies of other systems associated with urinary system anomalies were found in ten cases. These were: cryptorchidism, congenital inguinal hernia, open inner inguinal ring, uterus bicornis unicollis and one case of multiple anomalies.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2008
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  • 7
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2003
    In:  Bosnian Journal of Basic Medical Sciences Vol. 3, No. 4 ( 2003-11-20), p. 61-66
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 3, No. 4 ( 2003-11-20), p. 61-66
    Abstract: AbstractBACKGROUND AND PURPOSE:Clinical research of drugs is a researching step subsequent to the preclinical studies in experimental animals. The aim of our research was to evaluate animal model of wound healing process after the burninducement and effects of the ointment containing natural plants on the process of burn healing.MATERIAL AND METHODS:Burn wounds were experimentally induced in two species of experimental animals which were treated with topically applied herbal preparation with concomitant monitoring of the healing process. Experimental groups (1) of 15 animals each (mice and rats), while control group (2) of 10 animals each (mice and rats) that were not being treated with herbal ointment. After the hair removal, burn was induced on the back of animals by heated brass seal. Different clinical symptoms including oedema of surrounding tissue, redness, exudation, size of the burn surface, histological and microbiological findings were monitored on the days 1, 3, 7, 14 and 21. A statistically significant difference was observed throughout descriptive statistics and paired Student's t-test.CONCLUSION:Physiological healing processes of the acute burn wound following the topical application of herbal preparation can be monitored on the utilized animal model. A three-week treatment resulted in the 90% of completed epithelization in both animal species, indicating the effectiveness of topically applied herbal preparation.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2003
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  • 8
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2011
    In:  Bosnian Journal of Basic Medical Sciences Vol. 11, No. 4 ( 2011-11-20), p. 240-
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 11, No. 4 ( 2011-11-20), p. 240-
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2011
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  • 9
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2004
    In:  Bosnian Journal of Basic Medical Sciences Vol. 4, No. 1 ( 2004-02-20), p. 69-72
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 4, No. 1 ( 2004-02-20), p. 69-72
    Abstract: BACKGROUND AND PURPOSE:Developmental process that leads to final forebrain shaping is a result of complex histogenetic and morphogenetic events. Comprehensions about brain development are based on observations carried out on onthogenetic successive stages. Microscopic analysis of brain together with analysis of serial sections gives information about shape the of some forebrain parts and basic relations between them. The aim of this study was to analyse morphogenesis in the earliest stages of rat's forebrain development.MATERIAL AND METHODS:Rat brains used in this study were obtained from Fisher inbred rats with accurately timed pregnancies. The investigation was carried out on serial frontal sections of rat embryonic heads from the 12th (E12) to the 16th (E16) day of gestation. Gestation was considered to have begun early in the morning when sperm was found in the vaginal smear. Histological paraffin and plastic sections were systematically inspected with regard to morphogenetic changes of the forebrain parts telencephalon and diencephalon.RESULTS:E12: neural tube is completely closed in its cranial part. Rostral part of forebrain shows telencephalons vesicles origins as slightly paired enlargements of neuroepithelial wall. Between telencephalic vesicles origin and in direction to caudal there is an origin of diencephalon. E13: rostral part of forebrain shows well expressed and divided areas of telencephalons vesicles as basal, basolateral, dorsal and medial telencephalon. Central area between paired vesicles is a telencephalon impar. In diencephalon optic vesicles appeared. Epithalamus, thalamus and hypothalamus origins are slight enlargements of its neuroepithelial wall. E14: telencephalic vesicles spread above telencephalon impar into rostral direction and above diencephalon in rostrodorsal direction. Their basolateral parts of are very thickened and become folded. Sulcus telodiencephalicus appears. E15: the main event is the appearance of the origins of plexus choroideus in the area of telencephalon impar as fingerlike processes. E16: all forebrain parts, especially telencephalic vesicles-origin of brain hemispheres and processes of plexus choroideus, are progressively growing and shaping.CONCLUSIONS:Our morphologic analysis describes significant morphogenetic changes in the forebrain shape. The forebrain changes from a relatively simple tubular structure with thin walls surrounding a large ventricular system to a thick-walled brain with a highly convoluted but reduced ventricular system.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2004
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  • 10
    Online Resource
    Online Resource
    Association of Basic Medical Sciences of FBIH ; 2005
    In:  Bosnian Journal of Basic Medical Sciences Vol. 5, No. 4 ( 2005-11-20), p. 50-57
    In: Bosnian Journal of Basic Medical Sciences, Association of Basic Medical Sciences of FBIH, Vol. 5, No. 4 ( 2005-11-20), p. 50-57
    Abstract: Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p 〈 0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.
    Type of Medium: Online Resource
    ISSN: 1840-4812 , 1512-8601
    Language: Unknown
    Publisher: Association of Basic Medical Sciences of FBIH
    Publication Date: 2005
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