Publication Date:
2022-05-26
Description:
Author Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Neuroscience Research 75 (2013): 76-81, doi:10.1016/j.neures.2012.11.006.
Description:
Recent advances in our understanding of brain function have come from using light to either
control or image neuronal activity. Here we describe an approach that combines both
techniques: a micromirror array is used to photostimulate populations of presynaptic
neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows
optical detection of resulting postsynaptic activity. Such technology allowed us to control the
activity of cerebellar interneurons while simultaneously recording inhibitory responses in
multiple Purkinje neurons, their postsynaptic targets. This approach should substantially
accelerate our understanding of information processing by populations of neurons within
brain circuits.
Description:
This work was
supported by a Grass Foundation fellowship, National Institutes of Health (NIH grant: R01
EB001963), Duke‐NUS Signature Research Program (SRP) block grant, CRP grant from
the National Research Foundation (Singapore) and by the World Class Institute (WCI)
Program of the National Research Foundation of Korea (NRF) funded by the Ministry of
Education, Science and Technology of Korea (MEST) (NRF Grant Number: WCI 2009-003).
Keywords:
Optogenetics
;
Channelrhodopsin
;
Digital micromirror device
;
Voltage-sensitive dye imaging
;
Inhibitory circuitry
;
Cerebellum
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
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