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  • 1
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    In:  http://aquaticcommons.org/id/eprint/21944 | 18721 | 2018-01-18 08:55:31 | 21944 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-05
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from Hardy Weinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Keywords: Biology ; Fisheries ; Population ; genetic ; Microsatellite ; Clupeidae ; Common kilka ; Clupeonella cultriventris ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 139-148
    Location Call Number Limitation Availability
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  • 2
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    In:  http://aquaticcommons.org/id/eprint/25946 | 18721 | 2018-11-22 07:23:01 | 25946 | Khorramshahr University of Marine Science and Technology
    Publication Date: 2021-07-16
    Description: Common kilka, Clupeonella cultriventris is an economic fish in Caspian sea and we investigate population genetic structure of common kilka in the South Caspian Sea costline (Mazandaran province) using microsatellite markers. Totally, 60 individuals of adult common kilka from two seasons were sampled. Eight sets of microsatellite primers were developed from American shad and Pacific herring tested on genomic DNA of common kilka. At this point only the five successfully used primer sets and were used to analyze the genetic variation in adultsof the common kilka population. Analyses revealed that average of alleles per locus was 11.7 (Na range 6 to 17 alleles per locus). Both of sampled seasons contained private alleles. Average observed and expected heterozygosity were 0.543 and 0.866 respectively. Deviations from Hardy-Weinberg equilibrium were in most cases (P〈0.01). Basis on AMOVA for both FST values among pairs them indicated a significant difference between the two seasons (P≤0.01). These results support the existence of different genetic populations along the South Caspian Sea costline (Mazandaran province) in different seasons.
    Keywords: Biology ; Fisheries ; Iran ; Caspian Sea ; Mazandaran province ; Population ; Genetic ; Structure ; Common kilka ; Clupeonella cultriventris ; Microsatellite ; Fish
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 11-20
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2021-05-19
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from HardyWeinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Description: Published
    Keywords: Clupeidae ; Kilka ; Clupeonella cultriventris ; Population ; Genetic ; Microsatellite ; Fish ; DNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.139-148
    Location Call Number Limitation Availability
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