ISSN:
1432-2013
Keywords:
Key words ATP release
;
Autocrine
;
Biosensor
;
Exocytosis
;
Insulin secretion
;
P2 receptor
;
Pancreatic β cell
;
PC12 cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To examine the possibility that ATP modulates insulin secretion by an autocrine mechanism, we measured the local concentration of released ATP at the surface of a single pancreatic β cell by a new biosensor technique, using PC12 cells expressing ligand-gated cation channels, P2X2 receptors. Upon application of glucose or glibenclamide, a series of current spikes, whose amplitude equates to an ATP concentration of over 25 µM, were recorded from a PC12 cell using the whole-cell patch-clamp technique, when placed near a rat pancreatic β cell at 37°C. The current response was inhibited by cooling (below 30°C) or by applying an ATP-hydrolysing enzyme (apyrase) or a P2 receptor blocker (suramin). Thus, it is concluded that pancreatic β cells secrete ATP in response to glucose stimulation, thereby increasing the ATP concentration close to the cell surface sufficiently high enough to enhance insulin secretion from the pancreatic β cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050742
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